Archive Page: Liu Group | Mouse cancer genetics

Archive Page: Liu Group | Mouse cancer genetics

Liu Group

Pentao Liu left the Wellcome Sanger Institute in January 2018 to become Professor of Biomedical Sciences in the Li Ka Shing Faculty of Medicine and Stem Cell Centre at the University of Hong Kong. This page is no longer being updated and is maintained as historical record of the group's research at the Sanger Institute.
liu-group-9.jpgSanger Institute, Genome Research Limited

Our Research and Approach

We use various approaches including genetics, genomics and cell biology to study gene functions in normal development and disease such as cancer. We are particularly interested in stem cell self-renewal, differentiation, and lineage choice.


Dr Pentao Liu
Group Leader

We used various approaches including genetics, genomics and cell biology to study gene functions in normal development and disease such as cancer. We were particularly interested in stem cell self-renewal, differentiation, and lineage choice.

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  • A new patient-derived iPSC model for dystroglycanopathies validates a compound that increases glycosylation of α-dystroglycan.

    Kim J, Lana B, Torelli S, Ryan D, Catapano F et al.

    EMBO reports 2019;20;11;e47967

  • Expanded potential stem cell media as a tool to study human developmental hematopoiesis in vitro.

    Wilkinson AC, Ryan DJ, Kucinski I, Wang W, Yang J et al.

    Experimental hematology 2019;76;1-12.e5

  • Establishment of porcine and human expanded potential stem cells.

    Gao X, Nowak-Imialek M, Chen X, Chen D, Herrmann D et al.

    Nature cell biology 2019;21;6;687-699

  • Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming.

    Yang J, Rajan SS, Friedrich MJ, Lan G, Zou X et al.

    Stem cell reports 2019;12;4;757-771

  • Functional characterization of common BCL11B gene desert variants suggests a lymphocyte-mediated association of BCL11B with aortic stiffness.

    Maskari RA, Hardege I, Cleary S, Figg N, Li Y et al.

    European journal of human genetics : EJHG 2018;26;11;1648-1657

  • Toll-like receptor 2 costimulation potentiates the antitumor efficacy of CAR T Cells.

    Lai Y, Weng J, Wei X, Qin L, Lai P et al.

    Leukemia 2018;32;3;801-808

  • Transformation of Accessible Chromatin and 3D Nucleome Underlies Lineage Commitment of Early T Cells.

    Hu G, Cui K, Fang D, Hirose S, Wang X et al.

    Immunity 2018;48;2;227-242.e8

  • Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing.

    Wang K, Jin Q, Ruan D, Yang Y, Liu Q et al.

    Genome research 2017;27;12;2061-2071

  • Characterization of the single-cell derived bovine induced pluripotent stem cells.

    Zhao L, Wang Z, Zhang J, Yang J, Gao X et al.

    Tissue & cell 2017;49;5;521-527

  • CRISPR/Cas9-Mediated Deletion of Foxn1 in NOD/SCID/IL2rg-/- Mice Results in Severe Immunodeficiency.

    Wei X, Lai Y, Li B, Qin L, Xu Y et al.

    Scientific reports 2017;7;1;7720

  • Incorporation of a hinge domain improves the expansion of chimeric antigen receptor T cells.

    Qin L, Lai Y, Zhao R, Wei X, Weng J et al.

    Journal of hematology & oncology 2017;10;1;68

  • Genome-wide transposon screening and quantitative insertion site sequencing for cancer gene discovery in mice.

    Friedrich MJ, Rad L, Bronner IF, Strong A, Wang W et al.

    Nature protocols 2017;12;2;289-309

  • Visualization and Quantification of Browning Using a Ucp1-2A-Luciferase Knock-in Mouse Model.

    Mao L, Nie B, Nie T, Hui X, Gao X et al.

    Diabetes 2017;66;2;407-417

  • CD215+ Myeloid Cells Respond to Interleukin 15 Stimulation and Promote Tumor Progression.

    Lin S, Huang G, Xiao Y, Sun W, Jiang Y et al.

    Frontiers in immunology 2017;8;1713

  • Cell lineage specification at single cell resolution.

    Yang J and Liu P

    Stem cell investigation 2017;4;76

  • PSCA and MUC1 in non-small-cell lung cancer as targets of chimeric antigen receptor T cells.

    Wei X, Lai Y, Li J, Qin L, Xu Y et al.

    Oncoimmunology 2017;6;3;e1284722

  • Single-cell RNA-seq identifies a PD-1hi ILC progenitor and defines its development pathway.

    Yu Y, Tsang JC, Wang C, Clare S, Wang J et al.

