Platform for personalised CRISPR screening libraries

Patient and cell-type specific CRISPR screening libraries

Technology

The process of finding disease targets has been revolutionised by the technology known as CRISPR screening. However, current CRISPR screens ignore the genetic diversity present in patient derived disease models and uses off-the-shelf screening libraries.
This one-size-fits-all approach fails to detect more personalized disease targets. In addition, massive parallel oligo design and synthesis for whole genome CRISPR screens is resource intensive and costly.

This Personalised CRISPR platform can generate patient and cell-type specific CRISPR screening libraries in a high-throughput and cost-effective manner. It can identify targets both in the coding and non-coding regions of the genome.

This technology is:

• Personalised
• Scalable to any cell line and goes from genome-wide to specific regions
• It takes under one week (conventional CRISPR libraries take two to three months)
• Cost -effective: less than £100

Advantages

• Personalisation: Unlike conventional CRISPR libraries, our platform can be personalised with disease-specific genetic signatures.
• Scalable: It can be applied across various cell lines and species and can capture targets genome-wide or in specific regions.
• Fast: Reduces the time of CRISPR library synthesising down to one week, as opposed to two – three months with conventional CRISPR libraries.
• Accessible: Does not require prior knowledge of genomic sequence or a bioinformativian for designing the libraries.

Applications/Context

• Identification of novel targets missed by currently available CRISPR libraries.

Comparable Technologies

Other conventional CRISPR libraries are not personalised, are difficult to customise for different uses, take from two to three months and are less cost-effective.

Intellectual property

Patent filed on the technology.

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PERSONALISED PLATFORM FOR CRISPR SCREENING LIBRARIES_MAY 2022

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