Wellcome Sanger Institute

Molecular Cytogenetics

Scientific Operations

Fluorescence in situ hybridisation (FISH) is a laboratory technique for detecting and locating specific DNA sequences on metaphase chromosomes and interphase nuclei. The technique relies on exposing the chromosome to a small DNA sequence called a probe that has a fluorescent molecule attached to it. The probe will only anneal to the complementary sequences under optimised condition. Simultaneous hybrisation of multiple probes, each labelled with a different fluorescent dye or a different combination of multiple dyes, allows multiple targets in a single specimen to be visualised in one hybridisation. FISH thus enables the link between genomics and cyotgeneitcs.

Nearly all our services rely on FISH, including physical mapping by metaphase-, interphase- and fibre-FISH; karyotyping of cancer and reprogrammed cell lines by Multiplex-FISH, studying the evolutionary chromosome rearrangement by cross-species chromosome painting etc.

Currently we can provide the following FISH services:

1. Physical assigment of BACs, fosmids, transgenes, cDNA clones onto metaphase chromosomes.

2. High-resolution mapping and gap sizing by fibre-FISH with single-molecule DNA fibres and extended chromomatin fibres.

3. Karyotyping by combined M-FISH and inverted DAPI-banding.

4. Validation of structural and copy number variations.

5. Multi-directional chromosome painting

Please submit your requests via: fish-requests@sanger.ac.uk

Core team

Photo of Ms Beiyuan Fu

Ms Beiyuan Fu

Senior Research Assistant

Photo of Dr Ruby Banerjee

Dr Ruby Banerjee

Senior Research Assistant

Previous team members

Photo of Sandra Louzada Gomes Pereira

Sandra Louzada Gomes Pereira

Senior Research Assistant

 

Publications

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