Cytometry Core Facility
Scientific Operations
For a more in-depth explanation, listing of services and common flow cytometry applications, click read more.
Cell Analysers with High Throughput Sampler function
The facility has 4 high-throughput flow cytometer analysers with plate-reading capacity. These are all user-operated after user training by core facility staff.
- BD LSRII with HTS
- BD LSRFortessa1 with HTS
- BD LSRFortessa2 with HTS
- Beckman Coulter CytoFlex S
High Speed cell Sorters
The facility has 5 high speed flow cytometer sorters. 4 of them are housed in a Class ll Microbiological Safety Cabinets. The sorters are operated by core facility staff only except Sony SH800 and MA900.
- Mo-Flo (The Legacy)
- Mo-FloXDP
- BD INFLUX (Index cell sorting)
- 2 Sony Sorters (Index cell sorting)
Data Analysis Software
The Institute is currently running on a Site License by Flowjo, a flow cytometry data analysis software package which offers a range of analysis platforms that includes compensation, cell cycle, proliferation studies and kinetics. Activation of license is required for usage. Please contact the core facility at cytometry@sanger.ac.uk for more details.
Flow Cytometry Training
The facility offers analyser, sorter and analysis training for all users of the facility. Please visit iGrow
Booking
All flow cytometers are booked through the online booking calendar PPMS. For details on how to make booking, please visit Cytometry confluence page.
What is flow cytometry?
Flow Cytometry is a laser based technology that is used to measure the physical and chemical characteristics of cells. Such measurements are made while the cells flow in a liquid medium past an excitation light source and the emitted fluorescence captured by its measuring apparatus. It is an important tool for many scientific disciplines as it allows qualitative and quantitative examination of the whole cells as well as characterization of cells within a sample.
To learn more, please click this website.
Common Applications of Flow Cytometry
Core team

Jennie Graham
Senior Research Assistant

Sam Thompson
Research Assistant
Previous team members

Juneid Abdul-Karim
Advanced Research Assistant

Christopher Hall
Senior Research Assistant
Related groups
Programmes and Facilities
Partners
We work with the following groups
External
Amanda Fisher
External
Christine Harrison
External
Niels Morling
External
Matthew Breen
External
Vladimir Trifonov
External