Lawniczak Group

Evolutionary genetics

Our team works across three main Themes of scientific research--in each Theme, we strive to have positive impacts in global health. 

Through our work on Mosquito Vector Population Genomics and on the Malaria Cell Atlas, we aim to improve malaria control while also enhancing our understanding of evolution. Similarly, our work on Insect Biodiversity will build a foundational resource for large scale DNA-based biomonitoring while also revealing new evolutionary dynamics. 

We use a variety of genomic tools from short and long read whole genome sequencing to single cell RNAseq to DNA barcoding. Our three research Themes are described in more detail below.

Theme: Mosquito Vector Population Genomics

Species diversity in Anopheles: We are focused on a deeper understanding of diversity in malaria mosquito vectors — in particular, across Africa. We have built the simple amplicon panel ANOSPP that can be used on any Anopheline to diagnose species, identify geographic origin, and determine if the mosquito is positive for Plasmodium parasites and if so, which species — we are always seeking new partners for this project, please check out our project page.

Anopheles funestus population genomics: We lead the Anopheles funestus population genomics project to evaluate diversity and selection in this major malaria vector. This is in collaboration with many scientists across Africa. We are using whole genome sequence data generated from hundreds of specimens collected across Africa to explore population structure, understand selection, and improve the chances for gene drive success in the face of the vast amount of genetic diversity that segregates within African Anopheles species.

Project Neandersquito: To understand how populations have changed over time, we are generating whole genome sequence data from pinned museum specimens of mosquitoes collected over the past century. We do this in a non-destructive way (otherwise curators would never let us touch their collections!).

High quality reference genomes: A good reference genome unlocks huge potential towards the study of an organism, and technology now makes this possible using single small insects. With support from the Bill and Melinda Gates Foundation, we have generated high quality reference genomes for over ten understudied Anopheles vector species around the world using long-read and long-range sequencing technologies. This project remains open to produce more reference genomes for the community — please get in touch if your Anopheles species needs a high quality reference genome.

Theme: Transmission biology of Plasmodium falciparum

Malaria Cell Atlas: We have pioneered the application of single cell transcriptional approaches to investigate individual Plasmodium parasites. We have created the Malaria Cell Atlas, which is freely available and interactive and displays how any gene is expressed at any point across the entire life cycle of parasites for multiple Plasmodium species. We use the nucleotide variants from the transcriptional data to assign a genetic identity to each cell, and thereby we are deconvolving natural mixed infections for the first time. We are now working in collaboration with scientists in Mali to incorporate wild parasites from P. falciparumP. malariae, and P. ovale into our atlas.

Transmission Dynamics:  We are using single cell RNAseq to to study the behavior of P. falciparum parasites from natural infections. How do malaria parasites behave inside of the human host to enhance their probability of transmission? In particular, using single cell approaches, we are interrogating the relationship of the asexual to sexual parasites in a given infection (are all asexual parasites contributing to the transmission pool?), and studying whether parasites mate non-randomly inside the mosquito. This project is funded by the Medical Research Council.

All areas of our research on mosquitoes and parasites are focused on a deep evolutionary understanding of these interesting yet deadly organisms, and ultimately our research and the data we generate will help to implement new methods of control, including gene drive and transmission blocking strategies that aim to stop malaria.

 

Theme: Biodiversity Genomics

DToL: We are heavily involved in the Darwin Tree of Life Project, which is generating reference genomes for all described British eukaryotic species.

BIOSCAN for Flying Insects: The BIOSCAN project is using simple DNA barcoding to study insect species diversity and species interactions across the UK for 1 million insects over five years. Through our partners, we have 100 sites across the UK using Malaise traps on a monthly basis to passively collect insects so we can study how species diversity and species interactions change through space and time.

Hoverfly population genomics: Insects collected as part of BIOSCAN are an amazing resource to further study population genomics. We are exploring population structure and genome structure of hoverflies that exhibit different migratory behavior using linked and long reads. Over time, we will extend these approaches to other hyperdiverse Diptera families collected through the BIOSCAN initiative.

Core team

Photo of Marilou Boddé

Marilou Boddé

Postdoctoral Fellow

Photo of Dr Sunil Kumar Dogga

Dr Sunil Kumar Dogga

Postdoctoral Fellow

Photo of Amjad Khalaf

Amjad Khalaf

PhD Student

Photo of Dr Petra Korlevic

Dr Petra Korlevic

Staff Scientist

Photo of Dr Alex Makunin

Dr Alex Makunin

Computational Staff Scientist

Photo of Dr Joachim Nwezeobi

Dr Joachim Nwezeobi

Postdoctoral Fellow

Photo of Dr Lyndall Pereira da Conceicoa

Dr Lyndall Pereira da Conceicoa

Samples and Partner Relationship Manager

Photo of Dr Jemma Salmon

Dr Jemma Salmon

BIOSCAN Samples and Partner Relationship Lead

Photo of Edel Sheerin

Edel Sheerin

Technical Specialist 

Photo of Dr Priyanka Surana

Dr Priyanka Surana

Senior Bioinformatician

Photo of Fiona Teltscher

Fiona Teltscher

Advanced Research Assistant

Photo of Dr Katie Woodcock

Dr Katie Woodcock

Postdoctoral Fellow

Photo of Charlotte Wright

Charlotte Wright

PhD Student

Previous team members

Photo of Dr Physilia Chua

Dr Physilia Chua

Postdoctoral Fellow

Photo of Juliana Cudini

Juliana Cudini

PhD Student

Photo of Mr Cameron Robert  Ferguson

Mr Cameron Robert Ferguson

Research Assistant

Photo of Haynes Heaton

Haynes Heaton

PhD Student

Photo of Dr Virginia Muriel Howick

Dr Virginia Muriel Howick

Postdoctoral Fellow

Photo of Dr Harriet Johnson

Dr Harriet Johnson

Advanced Research Assistant

Photo of Dr Krzysztof Kozak

Dr Krzysztof Kozak

Postdoctoral Fellow

Photo of Miss Katie Love

Miss Katie Love

Advanced Research Assistant

Photo of Charles Mulamba

Charles Mulamba

Staff Scientist

Photo of Dr Silvain Pinaud

Dr Silvain Pinaud

Postdoctoral Fellow

Photo of Dr Arthur M Talman

Dr Arthur M Talman

Postdoctoral Fellow

Partners

We are funded by the MRC,the Bill and Melinda Gates Foundation, and Wellcome.

 

Publications

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