    Nature 2016;539;7627;102-106

  • Harmine Induces Adipocyte Thermogenesis through RAC1-MEK-ERK-CHD4 Axis.

    Nie T, Hui X, Mao L, Nie B, Li K et al.

    Scientific reports 2016;6;36382

  • Heterogeneity of CD34 and CD38 expression in acute B lymphoblastic leukemia cells is reversible and not hierarchically organized.

    Jiang Z, Deng M, Wei X, Ye W, Xiao Y et al.

    Journal of hematology & oncology 2016;9;1;94

  • Structure-function integrity of the adult hippocampus depends on the transcription factor Bcl11b/Ctip2.

    Simon R, Baumann L, Fischer J, Seigfried FA, De Bruyckere E et al.

    Genes, brain, and behavior 2016;15;4;405-19

  • MLL1: the thin red line divides naïve and primed pluripotency.

    Yang J and Liu P

    Stem cell investigation 2016;3;63

  • CRISPR/Cas9 somatic multiplex-mutagenesis for high-throughput functional cancer genomics in mice.

    Weber J, Öllinger R, Friedrich M, Ehmer U, Barenboim M et al.

    Proceedings of the National Academy of Sciences of the United States of America 2015;112;45;13982-7

  • Single Cell RNA-Sequencing of Pluripotent States Unlocks Modular Transcriptional Variation.

    Kolodziejczyk AA, Kim JK, Tsang JC, Ilicic T, Henriksson J et al.

    Cell stem cell 2015;17;4;471-85

  • Single-cell transcriptomic reconstruction reveals cell cycle and multi-lineage differentiation defects in Bcl11a-deficient hematopoietic stem cells.

    Tsang JC, Yu Y, Burke S, Buettner F, Wang C et al.

    Genome biology 2015;16;178

  • Bcl11a (Ctip1) Controls Migration of Cortical Projection Neurons through Regulation of Sema3c.

    Wiegreffe C, Simon R, Peschkes K, Kling C, Strehle M et al.

    Neuron 2015;87;2;311-25

  • The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development.

    Yu Y, Wang C, Clare S, Wang J, Lee SC et al.

    The Journal of experimental medicine 2015;212;6;865-74

  • Bcl11b is essential for group 2 innate lymphoid cell development.

    Walker JA, Oliphant CJ, Englezakis A, Yu Y, Clare S et al.

    The Journal of experimental medicine 2015;212;6;875-82

  • Quantitative evaluation of the immunodeficiency of a mouse strain by tumor engraftments.

    Ye W, Jiang Z, Li GX, Xiao Y, Lin S et al.

    Journal of hematology & oncology 2015;8;59

  • ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells.

    Xiao Y, Jiang Z, Li Y, Ye W, Jia B et al.

    Haematologica 2015;100;5;585-94

  • Conversion of non-adipogenic fibroblasts into adipocytes by a defined hormone mixture.

    Nie T, Hui X, Gao X, Nie B, Mao L et al.

    The Biochemical journal 2015;467;3;487-94

  • Signalling Through Retinoic Acid Receptors is Required for Reprogramming of Both Mouse Embryonic Fibroblast Cells and Epiblast Stem Cells to Induced Pluripotent Stem Cells.

    Yang J, Wang W, Ooi J, Campos LS, Lu L and Liu P

    Stem cells (Dayton, Ohio) 2015;33;5;1390-404

  • Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes.

    Li W, Jiang Z, Li T, Wei X, Zheng Y et al.

    Molecular cancer 2015;14;26

  • BCL11A is a triple-negative breast cancer gene with critical functions in stem and progenitor cells.

    Khaled WT, Choon Lee S, Stingl J, Chen X, Raza Ali H et al.

    Nature communications 2015;6;5987

  • A conditional piggyBac transposition system for genetic screening in mice identifies oncogenic networks in pancreatic cancer.

    Rad R, Rad L, Wang W, Strong A, Ponstingl H et al.

    Nature genetics 2015;47;1;47-56

  • A marker-free system for highly efficient construction of vaccinia virus vectors using CRISPR Cas9.

    Yuan M, Gao X, Chard LS, Ali Z, Ahmed J et al.

    Molecular therapy. Methods & clinical development 2015;2;15035

  • Points to consider in the development of seed stocks of pluripotent stem cells for clinical applications: International Stem Cell Banking Initiative (ISCBI).

    Andrews PW, Baker D, Benvinisty N, Miranda B, Bruce K et al.

    Regenerative medicine 2015;10;2 Suppl;1-44

  • Comparison of TALE designer transcription factors and the CRISPR/dCas9 in regulation of gene expression by targeting enhancers.

