Wellcome Sanger Institute

Bespoke Sequencing

Scientific Operations

The remit of the Bespoke team within DNA Pipelines Operations is to process samples that cannot be handled within the standard pipeline provided by the high throughput teams. This might be due to insufficient DNA, poor sample performance within standard processes or a requirement for a novel library type.

We provide our customers with access to products and processes that cannot be supported by the high throughput teams within DNA Pipelines Operations. The bespoke team offers low throughput custom processes that are tailored to specific projects. This is an important part of our strategy as we seek to provide the greatest range and scale of sequencing solutions across a range of sample types, species and library types.

Bespoke Team

In the Bespoke team, we use our resources to support samples that cannot be handled by processes in the high throughput team. This might be due to insufficient DNA or RNA, poor sample performance within standard processes or a requirement for a novel library type. In this way, we devote more resources per sample in order to generate the sequencing data at the required quality.

Our current library types include:

  • 10X Genomics single cell and Spatial Transcriptomics (Visium) library construction
  • scATAC-seq
  • qPCR
  • RNAseq (including rRNA depletion for bacterial samples)
  • Small RNAseq
  • ChIPseq
  • DAFT-seq
  • TraDIS
  • QIseq
  • WGS
  • HiC library construction
  • Custom Library Creation

This list is not exhaustive, we are always seeking to expand our portfolio of activities to meet the needs of our faculty customers.

Our people

Group lead

Core team

Photo of Matthew Mayho, PhD

Matthew Mayho, PhD

Staff Scientist