A combined functional annotation score of non-synonymous coding variants

CAROL is a combined functional annotation score of non-synonymous coding variants.


A major challenge in interpreting whole-exome data is predicting which of the discovered variants are deleterious or neutral.

To address this question in silico, we have developed a score called Combined Annotation scoRing toOL (CAROL), which combines information from two bioinformatics tools: PolyPhen-2 and SIFT, in order to improve the prediction of the effect of non-synonymous coding variants. The combination of annotation tools can help improve automated prediction of whole-genome/exome non-synonymous variant functional consequences.


System requirements

The software should run on any UNIX or GNU/Linux system.

Download CAROL

After downloading the files, please read the CAROL_README.txt file to set up and run the files.

Further information

This is the licensing and citation area. Describe the terms of licence for using the software – such as creative commons.

Also give information on how you wish the software or database to be credited/cited in research papers and other literature.


If you need help or have any queries, please contact us using the details below.

If you have any problems running CAROL, then please contact either: Margarida Lopes or Eleftheria Zeggini.

It is recommended that you contact the author of CAROL, Margarida Lopes regarding bugs or problems running the script.

External Contributors

Margarida Lopes