Wellcome Sanger Institute

Cellular and Gene Editing Research

Cellular Operations

We develop novel genome editing techniques, cellular differentiation and cellular phenotyping systems, especially with respect to high-throughput investigation of gene and non-coding regulatory element function. 

  • Developing induced pluripotent stem cell models of genetic disease
  • Genetic screening techniques using pooled and arrayed CRISPR/Cas9
  • Differentiation protocols of pluripotent stem cells to brain and blood-derived lineages
  • Application of screening to complex phenotypic readouts such as single cell transcriptomics or spatial genomics 

We develop novel cutting-edge CRISPR screening techniques such as saturation mutagenesis, base editing, dual guide libraries and CRISPRa/i and couple these to complex phenotypic readouts such as single cell transcriptomics, spatial genomics or phenotypic assays of cellular function. We also work with and develop human cellular systems and differentiation protocols of iPSCs in both 2D and 3D to better model human disease states in vitro. The systems developed will be applied to probe the genetic basis of cellular function in health and disease and understand the underlying cell biology.

Core team

Photo of Dr Erica Bello

Dr Erica Bello

Postdoctoral Fellow

Photo of Mrs Michaela Bruntraeger

Mrs Michaela Bruntraeger

Senior Research Assistant

Photo of Thomas Burgold

Thomas Burgold

Staff Scientist

Photo of Dr Yixi Chen

Dr Yixi Chen

Postdoctoral Fellow

Photo of Dr Sarah Cooper

Dr Sarah Cooper

Senior Staff Scientist

Photo of Dr Caia Dominicus

Dr Caia Dominicus

Postdoctoral Fellow

Photo of Dr Daniel Gitterman

Dr Daniel Gitterman

Staff Scientist

Photo of Dr Valentina Migliori

Dr Valentina Migliori

Staff Scientist

Photo of Dr Diego A. Peretti

Dr Diego A. Peretti

Postdoctoral Fellow

Photo of Dr Sam Washer

Dr Sam Washer

Postdoctoral Fellow

Photo of Dr Qianxin Wu

Dr Qianxin Wu

Staff Scientist

Previous team members

Photo of Dr Meg Byrne, EngD

Dr Meg Byrne, EngD

Senior Research Assistant

Photo of Jeremy Schwartzentruber

Jeremy Schwartzentruber

Former PhD Student at the Sanger Institute

Partners

We work with the following groups

External

OpenTargets

Open Targets is an innovative, large-scale, multi-year, public-private partnership that uses human genetics and genomics data for systematic drug target identification and prioritisation.

 

Publications

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