Mouse Genome Engineering

Model Pipelines

Archive Page

This page is maintained as a historical record and is no longer being updated.

Model Pipelines produced and characterised knock-out mice for large-scale research projects, including the Sanger Mouse Genetics Project, National Institutes of Health KOMP2, EUCOMMTools, and the Infrafrontier projects.

Mouse Pipelines included the following teams:

  • Mouse Genome Engineering
  • Mouse Transgenic Technologies
  • Mouse Molecular Technologies
  • Mouse Phenotyping

In addition the team was closely involved in three major Strategic Award projects funded by the Wellcome Trust:

  • Deciphering the Mechanisms of Developmental Disorders (DMDD)
  • Origins of Bone and Cartilage Disease (OBCD)
  • Immune function and pathology dissected by high-throughput analysis of mice with targeted gene disruptions – an investigation by the Infection and Immunity Immunophenotyping (3i) consortium.

We served the scientific community with genetically altered strains of mice by supplying public repositories and researchers directly. Availability of these mouse strains was complemented by the standardised phenotypic characterisation performed by Mouse Pipelines, that was made freely available to the scientific community. Accordingly, the research community continues to produce a growing scientific output using the resources distributed from the Mouse Pipelines at the Institute. To support this important part of our mission, we had an office to carry out cost-recovery.

The team researched and developed mouse resource production methods. For example we implemented the use of the CRISPR mouse mutagenesis technology directly in mouse embryos, which led to great savings and acceleration of projects in their early phase by removing the need to use embryonic stem cells. We worked flexibly so that we could rapidly shift our resources to advance all large-scale projects as needed while also supporting the needs of individual Faculty members.

We worked closely with the Mouse Informatics and Research Support Facility teams to develop the Mouse Database. This work was essential for the welfare of our mice, to maintain optimal operational workflows, and to achieve our scientific goals, as well as for streamlining publication of our scientific results through the International Mouse Phenotyping Consortium (IMPC) web portal.