    Gao X, Tsang JC, Gaba F, Wu D, Lu L and Liu P

    Nucleic acids research 2014;42;20;e155

  • Cellular reprogramming by transcription factor engineering.

    Tsang JC, Gao X, Lu L and Liu P

    Current opinion in genetics & development 2014;28;1-9

  • Single-cell analyses of regulatory network perturbations using enhancer-targeting TALEs suggest novel roles for PU.1 during haematopoietic specification.

    Wilkinson AC, Kawata VK, Schütte J, Gao X, Antoniou S et al.

    Development (Cambridge, England) 2014;141;20;4018-30

  • Polygenic in vivo validation of cancer mutations using transposons.

    Chew SK, Lu D, Campos LS, Scott KL, Saci A et al.

    Genome biology 2014;15;9;455

  • JAK2V617F homozygosity drives a phenotypic switch in myeloproliferative neoplasms, but is insufficient to sustain disease.

    Li J, Kent DG, Godfrey AL, Manning H, Nangalia J et al.

    Blood 2014;123;20;3139-51

  • Cancer mouse models: past, present and future.

    Khaled WT and Liu P

    Seminars in cell & developmental biology 2014;27;54-60

  • Absence of Appl2 sensitizes endotoxin shock through activation of PI3K/Akt pathway.

    Mao L, Lin W, Nie T, Hui X, Gao X et al.

    Cell & bioscience 2014;4;1;60

  • Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo.

    Wu X, Satpathy AT, Kc W, Liu P, Murphy TL and Murphy KM

    PloS one 2013;8;5;e64800

  • Enhanced production of recombinant secretory proteins in Pichia pastoris by optimizing Kex2 P1' site.

    Yang S, Kuang Y, Li H, Liu Y, Hui X et al.

    PloS one 2013;8;9;e75347

  • Reprogramming to pluripotency using designer TALE transcription factors targeting enhancers.

    Gao X, Yang J, Tsang JC, Ooi J, Wu D and Liu P

    Stem cell reports 2013;1;2;183-97

  • Bcl11a is essential for lymphoid development and negatively regulates p53.

    Yu Y, Wang J, Khaled W, Burke S, Li P et al.

    The Journal of experimental medicine 2012;209;13;2467-83

  • Bcl11a is required for neuronal morphogenesis and sensory circuit formation in dorsal spinal cord development.

    John A, Brylka H, Wiegreffe C, Simon R, Liu P et al.

    Development (Cambridge, England) 2012;139;10;1831-41

  • Large-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell lines.

    Davis MP, Abreu-Goodger C, van Dongen S, Lu D, Tate PH et al.

    PloS one 2012;7;8;e41762

  • Pluripotency and its layers of complexity.

    Ooi J and Liu P

    Cell regeneration (London, England) 2012;1;1;7

  • Using mouse models to study function of transcriptional factors in T cell development.

    Li P, Xiao Y, Liu Z and Liu P

    Cell regeneration (London, England) 2012;1;1;8

  • Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1.

    Wang W, Yang J, Liu H, Lu D, Chen X et al.

    Proceedings of the National Academy of Sciences of the United States of America 2011;108;45;18283-8

  • Critical role of Bcl11b in suppressor function of T regulatory cells and prevention of inflammatory bowel disease.

    Vanvalkenburgh J, Albu DI, Bapanpally C, Casanova S, Califano D et al.

    The Journal of experimental medicine 2011;208;10;2069-81

  • Stella-Cre mice are highly efficient Cre deleters.

    Liu H, Wang W, Chew SK, Lee SC, Li J et al.

    Genesis (New York, N.Y. : 2000) 2011;49;8;689-95

  • Carnitine palmitoyltransferase 1A prevents fatty acid-induced adipocyte dysfunction through suppression of c-Jun N-terminal kinase.

    Gao X, Li K, Hui X, Kong X, Sweeney G et al.

    The Biochemical journal 2011;435;3;723-32

  • Transcription factor Bcl11b controls selection of invariant natural killer T-cells by regulating glycolipid presentation in double-positive thymocytes.

    Albu DI, VanValkenburgh J, Morin N, Califano D, Jenkins NA et al.

    Proceedings of the National Academy of Sciences of the United States of America 2011;108;15;6211-6

  • Genetic screens using the piggyBac transposon.

    Chew SK, Rad R, Futreal PA, Bradley A and Liu P

    Methods (San Diego, Calif.) 2011;53;4;366-71

  • LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease.

