An obligate anaerobe, Bacteroides fragilis is usually a commensal organism, forming a large component of the normal human gut microbiota. However it is also an important opportunistic pathogen, with the potential to severly limit the success of gastro-intestinal surgery.
The Sanger Institute has been funded by the Wellcome Trust to sequence the genomes of two Bacteroides fragilis, strains NCTC9343 and 638R, in collaboration with Sheila Patrick (Queen's University of Belfast, UK), Garry Blakely (University of Edinburgh, UK), Val Abratt of the Department of Molecular anf Cell Biology at the University of Cape Town (South Africa), Prof. Brian Duerden (University of Wales College of Medicine, UK) and Prof. Ian Poxton (University of Edinburgh Medical School, UK).
Published Genome Data
We have sequenced, annotated and analysed the complete genome of the type strain NCTC9343 (ATCC 25285). The genome is 5,205,140 bp long, with a G+C content of 43.19%. The data are available for download by FTP and can be searched using our BLAST server. The annotated data are also available from EMBL/GenBank with accession number CR626927.
We have also sequenced, annotated and analysed the complete genome of strain 638R. The data from strain 638R are available for download by FTP and can be searched using our BLAST server. The sequence is 5,373,121 bp with a G+C content of 43.42%. The shotgun data are still available: There are 81,661 reads in the database, totalling 46.520 Mb, and giving a theoretical coverage of 99.99%. The annotated data are also available from EMBL/GenBank with accession number FQ312004.
Twenty-eight divergent polysaccharide loci specifying within- and amongst-strain capsule diversity in three strains of Bacteroides fragilis.
Microbiology (Reading, England) 2010;156;Pt 11;3255-3269
PUBMED: 20829291; PMC: 3090145; DOI: 10.1099/mic.0.042978-0
Extensive DNA inversions in the B. fragilis genome control variable gene expression.
Science (New York, N.Y.) 2005;307;5714;1463-5
PUBMED: 15746427; DOI: 10.1126/science.1107008
Data Use Statement
This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.
Please address all sequencing enquiries to: firstname.lastname@example.org