Haemophilus

Haemophilus is a genus of Gram-negative, pleomorphic, coccobacilli bacteria belonging to the Pasteurellaceae family, including common commensals of the human upper respiratory tract, some of which are associated with human disease. H. influenzae, and rarely H. parainfluenzae, cause numerous mild and serious infections, including bacteremia, meningitis, pneumonia and otitis media.

Data Downloads

• FTP site

• BLAST server

The Sanger Institute has been funded by the Wellcome Trust to sequence the genomes of

Haemophilus influenzae serotype B strain 10810

Haemophilus influenzae biogroup Aegypticus BPF strain F3031

Haemophilus influenzae biogroup Aegypticus conjunctivitis strain F3047

Haemophilus parainfluenzae strain T3T1

Collaborators

Dr. Derrick Crook of the University of Oxford Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford

Dr. Derek Hood and Prof. Richard Moxon of the University of Oxford Department of Paediatrics, John Radcliffe Hospital, Oxford

Professor Simon Kroll of the Department of Paediatric Infectious Diseases at Imperial College, London

Also in collaboration with Dr. Derek Hood and Dr. Peter Power of the University of Oxford Department of Paediatrics, John Radcliffe Hospital, Oxford we have initiated a Wellcome Trust funded project to study population structure in the genus Haemophilus and mechanisms of recombination in Haemophilus influenzae and Neisseria meningitidis.

Published Genome Data

We have completed the details of several strains of Haemophilus, the details of which are given below.

Finished Sequence

Haemophilus influenzae strain 10810

The genome of Haemophilus influenzae 10810is 1,981,535 and has a G+C content of 38.14%. The data is available for searching on our BLAST server or for download from our FTP site and from EMBL/GenBank with accession number FQ312006.

Haemophilus influenzae strain F3031

We have completed the genome of Haemophilus influenzae biogroup Aegypticus BPF strain F3031. Biogroup Aegypticus is normally associated with purulent conjuctivitis. Strain F3031 is a representative of a clone associated with the Brazilian purpuric fever outbreak in the early 1980s. The genome consists of a single chromosome of 1,985,824 bp with a G+C content of 38.22% and a plasmid of 32,433 bp.

The genome is available from EMBL/GenBank with accession number FQ670178.

Haemophilus parainfluenzae strain T3T1

Haemophilus parainfluenzae is a Gram negative commensal of the human respiratory tract. Strain T3T1 was obtained from the throat of a healthy child, and has been confirmed by 16S rRNA sequence. The genome is now finished and is 2,086,875 bp in size with a G+C content of 39.57%.

The genome is available from EMBL/GenBank with accession number FQ312002.

Shotgun and assembly data from these projects are available from our FTP site.

Ongoing Projects

Haemophilus influenzae strain F3047

Haemophilus influenzae is a Gram negative commensal of the human respiratory tract that can cause meningitis and other diseases. Strain F3047 is a representative of biogroup Aegypticus which is normally associated with purulent conjuctivitis.

At present there are 31,070 reads totalling 16.797 Mb and giving a theoretical coverage of 99.95% of the genome. There are 7 contigs > 1kb (3 contigs >2kb) with a total size of 2.207 Mb. Finishing of this genome is in progress.

Related links

• Artemis
• Bacteria
• University Department of Paediatrics, Oxford
• Nuffield Department of Clinical Laboratory Sciences
• WTSI Sequencing

Data Use Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.

Sequencing enquiries

Please address all sequencing enquiries to: pathinfo@sanger.ac.uk