We are part of the wider Illumina sequencing team that generates data for all of the faculty research programmes at the Wellcome Sanger Institute. In the Bespoke team, we use our resources to support samples that cannot be handled by processes in the high throughput teams within DNA Pipelines Operations. This might be due to insufficient DNA or RNA, poor sample performance within standard processes or a requirement for a novel library type. In this way, we devote more resources per sample in order to generate the sequencing data at the required quality. Currently we offer the following services: 10X Genomics Single Cell and Spatial Transcriptomics (Visium) library preparation, ChIPseq, RNAseq, Small RNAseq, DAFT-seq, TraDIS, WGS (including PCR-free options), scATAC-seq, qPCR & custom library creation.