Burkholderia pseudomallei (previously called Pseudomonas pseudomallei) is the causative agent of melioidosis, a serious disease of man and animals that occurs primarily in S.E. Asia, N. Australia and other tropical areas. B. pseudomallei is an environmental Gram-negative saprophyte present in wet soil and rice paddies in endemic areas. The highest documented infection rate is in northeastern Thailand, where melioidosis accounts for 20% of all community-acquired septicaemias. Disease occurs after bacterial contamination of breaks in the skin or by inhalation after contact with water or soil. There is no licensed vaccine against meliodosis, and the bacterium is resistant to many antibiotics. B. pseudomallei strain K96243 is a clinical isolate from Thailand, supplied by Dr. S. Songsivilai of Mahidol University.
The Sanger Institute has published the 7.2 Mb genome of Burkholderia pseudomallei in collaboration with Dr. Rick Titball of the Defence Science and Technology Laboratory, Porton Down, and Dr. Ty Pitt of the Heath Protection Agency. We have sequenced strain K96243, a clinical isolate from Thailand, supplied by Dr. S. Songsivilai of Mahidol University. This project is funded by Beowulf Genomics.
Published Genome Data
The reference genome is 7,247,547 bp in size with a G+C content of 68.06% consisting of two chromosomes of 4,074,542 bp and 3,173,005 bp. The sequence is available for download by FTP and can be searched using our BLAST server.
The two fully annotated chromosomal sequences are deposited in the EMBL/GenBank databases with accession numbers BX571965 and BX571966.
Shotgun and assembly data from this project are available from the FTP link sbove.
Discovery of sequence diversity in Burkholderia pseudomallei infection
Burkholderia pseudomallei diversity
Burkholderia pseudomallei - evolutionary origin and spread
HPACC Murray project
Burkholderia pseudomallei MLST determination
Burkholderia pseudomallei - case-control study
Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei.
Proceedings of the National Academy of Sciences of the United States of America 2004;101;39;14240-5
PUBMED: 15377794; PMC: 521101; DOI: 10.1073/pnas.0403302101
Data Use Statement
This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.
Please address all sequencing enquiries to: firstname.lastname@example.org