Otter

Otter is an interactive, graphical, genome annotation tool used by the Havana group to produce high-quality gene models. (Otterlace was renamed to Otter in October 2015.)

An interactive, graphical annotation tool.

Otter enables human annotators to compare numerous sources of evidence and then to build gene objects with multiple alternative transcripts. Otter works in symbiosis with the ZMap genome browser and the SeqTools suite to provide a complete integrated annotation workbench.

The principal users of Otter are the annotators of the HAVANA group, but it is also used by annotators around the world.

Otter orchestrates, enables and validates the annotation process. Annotation is stored in 'otter' databases which use an extended EnsEMBL schema.

All of Otter's interactions with the otter databases and with external data sources are carried out over the Internet using HTTP, which allows annotators to work anywhere in the world.

Much of the annotation produced by Otter is published via the VEGA database and website.

Features

  • Browse by species, chromosome and clone.
  • Powerful search by gene, clone or evidence name or alias.
  • Flexible selection of evidence columns.
  • Transcript building by cut-and-paste of evidence.
  • Validation of splicing, translation and biotype.
  • Annotation by controlled vocabularies and by free-form comments.

Evidence sources

  • Local databases storing results of our analysis pipelines alongside the otter databases.
  • Databases maintained by the EnsEMBL team.
  • DAS sources.
  • Databases in PSL (UCSC / blat) format.
  • BAM files.

Supported platforms

Currently supported platforms are Linux and Mac OS X (Intel).

Further information

A user manual is available, written by Charlie Steward.

Otter is free software and is distributed under the terms of the Apache Version 2.0 License.

Otter should be credited to “Genome Research Ltd.”

Contact

If you need help or have any queries, please contact us using the details below.

Otter is maintained by the Annosoft team at the Sanger Institute.

If you have a bug or feature request, please raise a ticket by emailing anacode.

For any other enquires, please email the Annosoft team.


Sanger Institute Contributors

Previous contributors

Photo of Dr Adam Frankish

Dr Adam Frankish

Principal Computer Biologist

Photo of Dr Ed Griffiths

Dr Ed Griffiths

Former Senior Scientific Manager