The Yusa laboratory focuses on the development of genome engineering technologies that allow us to perform unbiased genome-wide genetic screens in mammalian cells. We have recently adopted the CRISPR-Cas9 technology as a screening tool and generated genome-scale lentiviral CRISPR libraries. The current focus of the laboratory is further development and improvement of the CRISPR screening methodology and application of the screening technology in stem cell and oncology research.
Stem cell biology: Maintenance of and exit from pluripotency in mouse and human ES/iPS cells. Differentiation. Lineage reprogramming.
Oncology: Genetic vulnerabilities in cancer cells. The mechanisms of drug resistance. Sensitisation to targeted therapies.