Campylobacter jejuni is the leading cause of food poisoning, being three times more common than Salmonella.
The genome sequence of C. jejuni was produced in collaboration with Brendan Wren of the London School of Hygiene and Tropical Medicine and Dr. Julian Ketley of the Department of Genetics, University of Leicester. It was sequenced using a whole genome shotgun approach using several 1.4-2.2 kb pUC18 libraries, both generated in-house, and supplied by Andrey V. Karlyshev from Dr. Wren's laboratory.
Reannotation of the Campylobacter jejuni NCTC11168
In August 2006, Ozan Gundogdu from The London School of Hygiene & Tropical Medicine in collaboration with the Sanger Institute, carried out a complete re-annotation of the NCTC11168 genome. The re-annotation has added new information to over 1450 of the original 1654 coding sequences with over 300 product functions being updated. Additional Campylobacter work carried out in Brendan Wren's group can be accessed from the Campylobacter Resource Facility homepage.
Published Genome Data
The complete sequence is 1,641,481 bp in length (with 25 polymorphic regions) and was generated from 33,824 sequencing reads. It starts at the first base in the dnaA gene, close to the origin of replication. Both the sequence and annotation have been deposited in the public databases with the ID CJ11168 (accession number AL111168). Shotgun and assembly data from this project are available from our ftp://ftp.sanger.ac.uk/pub/pathogens/cj/[ FTP site].
We have also generated sequence data from C. jejuni M1 using the 454/Roche GS20 system. The sequence is in 624 contigs with a total size of 1.619 Mb and may be downloaded from our ftp://ftp.sanger.ac.uk/pub/pathogens/cj/[ FTP site]. Please note that this sequence is unchecked and unedited, and will contain errors. The GS20 is known to have problems with homopolymeric tracts, and these are therefore likely to contain significant numbers of errors.
The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences.
Data Use Statement
This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.
Please address all sequencing enquiries to: firstname.lastname@example.org