Bacteroides is a genus of Gram-negative, obligately anaerobic bacteria.
Bacteroides fragilis is usually a commensal organism, forming a large component of the normal human gut microbiota. However it is also an important opportunistic pathogen, with the potential to severly limit the success of gastro-intestinal surgery.
The Sanger Institute has been funded by the Wellcome Trust to sequence the genomes of two Bacteroides fragilis, strains NCTC9343 and 638R, in collaboration with Sheila Patrick (Queen's University of Belfast, UK), Garry Blakely (University of Edinburgh, UK), Val Abratt of the Department of Molecular anf Cell Biology at the University of Cape Town (South Africa), Prof. Brian Duerden (University of Wales College of Medicine, UK) and Prof. Ian Poxton (University of Edinburgh Medical School, UK).
- Single Bacterial Cell Whole Genome Amplification
- Whole genome sequencing of 1200 bacterial strains isolated from healthy faecal transplant donors and recurrent C. difficile patients
- NCTC 3000
- Global population genomics of Campylobacter fetus
- Lung Culturomics
- Human Gastrointestinal Microbiota Diversity
- Commensal bacterial genomes from health and disease
Single Bacterial Cell Whole Genome Amplification
Twenty-eight divergent polysaccharide loci specifying within- and amongst-strain capsule diversity in three strains of Bacteroides fragilis.
Microbiology (Reading, England) 2010;156;Pt 11;3255-3269
PUBMED: 20829291; PMC: 3090145; DOI: 10.1099/mic.0.042978-0
Extensive DNA inversions in the B. fragilis genome control variable gene expression.
Science (New York, N.Y.) 2005;307;5714;1463-5
PUBMED: 15746427; DOI: 10.1126/science.1107008
Data Use Statement
This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.
Please address all sequencing enquiries to: firstname.lastname@example.org