PlasmoGEM | Parasites and Microbes

PlasmoGEM | Parasites and Microbes

PlasmoGEM

Our Research and Approach

Malaria is a mosquito transmitted disease that still poses a major public health problem to a large portion of the world, with 216 million cases and nearly 0.5 million deaths in 2016. Malaria is caused by Plasmodium parasites that are transmitted to people through the bites of infected female Anopheles mosquitoes. Plasmodium are single cell eukaryotes with complex life cycles, and are only very distantly related to model eukaryotes such as yeast or nematode worms. As a result, almost two decades since the completion of the first Plasmodium reference genome, nearly 50% of the 5,000+ genes in the genome lack an annotated function.

PlasmoGEM (the Plasmodium Genetic Modification Project) is an interdisciplinary group at the Wellcome Sanger Institute focussed on changing the scale of malaria experimental genetics.

PlasmoGEM develops new genetic tools and makes them freely available to the malaria research community. It also uses those tools to carry out large-scale genetic screens, such as a recent screen that quantified blood-stage growth rates for knockouts of over half the Plasmodium berghei genome (Bushell et al., Cell 2017).

PlasmoGEM in P.berghei
Contribution of PlasmoGEM to the number of P. berghei mutants catalogued in the Rodent Malaria Genetic Modification Database (https://www.pberghei.eu/index.php)

PlasmoGEM is a collaborative project shared across the Rayner, Lee and Billker groups.

People

Boroviak, Katharina
Dr Katharina Boroviak
Group Leader

My interest and expertise lies in genome engineering and my research seeks to improve efficiency and accuracy of genome engineering tools, such as CRISPR/Cas9.

Rayner, Julian C

picture-jr9.jpg
Dr Julian C Rayner
Senior Group Leader and Director of Wellcome Genome Campus Connecting Science
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Alumni

Key Projects, Collaborations, Tools & Data

For detailed information on the P.berghei tools and phenotypic data plase go to https://plasmogem.sanger.ac.uk/

Research Programmes and Faciltites

Partners and Funders

Internal Partners

Publications

  • Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes.

    Bushell E, Gomes AR, Sanderson T, Anar B, Girling G et al.

    Cell 2017;170;2;260-272.e8

  • A genome-scale vector resource enables high-throughput reverse genetic screening in a malaria parasite.

    Gomes AR, Bushell E, Schwach F, Girling G, Anar B et al.

    Cell host & microbe 2015;17;3;404-413

  • PlasmoGEM, a database supporting a community resource for large-scale experimental genetics in malaria parasites.

    Schwach F, Bushell E, Gomes AR, Anar B, Girling G et al.

    Nucleic acids research 2015;43;Database issue;D1176-82

  • A scalable pipeline for highly effective genetic modification of a malaria parasite.

    Pfander C, Anar B, Schwach F, Otto TD, Brochet M et al.

    Nature methods 2011;8;12;1078-82