Dr Katharina Boroviak | Senior Staff Scientist

Boroviak, Katharina

My interest and expertise lies in genome engineering and my research seeks to improve efficiency and accuracy of genome engineering tools, such as CRISPR/Cas9.

After my PhD in the laboratory of Dr. Henry Roehl at the University of Sheffield working on bone development in zebrafish, I took up a position as the Facility Manager of the Advanced Vector Design and BAC Recombineering Facility at the Wellcome Trust - Medical Research Council Stem Cell Institute (WT-MRC-SCI) in Cambridge. There we utilised BAC recombineering, Transcription activator-like effector nucleases (TALENs) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) to generate custom made targeting constructs for different faculty projects. During my time there I developed a keen interest in genome engineering.

In 2013 I joined the laboratory of Prof. Allan Bradley to work on CRISPR/Cas9 mediated genome engineering in mouse zygotes. I optimized the method in order to generate mouse models in the most efficient and effective manner. I also worked on determining the limitations of the CRISPR/Cas9 system and established protocols to generate models carrying indels (small insertions or deletions), point mutations (SNPs), genomic rearrangements (deletions, inversions, duplications) or Knock-Ins.

In 2016 I started managing part of the Bespoke Mouse Genome Engineering Team. The aim of this team is to generate mutant mouse lines for the research community. The team uses CRISPR/Cas9 in mouse zygotes to generate critical exon deletions and introduce point mutations (SNPs). CRISPR/Cas9 is also used in mouse embryonic stem cells (mESC) to generate more complex alleles such as knock-in of larger constructs (LacZ, GFP, COIN modules).

I took up the position of team leader of PlasmoGEM in 2018. I am hoping to put my expertise in genome engineering to good use in generating freely available tools for the genetic manipulation of different malaria parasites for research purposes.

Publications

  • No unexpected CRISPR-Cas9 off-target activity revealed by trio sequencing of gene-edited mice.

    Iyer V, Boroviak K, Thomas M, Doe B, Riva L et al.

    PLoS genetics 2018;14;7;e1007503

  • Revealing hidden complexities of genomic rearrangements generated with Cas9.

    Boroviak K, Fu B, Yang F, Doe B and Bradley A

    Scientific reports 2017;7;1;12867

  • Chromosome engineering in zygotes with CRISPR/Cas9.

    Boroviak K, Doe B, Banerjee R, Yang F and Bradley A

    Genesis (New York, N.Y. : 2000) 2016;54;2;78-85

  • No unexpected CRISPR-Cas9 off-target activity revealed by trio sequencing of gene-edited mice.

    Iyer V, Boroviak K, Thomas M, Doe B, Riva L et al.

    PLoS genetics 2018;14;7;e1007503

  • WD40-repeat 47, a microtubule-associated protein, is essential for brain development and autophagy.

    Kannan M, Bayam E, Wagner C, Rinaldi B, Kretz PF et al.

    Proceedings of the National Academy of Sciences of the United States of America 2017;114;44;E9308-E9317

  • Revealing hidden complexities of genomic rearrangements generated with Cas9.

    Boroviak K, Fu B, Yang F, Doe B and Bradley A

    Scientific reports 2017;7;1;12867

  • Synthetic lethality between PAXX and XLF in mammalian development.

    Balmus G, Barros AC, Wijnhoven PW, Lescale C, Hasse HL et al.

    Genes & development 2016;30;19;2152-2157

  • C13orf31 (FAMIN) is a central regulator of immunometabolic function.

    Cader MZ, Boroviak K, Zhang Q, Assadi G, Kempster SL et al.

    Nature immunology 2016;17;9;1046-56

  • Chromosome engineering in zygotes with CRISPR/Cas9.

    Boroviak K, Doe B, Banerjee R, Yang F and Bradley A

    Genesis (New York, N.Y. : 2000) 2016;54;2;78-85

  • Expression of osterix Is Regulated by FGF and Wnt/β-Catenin Signalling during Osteoblast Differentiation.

    Felber K, Elks PM, Lecca M and Roehl HH

    PloS one 2015;10;12;e0144982

  • Hedgehog signalling is required for perichondral osteoblast differentiation in zebrafish.

    Felber K, Croucher P and Roehl HH

    Mechanisms of development 2011;128;1-2;141-52

  • Tracking gene expression during zebrafish osteoblast differentiation.

    Li N, Felber K, Elks P, Croucher P and Roehl HH

    Developmental dynamics : an official publication of the American Association of Anatomists 2009;238;2;459-66

  • Association of a functional polymorphism in the clopidogrel target receptor gene, P2Y12, and the risk for ischemic cerebrovascular events in patients with peripheral artery disease.

    Ziegler S, Schillinger M, Funk M, Felber K, Exner M et al.

    Stroke 2005;36;7;1394-9

Boroviak, Katharina
Katharina's Timeline
2018

Started as Team Leader of PlasmoGEM, Wellcome Sanger Institute, Cambridge, UK

2016

Started as Staff Scientist managing part of the Bespoke Mouse Genome Engineering Team, Wellcome Sanger Institute, Cambridge, UK

2013

Started as Staff Scientist in Prof. Allan Bradleys lab at the Wellcome Sanger Institute, Cambridge, UK

2010

Started as Facility Manager of the Advanced Vector Design and BAC Recombineering Facility at the Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge, UK

PhD in Biomedical Science, Univ. of Sheffield, UK

2007

PhD student in the laboratory of Dr. Henry Roehl, Univ. of Sheffield, UK

Master in Molecular Biology, Univ. of Vienna, Austria

2006

Undergraduate study in the laboratory of Dr. Werner Zwerschke, TKFI, Austria