My interest and expertise lies in genome engineering and my research seeks to improve efficiency and accuracy of genome engineering tools, such as CRISPR/Cas9.

After my PhD in the laboratory of Dr. Henry Roehl at the University of Sheffield working on bone development in zebrafish, I took up a position as the Facility Manager of the Advanced Vector Design and BAC Recombineering Facility at the Wellcome Trust – Medical Research Council Stem Cell Institute (WT-MRC-SCI) in Cambridge. There we utilised BAC recombineering, Transcription activator-like effector nucleases (TALENs) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) to generate custom made targeting constructs for different faculty projects. During my time there I developed a keen interest in genome engineering.

In 2013 I joined the laboratory of Prof. Allan Bradley to work on CRISPR/Cas9 mediated genome engineering in mouse zygotes. I optimized the method in order to generate mouse models in the most efficient and effective manner. I also worked on determining the limitations of the CRISPR/Cas9 system and established protocols to generate models carrying indels (small insertions or deletions), point mutations (SNPs), genomic rearrangements (deletions, inversions, duplications) or Knock-Ins.

In 2016 I started managing part of the Bespoke Mouse Genome Engineering Team. The aim of this team is to generate mutant mouse lines for the research community. The team uses CRISPR/Cas9 in mouse zygotes to generate critical exon deletions and introduce point mutations (SNPs). CRISPR/Cas9 is also used in mouse embryonic stem cells (mESC) to generate more complex alleles such as knock-in of larger constructs (LacZ, GFP, COIN modules).

I took up the position of team leader of PlasmoGEM in 2018. I am hoping to put my expertise in genome engineering to good use in generating freely available tools for the genetic manipulation of different malaria parasites for research purposes.

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