Fluorescence in situ hybridisation (FISH) is a laboratory technique for detecting and locating specific DNA sequences on metaphase chromosomes and interphase nuclei. The technique relies on exposing the chromosome to a small DNA sequence called a probe that has a fluorescent molecule attached to it. The probe will only anneal to the complementary sequences under optimised condition. Simultaneous hybrisation of multiple probes, each labelled with a different fluorescent dye or a different combination of multiple dyes, allows multiple targets in a single specimen to be visualised in one hybridisation. FISH thus enables the link between genomics and cyotgeneitcs.
Nearly all our services rely on FISH, including physical mapping by metaphase-, interphase- and fibre-FISH; karyotyping of cancer and reprogrammed cell lines by Multiplex-FISH, studying the evolutionary chromosome rearrangement by cross-species chromosome painting etc.
We study variation in the DNA of people from different parts of the world, and also in related species such as chimpanzees and gorillas. This tells us about the evolutionary history of human populations and also allows us to compare the different species.
Integration of molecular cytogenetics, dated molecular phylogeny, and model-based predictions to understand the extreme chromosome reorganization in the Neotropical genus Tonatia (Chiroptera: Phyllostomidae).
Sotero-Caio CG, Volleth M, Hoffmann FG, Scott L, Wichman HAet al.
Phylogenetic reconstruction by cross-species chromosome painting and G-banding in four species of Phyllostomini tribe (Chiroptera, Phyllostomidae) in the Brazilian Amazon: an independent evidence for monophyly.
A phylogenetic analysis using multidirectional chromosome painting of three species (Uroderma magnirostrum, U. bilobatum and Artibeus obscurus) of subfamily Stenodermatinae (Chiroptera-Phyllostomidae).
Avian comparative genomics: reciprocal chromosome painting between domestic chicken (Gallus gallus) and the stone curlew (Burhinus oedicnemus, Charadriiformes)--an atypical species with low diploid number.
Nie W, O'Brien PC, Ng BL, Fu B, Volobouev Vet al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2009;17;1;99-113
Comparative genome maps of the pangolin, hedgehog, sloth, anteater and human revealed by cross-species chromosome painting: further insight into the ancestral karyotype and genome evolution of eutherian mammals.
Yang F, Graphodatsky AS, Li T, Fu B, Dobigny Get al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2006;14;3;283-96
Comparative chromosome painting between the domestic pig (Sus scrofa) and two species of peccary, the collared peccary (Tayassu tajacu) and the white-lipped peccary (T. pecari): a phylogenetic perspective.
Bosma AA, de Haan NA, Arkesteijn GJ, Yang F, Yerle M and Zijlstra C
Use of flow-sorted canine chromosomes in the assignment of canine linkage, radiation hybrid, and syntenic groups to chromosomes: refinement and verification of the comparative chromosome map for dog and human.
Sargan DR, Yang F, Squire M, Milne BS, O'Brien PC and Ferguson-Smith MA