Cytometry Core Facility
For a more in-depth explanation, listing of services and common flow cytometry applications, click read more.
Cell Analysers with High Throughput Sampler function
The facility has 4 high-throughput flow cytometer analysers with plate-reading capacity. These are all user-operated after user training by core facility staff.
- BD LSRII with HTS
- BD LSRFortessa1 with HTS
- BD LSRFortessa2 with HTS
- Beckman Coulter CytoFlex S
High Speed cell Sorters
The facility has 5 high speed flow cytometer sorters. 4 of them are housed in a Class ll Microbiological Safety Cabinets. The sorters are operated by core facility staff only except Sony SH800 and MA900.
- Mo-Flo (The Legacy)
- BD INFLUX (Index cell sorting)
- 2 Sony Sorters (Index cell sorting)
Data Analysis Software
The Institute is currently running on a Site License by Flowjo, a flow cytometry data analysis software package which offers a range of analysis platforms that includes compensation, cell cycle, proliferation studies and kinetics. Activation of license is required for usage. Please contact the core facility at firstname.lastname@example.org for more details.
Flow Cytometry Training
The facility offers analyser, sorter and analysis training for all users of the facility. Please visit iGrow
What is flow cytometry?
Flow Cytometry is a laser based technology that is used to measure the physical and chemical characteristics of cells. Such measurements are made while the cells flow in a liquid medium past an excitation light source and the emitted fluorescence captured by its measuring apparatus. It is an important tool for many scientific disciplines as it allows qualitative and quantitative examination of the whole cells as well as characterization of cells within a sample.
To learn more, please click this website.
Common Applications of Flow Cytometry
Bee Ling Ng
Senior Scientific Manager
The Cytometry Core Facility team is made up of 4 staff members. All of our team members have many years of experience in different aspects of flow cytometry. We dedicate our time and efforts in helping and assisting our researchers in their flow cytometry experiments. The facility collaborates with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing, microarrays as well as fluorescence in-situ hybridisation (FISH) experiment.
Experimental Cancer Genetics
We are a team of cancer biologists, geneticists and computational biologists interested in understanding how cancers develop and the ways of ...
Cancer Genome Project
Cancer Genetics & Genomics
Throughout life, the genome within cells of the human body is exposed to DNA damage and suffers mistakes in replication. These ...