The Potato Cyst Nematodes (PCN), Globodera pallida and Globodera rostochiensis are the most economically important nematode problems of the U.K. potato industry. They occur in 65% of U.K. potato land with G. pallida present at 92% of these sites. PCN impose an annual cost in excess of £50 million on U.K. potato growers and threaten the future of the crop for many growers. Breeding for resistance since 1950s has produced a few commercially acceptable varieties with resistance to G. pallida. Effective control of G. pallida is an essential requirement to maintain the competitiveness of U.K. potato production. The aim of this project is to obtain the full genome sequence of G. pallida to aide in defining new control measures and pest management strategies.
This is a BBSRC funded project to sequence and and analyse the nuclear and mitochondrial genomes of G. pallida with the goal of producing a reference quality genome sequence. The sequencing is being done using a combination of capillary sequencing, Roche (454) and Illumina (Solexa) sequencing technology platforms. As part of the project we are profiling the transcriptome of the parasite across its life cycle using RNA-seq.
We are collaborating with John Jones and Vivian Blok and Peter Cock (The James Hutton Institute), Brian Kerry (Rothamsted Research), Catherine Lilley and Peter Urwin (University of Leeds).
Data Use Statement
This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.
Please address all sequencing enquiries to: firstname.lastname@example.org