Fluorescence in situ hybridisation (FISH) is a laboratory technique for detecting and locating specific DNA sequences on metaphase chromosomes and interphase nuclei. The technique relies on exposing the chromosome to a small DNA sequence called a probe that has a fluorescent molecule attached to it. The probe will only anneal to the complementary sequences under optimised condition. Simultaneous hybrisation of multiple probes, each labelled with a different fluorescent dye or a different combination of multiple dyes, allows multiple targets in a single specimen to be visualised in one hybridisation. FISH thus enables the link between genomics and cyotgeneitcs.
Nearly all our services rely on FISH, including physical mapping by metaphase-, interphase- and fibre-FISH; karyotyping of cancer and reprogrammed cell lines by Multiplex-FISH, studying the evolutionary chromosome rearrangement by cross-species chromosome painting etc.
Integration of molecular cytogenetics, dated molecular phylogeny, and model-based predictions to understand the extreme chromosome reorganization in the Neotropical genus Tonatia (Chiroptera: Phyllostomidae).
Sotero-Caio CG, Volleth M, Hoffmann FG, Scott L, Wichman HAet al.
Phylogenetic reconstruction by cross-species chromosome painting and G-banding in four species of Phyllostomini tribe (Chiroptera, Phyllostomidae) in the Brazilian Amazon: an independent evidence for monophyly.
A phylogenetic analysis using multidirectional chromosome painting of three species (Uroderma magnirostrum, U. bilobatum and Artibeus obscurus) of subfamily Stenodermatinae (Chiroptera-Phyllostomidae).
Avian comparative genomics: reciprocal chromosome painting between domestic chicken (Gallus gallus) and the stone curlew (Burhinus oedicnemus, Charadriiformes)--an atypical species with low diploid number.
Nie W, O'Brien PC, Ng BL, Fu B, Volobouev Vet al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2009;17;1;99-113
Comparative genome maps of the pangolin, hedgehog, sloth, anteater and human revealed by cross-species chromosome painting: further insight into the ancestral karyotype and genome evolution of eutherian mammals.
Yang F, Graphodatsky AS, Li T, Fu B, Dobigny Get al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2006;14;3;283-96
Comparative chromosome painting between the domestic pig (Sus scrofa) and two species of peccary, the collared peccary (Tayassu tajacu) and the white-lipped peccary (T. pecari): a phylogenetic perspective.
Bosma AA, de Haan NA, Arkesteijn GJ, Yang F, Yerle M and Zijlstra C
Use of flow-sorted canine chromosomes in the assignment of canine linkage, radiation hybrid, and syntenic groups to chromosomes: refinement and verification of the comparative chromosome map for dog and human.
Sargan DR, Yang F, Squire M, Milne BS, O'Brien PC and Ferguson-Smith MA