Dr Katharina Boroviak
Senior Staff Scientist
My interest and expertise lies in genome engineering and my research seeks to improve efficiency and accuracy of genome engineering tools, such as CRISPR/Cas9.
After my PhD in the laboratory of Dr. Henry Roehl at the University of Sheffield working on bone development in zebrafish, I took up a position as the Facility Manager of the Advanced Vector Design and BAC Recombineering Facility at the Wellcome Trust – Medical Research Council Stem Cell Institute (WT-MRC-SCI) in Cambridge. There we utilised BAC recombineering, Transcription activator-like effector nucleases (TALENs) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) to generate custom made targeting constructs for different faculty projects. During my time there I developed a keen interest in genome engineering.
In 2013 I joined the laboratory of Prof. Allan Bradley to work on CRISPR/Cas9 mediated genome engineering in mouse zygotes. I optimized the method in order to generate mouse models in the most efficient and effective manner. I also worked on determining the limitations of the CRISPR/Cas9 system and established protocols to generate models carrying indels (small insertions or deletions), point mutations (SNPs), genomic rearrangements (deletions, inversions, duplications) or Knock-Ins.
In 2016 I started managing part of the Bespoke Mouse Genome Engineering Team. The aim of this team is to generate mutant mouse lines for the research community. The team uses CRISPR/Cas9 in mouse zygotes to generate critical exon deletions and introduce point mutations (SNPs). CRISPR/Cas9 is also used in mouse embryonic stem cells (mESC) to generate more complex alleles such as knock-in of larger constructs (LacZ, GFP, COIN modules).
I took up the position of team leader of PlasmoGEM in 2018. I am hoping to put my expertise in genome engineering to good use in generating freely available tools for the genetic manipulation of different malaria parasites for research purposes.
Started as Team Leader of PlasmoGEM, Wellcome Sanger Institute, Cambridge, UK
Started as Staff Scientist managing part of the Bespoke Mouse Genome Engineering Team, Wellcome Sanger Institute, Cambridge, UK
Started as Staff Scientist in Prof. Allan Bradleys lab at the Wellcome Sanger Institute, Cambridge, UK
Started as Facility Manager of the Advanced Vector Design and BAC Recombineering Facility at the Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge, UK
PhD in Biomedical Science, Univ. of Sheffield, UK
PhD student in the laboratory of Dr. Henry Roehl, Univ. of Sheffield, UK
Master in Molecular Biology, Univ. of Vienna, Austria
Undergraduate study in the laboratory of Dr. Werner Zwerschke, TKFI, Austria