    Griffiths DS, Li J, Dawson MA, Trotter MW, Cheng YH et al.

    Nature cell biology 2011;13;1;13-21

  • PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice.

    Rad R, Rad L, Wang W, Cadinanos J, Vassiliou G et al.

    Science (New York, N.Y.) 2010;330;6007;1104-7

  • Critical roles of Bcl11b in T-cell development and maintenance of T-cell identity.

    Liu P, Li P and Burke S

    Immunological reviews 2010;238;1;138-49

  • JAK2 V617F impairs hematopoietic stem cell function in a conditional knock-in mouse model of JAK2 V617F-positive essential thrombocythemia.

    Li J, Spensberger D, Ahn JS, Anand S, Beer PA et al.

    Blood 2010;116;9;1528-38

  • Antigen-specific clonal expansion and cytolytic effector function of CD8+ T lymphocytes depend on the transcription factor Bcl11b.

    Zhang S, Rozell M, Verma RK, Albu DI, Califano D et al.

    The Journal of experimental medicine 2010;207;8;1687-99

  • The Merlin/NF2 tumor suppressor functions through the YAP oncoprotein to regulate tissue homeostasis in mammals.

    Zhang N, Bai H, David KK, Dong J, Zheng Y et al.

    Developmental cell 2010;19;1;27-38

  • Reprogramming of T cells to natural killer-like cells upon Bcl11b deletion.

    Li P, Burke S, Wang J, Chen X, Ortiz M et al.

    Science (New York, N.Y.) 2010;329;5987;85-9

  • A modified sleeping beauty transposon system that can be used to model a wide variety of human cancers in mice.

    Dupuy AJ, Rogers LM, Kim J, Nannapaneni K, Starr TK et al.

    Cancer research 2009;69;20;8150-6

  • Construction of gene-targeting vectors by recombineering.

    Lee SC and Liu P

    Cold Spring Harbor protocols 2009;2009;9;pdb.prot5291

  • piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.

    Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M et al.

    Nature 2009;458;7239;766-70

  • A DNA transposon-based approach to validate oncogenic mutations in the mouse.

    Su Q, Prosser HM, Campos LS, Ortiz M, Nakamura T et al.

    Proceedings of the National Academy of Sciences of the United States of America 2008;105;50;19904-9

  • Advantages of q-PCR as a method of screening for gene targeting in mammalian cells using conventional and whole BAC-based constructs.

    Gómez-Rodríguez J, Washington V, Cheng J, Dutra A, Pak E et al.

    Nucleic acids research 2008;36;18;e117

  • Mouse embryonic stem cell-based functional assay to evaluate mutations in BRCA2.

    Kuznetsov SG, Liu P and Sharan SK

    Nature medicine 2008;14;8;875-81

  • Chromosomal transposition of PiggyBac in mouse embryonic stem cells.

    Wang W, Lin C, Lu D, Ning Z, Cox T et al.

    Proceedings of the National Academy of Sciences of the United States of America 2008;105;27;9290-5

  • BCL11B is required for positive selection and survival of double-positive thymocytes.

    Albu DI, Feng D, Bhattacharya D, Jenkins NA, Copeland NG et al.

    The Journal of experimental medicine 2007;204;12;3003-15

  • A recombineering based approach for high-throughput conditional knockout targeting vector construction.

    Chan W, Costantino N, Li R, Lee SC, Su Q et al.

    Nucleic acids research 2007;35;8;e64

  • A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction.

    Adams DJ, Quail MA, Cox T, van der Weyden L, Gorick BD et al.

    Genomics 2005;86;6;753-8

  • Inducible gene trapping with drug-selectable markers and Cre/loxP to identify developmentally regulated genes.

    Chen YT, Liu P and Bradley A

    Molecular and cellular biology 2004;24;22;9930-41

  • Bcl11a is essential for normal lymphoid development.

    Liu P, Keller JR, Ortiz M, Tessarollo L, Rachel RA et al.

    Nature immunology 2003;4;6;525-32

  • A highly efficient recombineering-based method for generating conditional knockout mutations.

    Liu P, Jenkins NA and Copeland NG

    Genome research 2003;13;3;476-84

  • Evi3, a common retroviral integration site in murine B-cell lymphoma, encodes an EBFAZ-related Krüppel-like zinc finger protein.

    Warming S, Liu P, Suzuki T, Akagi K, Lindtner S et al.

    Blood 2003;101;5;1934-40

  • Efficient Cre-loxP-induced mitotic recombination in mouse embryonic stem cells.

    Liu P, Jenkins NA and Copeland NG

    Nature genetics 2002;30;1;66-72