Burkholderia
Burkholderia is a genus of Proteobacteria. It's pathogenic members include:
- Burkholderia cenocepacia (formerly Burkholderia cepacia genomovar III, and before that Pseudomonas cepacia), a Gram negative bacterium which is ubiquitous in the environment and may cause a number of diseases in plants. Human infection can be caused by B. cepacia, especially in patients with cystic fibrosis and chronic granulomatous disease, and is often fatal.
- Burkholderia pseudomallei (previously called Pseudomonas pseudomallei), the causative agent of melioidosis, a serious disease of man and animals that occurs primarily in S.E. Asia, N. Australia and other tropical areas. B. pseudomallei is an environmental Gram-negative saprophyte present in wet soil and rice paddies in endemic areas. The highest documented infection rate is in northeastern Thailand, where melioidosis accounts for 20% of all community-acquired septicaemias. Disease occurs after bacterial contamination of breaks in the skin or by inhalation after contact with water or soil. There is no licensed vaccine against meliodosis, and the bacterium is resistant to many antibiotics. B. pseudomallei strain K96243 is a clinical isolate from Thailand, supplied by Dr. S. Songsivilai of Mahidol University.
Data Downloads
Burkholderia cenocepacia
The Sanger Institute has completed the sequencing of and published the 8.06 Mb genome of Burkholderia cenocepacia strain J2315, in collaboration with Dr. Eshwar Mahenthiralingham of the School of Biosciences, Cardiff University, Prof. John Govan of the Department of Medical Microbiology, University of Edinburgh, Prof. C.A. Hart of the Department of Medical Microbiology, University of Liverpool, and Prof. Peter Vandamme of the Laboratorium voor Microbiologie, Universiteit Gent, Belgium.
Note that B. cenocepacia J2315 has recently been renamed from B. cepacia J2315.
The sequenced strain, J2315, is a genomovar III strain of the ET12 lineage, and is the index strain for transmission of this strain among CF patients in the UK. Strain J2315 has been deposited as LMG 16656 in the Belgium Coordinated Collection of Microorganisms.
The sequence and annotation of the Burkholderia cenocepacia strain J2315 genome have been deposited in the EMBL database under accession numbers AM747720, AM747721, AM747722 and AM747723
Shotgun and assembly data from this project are available from our FTP site.
Burkholderia pseudomallei
The Sanger Institute has published the 7.2 Mb genome of Burkholderia pseudomallei in collaboration with Dr. Rick Titball of the Defence Science and Technology Laboratory, Porton Down, and Dr. Ty Pitt of the Heath Protection Agency. We have sequenced strain K96243, a clinical isolate from Thailand, supplied by Dr. S. Songsivilai of Mahidol University. This project is funded by Beowulf Genomics.
The reference genome is 7,247,547 bp in size with a G+C content of 68.06% consisting of two chromosomes of 4,074,542 bp and 3,173,005 bp. The sequence is available for download by FTP and can be searched using our BLAST server.
The two fully annotated chromosomal sequences are deposited in the EMBL/GenBank databases with accession numbers BX571965 and BX571966.
Shotgun and assembly data from this project are available from our FTP site.
Studies
- Discovery of sequence diversity in Burkholderia pseudomallei infection
- Burkholderia pseudomallei diversity
- Burkholderia pseudomallei - evolutionary origin and spread
- HPACC Murray project
- Burkholderia pseudomallei MLST determination
- Burkholderia pseudomallei - case-control study
- Sequencing Burkholderia gladioli BCC0238
- NCTC 3000
- Hospital acquired Klebsiella pneumoniae and the role of colonization and transmission (Australia)
- Bloodstream surveillence across Africas
- Lung Culturomics
- PacBio sequencing of Burkholderia ambifaria
- Exploitation of Burkholderia bacteria as novel antibiotic producers using a genome mining approach
- Burkholderia stabilis- understanding the basis of Burkholderia pathogenicty using a mutable isolate
Discovery of sequence diversity in Burkholderia pseudomallei infection
Burkholderia pseudomallei diversity
Burkholderia pseudomallei - evolutionary origin and spread
HPACC Murray project
Burkholderia pseudomallei MLST determination
Sample | Strain | Run Accession |
---|---|---|
BP2_ab | Unknown | ERR230473 |
BP1_bc | Unknown | ERR230472 |
Burkholderia pseudomallei - case-control study
Sequencing Burkholderia gladioli BCC0238
Sample | Strain | Run Accession |
---|---|---|
BCC0238_A | Unknown | ERR353354 |
Hospital acquired Klebsiella pneumoniae and the role of colonization and transmission (Australia)
Sample | Strain | Run Accession |
---|---|---|
3248STDY6094065 | Unknown | ERR1015396 |
Lung Culturomics
Sample | Strain | Run Accession |
---|---|---|
4_Run_R | Unknown | ERR968248 |
PacBio sequencing of Burkholderia ambifaria
Sample | Strain | Run Accession |
---|---|---|
3723STDY6145927 | BCC0203 | ERR1036250 |
3723STDY6145927 | BCC0203 | ERR1036251 |
3723STDY6145927 | BCC0203 | ERR1046620 |
Bibliography
-
The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients.
Journal of bacteriology 2008;191;1;261-77
PUBMED: 18931103; PMC: 2612433; DOI: 10.1128/JB.01230-08
-
Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei.
Proceedings of the National Academy of Sciences of the United States of America 2004;101;39;14240-5
PUBMED: 15377794; PMC: 521101; DOI: 10.1073/pnas.0403302101
-
Burkholderia cenocepacia sp. nov.--a new twist to an old story.
Research in microbiology 2003;154;2;91-6
PUBMED: 12648723; DOI: 10.1016/S0923-2508(03)00026-3
Related links
Data Use Statement
This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.
Sequencing enquiries
Please address all sequencing enquiries to: pathinfo@sanger.ac.uk