Theileria annulata

Theileria annulata is a tickborne protozoan pathogen which is a major cause of livestock disease in sub-tropical regions with greater that 250 million cattle at risk.

Data Downloads

Both whole genome shotgun sequencing and EST sequencing of T. annulata have been done at the Sanger Institute.

Published Genome Data
Theileria annulata Whole Genome Shotgun

The Sanger Institute fully sequenced the genome of T. annulata in a collaborative project with Professor Andy Tait, Professor Brian Shiels at the University of Glasgow and Professor Declan McKeever at the Moredun Research Institute, Edinburgh. T. annulata has a 8.3 megabase genome, comprising of four haploid chromosomes. The intention was to shotgun sequence the whole genome of the Ankara clone C9. The whole genome shotgun was carried out to a 8 x coverage. Shotguns of individual chromosomes was performed to a further 2 x coverage. BAC libraries were also prepared and BAC end sequencing to 10 x coverage was carried out to further aid assembly. Information from the EST project has greatly aided gene identification in the finished sequence.

Theileria annulata EST Sequencing

Ten thousand reads from a cDNA library from Theileria annulata have being generated with the aim to identify genes involved in pathogenicity, and possible drug targets.. The project was carried out as part of a research program at the Department of Infectious Diseases and Immunology of Utrecht University and is supported by the Netherlands Foundation for the advancement of Tropical Research and the Dutch National Organization for Scientific Research.

Bibliography

  • Genome of the host-cell transforming parasite Theileria annulata compared with T. parva.

    Pain A, Renauld H, Berriman M, Murphy L, Yeats CA, Weir W, Kerhornou A, Aslett M, Bishop R, Bouchier C, Cochet M, Coulson RM, Cronin A, de Villiers EP, Fraser A, Fosker N, Gardner M, Goble A, Griffiths-Jones S, Harris DE, Katzer F, Larke N, Lord A, Maser P, McKellar S, Mooney P, Morton F, Nene V, O'Neil S, Price C, Quail MA, Rabbinowitsch E, Rawlings ND, Rutter S, Saunders D, Seeger K, Shah T, Squares R, Squares S, Tivey A, Walker AR, Woodward J, Dobbelaere DA, Langsley G, Rajandream MA, McKeever D, Shiels B, Tait A, Barrell B and Hall N

    Science (New York, N.Y.) 2005;309;5731;131-3

    PUBMED: 15994557; DOI: 10.1126/science.1110418

Related links

Data Use Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.

Sequencing enquiries

Please address all sequencing enquiries to: pathinfo@sanger.ac.uk

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