Dr Gabriel Rinaldi | Senior Staff Scientist

Rinaldi, Gabriel

Within the Berriman Group I supervise a functional genomic platform developed for blood flukes (schistosomes) that includes maintenance of the whole life cylcle of the parasite, collection and culture of all the developmental stages, and the use of genetic tools, including gene silencing and transgenesis coupled with highthrougput transcriptomic analyses and single-cell sequencing to study the role of parasite genes and their interactions with the hosts.

I am interested in understanding how parasites, in particular parasitic flatworms, infect, evade the immune response and establish an effective infection that allows them to survive for decades inside the hosts. Many of these parasites, responsible for the infection of hundred millions of people in the most impoverish regions of the world, are considered agents of Neglected Tropical Diseases by the WHO.

In particular, my interest in host-parasite interaction has been focused in the development of genetic approaches to manipulate the genome and/ or the expression of genes in order to elucidate gene function and reveal parasite vulnerabilities that would eventually lead to the development of novel control strategies by identifying targets for drugs and/ or vaccines.

We are currently applying novel approaches to manipulate the genome of these parasites, including transgenesis mediated by lentivirus and CRISPR-Cas in tandem with improved culture conditions of developmental stages, highthroughput transcriptomic analysis and single-cell sequencing.

My colleagues and I have undertaken extensive research during the past decade aimed at functional genomics of trematodes including blood flukes (schistosomes) and the liver flukes Fasciola hepatica and Opisthorchis viverrini. We have extensive experience with in vitro/ ex vivo culture of these pathogens, methods and approaches to transduce cultured schistosomes with retro/ lentivirus and other genome integration competent vectors including transposons, and mouse models for schistosomiasis mansoni and haematobia. I have been centrally involved in the development of gene silencing mediated by dsRNA (RNAi) in F. hepatica, and germ line transgenesis aiming to derive stable lines of schistosomes expressing reporters and/ or hairpin RNAs to drive vector-based RNAi.

I am also interest in the relationship between infection and cancer, more specifically between chronic infections with helminth parasites and carcinogensis. Colleagues and I have been involved in studies aiming to understand the role of the blood fluke Schistosoma haematobium in the genesis and progression of squamous cell carcinoma of the bladder, and the liver fluke Opisthorchis viverrini in cholangiocarcinoma (bile duct cancer).

More recently colleagues and I started to explore how helminth infections may affect the gut microbiome of the host. In particular, we have characterized quantitatively and qualitatively the fluctuations in the composition of the gut microbiome of mice experimentally infected with Schistosoma mansoni with the main goal of identifying microbial taxa with potential roles in the immune modulation and pathophysiology of the infection and disease progression.

Publications

  • