Single-cell multi-omics tissue mapping and organoid-based disease modeling
1) Single-cell multi-omics sequencing
We will establish single-cell sequencing methods permitting the capture of multimodal information including epigenomics, transcriptomic and proteomics states from individual cells. We will build analysis pipelines to integrate multi-omics profiles and construct comprehensive cellular-state map in tissues.
2) CRISPR-perturbation screening
We will establish organoid models that recapitulate tissue development process. We will develop CRISPR libraries that target epigenetic regulators and perform pooled screening. We will design algorithms that enable the prediction of gene expression patterns base on specific epigenetic markers.
We seek to explore the vast cellular diversity in the human brain using large-scale spatial transcriptomics, imaging and functional screens.