Bacteria of the genus Clostridium are Gram positive, obligate anaerobes capable of producing endospores. Species include:
- Clostridium botulinum which produces botulinum neurotoxin, which is the cause of foodborne botulism.
- Clostridium sordellii, a gram-positive, spore forming anaerobic bacterium associated with rare but severe infections of both humans and animals.
The Sanger Institute has completed the sequence of the genome of Clostridium botulinum, in collaboration with Dr. Roger Huston of the Food Microbial Sciences Unit, University of Reading Department of Food Science, Dr. Nigel Minton of the Institute of Infection, Immunity and Inflammation, University of Nottingham, and Dr. M. Peck of the Food Saftey Microbiology Section, Institute of Food Research.
We have sequenced Hall strain A (ATCC 3502) and Eklund 17B. The genome of Hall strain A (ATCC 3502) is 3,886,916 bp in size, with a G+C content of approximately 28.2%. There is also a plasmid of 16,344 bp. The completed sequence for both strains is available for searching on our Blast server, or for download from our FTP site. This project is funded by Beowulf Genomics.
Shotgun and assembly data from this project are available from our FTP site.
We have completed the sequencing, assembly and annotation of 44 diverse strains of Clostridium sordellii. All are isolated from incidences of clinical or veterinary disease and are from diverse geographical sources- see table below. This work was carried out in collaboration with Professor Neil Fairweather of Imperial College London. An Optical Map was generated for strain ATCC9714 here at the WTSI to improve and validate the initial genome assembly in addition to isolating plasmid sequence.
For full details of all sequenced strains and links to the annotated assemblies please see the C. sordellii page.
- MetaHIT Genome Improvement
- Whole genome sequencing of pathogenic strains of Mycobacterium abscessus
- Generation of draft genomes for supershedder and bacteriotherapy bacteria
- Clostridium difficile 078 diversity
- Neglected Clostridial pathogens
- Whole genome sequencing of 1200 bacterial strains isolated from healthy faecal transplant donors and recurrent C. difficile patients
- NCTC 3000
- Early life microbiota colonisation
- Global population genomics of Campylobacter fetus
- Lung Culturomics
- Human Gastrointestinal Microbiota Diversity
- Clostridium perfringens- Phylogenetic and virulence analysis of Clostridium species primarily from pre-term infantsI
- Commensal bacterial genomes from health and disease