Clostridium

Bacteria of the genus Clostridium are Gram positive, obligate anaerobes capable of producing endospores. Species include:

  • Clostridium botulinum which produces botulinum neurotoxin, which is the cause of foodborne botulism.
  • Clostridium sordellii, a gram-positive, spore forming anaerobic bacterium associated with rare but severe infections of both humans and animals.

Please Note: Due to recent changes in phylogeny and taxonomy, data from Clostridium difficile formerly found on this page can now be found on the Peptoclostridium difficile page.

Data Downloads

Clostridium botulinum

The Sanger Institute has completed the sequence of the genome of Clostridium botulinum, in collaboration with Dr. Roger Huston of the Food Microbial Sciences Unit, University of Reading Department of Food Science, Dr. Nigel Minton of the Institute of Infection, Immunity and Inflammation, University of Nottingham, and Dr. M. Peck of the Food Saftey Microbiology Section, Institute of Food Research.

We have sequenced Hall strain A (ATCC 3502) and Eklund 17B. The genome of Hall strain A (ATCC 3502) is 3,886,916 bp in size, with a G+C content of approximately 28.2%. There is also a plasmid of 16,344 bp. The completed sequence for both strains is available for searching on our Blast server, or for download from our FTP site. This project is funded by Beowulf Genomics.

The fully annotated genome and plasmid are available of Hall strain A (ATCC 3502) are available from the EMBL/GenBank databases with accession numbers AM412317 and AM412318 respectively.

Shotgun and assembly data from this project are available from our FTP site.

Clostridium sordellii

We have completed the sequencing, assembly and annotation of 44 diverse strains of Clostridium sordellii. All are isolated from incidences of clinical or veterinary disease and are from diverse geographical sources- see table below. This work was carried out in collaboration with Professor Neil Fairweather of Imperial College London. An Optical Map was generated for strain ATCC9714 here at the WTSI to improve and validate the initial genome assembly in addition to isolating plasmid sequence.

For full details of all sequenced strains and links to the annotated assemblies please see the C. sordellii page.

Studies

MetaHIT Genome Improvement
SampleStrainRun Accession
C_saccharolyticum_like_K10K10ERR037811
250510-53UnknownERR043353
SampleStrainRun Accession
2305_30230511_30ERR056965
1805pm_95180511pm_95ERR056982
16JANC8180511pm_93ERR152469
2709am_56230511_30ERR152454
2709am_1230511_25ERR152453
Unable to read file: /resources/downloads/bacteria/inc/clostridium2047.inc
Unable to read file: /resources/downloads/bacteria/inc/clostridium2789.inc
NCTC 3000
SampleStrainRun Accession
3012STDY6324663NCTC8678ERR1377187
3012STDY6324665NCTC8797ERR1377188
3012STDY6324667NCTC13170ERR1377189
3012STDY6345025NCTC8503ERR1407347
3012STDY6383822NCTC503ERR1456744
3012STDY6383823NCTC2544_1ERR1456745
3012STDY6387765NCTC8799ERR1466824
3012STDY6430049NCTC10612_1ERR1588634
3012STDY6439499NCTC3182ERR1599940
3012STDY6439518NCTC8238ERR1656456
3012STDY6447001NCTC8246ERR1656458
3012STDY6447002NCTC2837ERR1656459
3012STDY6447003NCTC8081ERR1656460
3012STDY6447004NCTC8247ERR1674568
3012STDY6451290NCTC8239ERR1681948
3012STDY6451291NCTC9851ERR1681949
3012STDY6451292NCTC10239ERR1681950
3012STDY6451293NCTC10240ERR1681951
3012STDY6496191NCTC11861ERR1787518
3012STDY6496194NCTC11811ERR1787522
3012STDY6542262NCTC8679ERR1787549
3012STDY6542263NCTC10578ERR1787550
3012STDY6542264NCTC10613ERR1787552
3012STDY6542265NCTC10614ERR1800584
3012STDY6551389NCTC8359ERR1805687
3012STDY6562792NCTC11832ERR1820603
3012STDY6481860NCTC507ERR1802399
3012STDY6481866NCTC11035ERR1800575
3012STDY6590992NCTC8449ERR1893260
3012STDY6590993NCTC8798ERR1883086
3012STDY6590995NCTC10719ERR1892097
3012STDY6590996NCTC10720ERR1892098
3012STDY6593597NCTC8380ERR1892121
3012STDY6593598NCTC541ERR1892122
3012STDY6593603NCTC13020ERR1892108
3012STDY6593608NCTC11210ERR1901077
3012STDY6606161NCTC279ERR1911789
3012STDY6606162NCTC13233ERR1911790
3012STDY6617316UnknownERR2532409
3012STDY6617317UnknownERR1940990
3012STDY6617320UnknownERR1948259
3012STDY6617321UnknownERR1948260
3012STDY6617334UnknownERR1954484
3012STDY6622196NCTC13356ERR2532413
3012STDY6622197NCTC10986_1ERR1954500
3012STDY6632826NCTC276ERR2125613
3012STDY6632827NCTC275ERR2125614
3012STDY6632830NCTC506_1ERR2125617
3012STDY6632834NCTC1341_1ERR2125618
3012STDY6632836NCTC539ERR2125619
3012STDY6632837NCTC534ERR2125620
3012STDY6632838NCTC2909ERR2125621
3012STDY6649824NCTC8237ERR2125640
3012STDY6649825NCTC543_1ERR2125641
3012STDY6667051NCTC9836ERR2125690
3012STDY6667054NCTC540_1ERR2125692
3012STDY6674172NCTC4964ERR2125710
3012STDY6680706NCTC13029ERR2125732
3012STDY6733928NCTC13037ERR2125766
3012STDY6733932NCTC10913ERR2125774
3012STDY6733942NCTC13028ERR2125788
3012STDY6719852NCTC11224ERR2225526
3012STDY7078513NCTC13108ERR2246680
3012STDY7122774NCTC13501ERR2532468
3012STDY7103850NCTC500ERR2432896
3012STDY7103851NCTC4718ERR2432897
Unable to read file: /resources/downloads/bacteria/inc/clostridium3336.inc
Unable to read file: /resources/downloads/bacteria/inc/clostridium3542.inc
Lung Culturomics
SampleStrainRun Accession
9_Run_RUnknownERR968252
Unable to read file: /resources/downloads/bacteria/inc/clostridium4186.inc
Unable to read file: /resources/downloads/bacteria/inc/clostridium4237.inc
Unable to read file: /resources/downloads/bacteria/inc/clostridium4530.inc

Bibliography

  • Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes.

    Sebaihia M, Peck MW, Minton NP, Thomson NR, Holden MT, Mitchell WJ, Carter AT, Bentley SD, Mason DR, Crossman L, Paul CJ, Ivens A, Wells-Bennik MH, Davis IJ, Cerdeño-Tárraga AM, Churcher C, Quail MA, Chillingworth T, Feltwell T, Fraser A, Goodhead I, Hance Z, Jagels K, Larke N, Maddison M, Moule S, Mungall K, Norbertczak H, Rabbinowitsch E, Sanders M, Simmonds M, White B, Whithead S and Parkhill J

    Genome research 2007;17;7;1082-92

Data Use Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.

Sequencing enquiries

Please address all sequencing enquiries to: pathinfo@sanger.ac.uk

Short-link