SMIS ( Single Molecular Integrative Scaffolding ): an assembly pipeline to improve scaffolds using Oxford Nanopore or PacBio long reads.
SMIS ( Single Molecular Integrative Scaffolding ): an assembly pipeline to improve genome scaffolding using Oxford Nanopore or PacBio long reads.
# Long reads are shreded into small segments (1000bp or 2000bp) to make fake mate pairs
# Genome scaffolder spinner is used for scaffolding
# Say if you have an existing assembly from Illumina reads target.fasta and ONT long reads: ontreads.fastq Usage:
./smis_pipeline -nodes 20 -score 50 -len 2000 -step 200 -contig 3000 -edge 5 <ONT_fasta/q_file> <assembly_fasta/q_file> <scaffold-output.fasta_file>
nodes – number of CPUs requested;
score – minimum smith-waterman alignment score to report a hit;
len – length of fregments of fake mate pairs;
step – jump length to cut out fregments contig – minimum contig length to be included for scaffolding;
edge – minimum number of edges to confirm a merge
======== Install: ========
tar xvf smis.tar
You need to type the full path in order to make it work.
Copyright (C) 2014 – 2015 Genome Research Ltd.
Author: Zemin Ning
SMIS is free software: you can redistribute it and/or modify it
under the terms of the GNU General Public License as published by the
Free Software Foundation, either version 3 of the License, or (at your
option) any later version.
This program is distributed in the hope that it will be useful, but
WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
General Public License for more details.
You should have received a copy of the GNU General Public License along
with this program. If not, see <http://www.gnu.org/licenses/>.
If you need help or have any queries, please contact us using the details below.
For more information, please contact: Zemin Ning ( firstname.lastname@example.org ).