Dr Nicole Muller-Sienerth
In her current role Nicole is responsible for several laboratory managing duties, including acting as local coordinator, supervising new starters, PhD students and contractors as well as ordering consumables and looking after equipment. Her work within the lab splits between her own research project, identifying binding partners for extracellular proteins, and providing recombinant proteins for collaborators within the Sanger Institute and outside.
As the lab manager I am responsible for the smooth running of the lab, including ordering consumables and equipment, initiating repairs, maintenance and servicing, performing local co-ordinator duties regarding Health and Safety, Permit to Work, Risk Assessments, New Starter Induction into CL2 and and their supervision. My current project involves cloning of soluble recombinant Fusion proteins, expression in mammalian cells and purification. I am working towards finding yet unknown binding partners to different types of extracellular proteins expressed on human platelets, the Malaria parasite Plasmodium falciparum etc. Using Flow Cytometry I analyse their binding to a wide range of cancer cell lines. Follow-up experiments will include Mass Spec analysis as well as using the CRISPR-Cas9 knock-out system.
The aim of my previouse project in this lab was to generate a panel of recombinant monoclonal antibodies against a library of Zebrafish cell surface proteins. This involved the expression and purification of extracellular domains of Zebrafish proteins, immunisation and hybridoma fusion, screening and cloning of recombinant monoclonal antibodies. These antibodies were validated via ELISA and immunohistology and made publicly available as plasmids on Addgene.
Laboratory Manager - Wellcome Trust Sanger Institute, Cambridge, UK
Advanced Research Assistant - Wellcome Trust Sanger Institute, Cambridge, UK: Generation of recombinant monoclonal antibodies against a library of Zebrafish cell surface proteins
PostDoc - University of Wuerzburg, Germany: Research in the field of Tumor Necrosis Factor (TNF) ligands and receptors
PhD in Biology - University of Wuerzburg, Germany: Dissertation title: ”Development of antigen-dependent activatable TNF ligand fusion proteins“
Master of Science (“Diplom”) in Biology - University of Hohenheim, Germany: Master Thesis (“Diplomarbeit”): “Expression, purification and molecular interaction of a cytoplasmic fragment from the receptor CD44 with Merlin”
Working student - Aventis Pharma Deutschland GmbH, Frankfurt/Main, Germany: Bacterial expression, purification and analysis of recombinant proteins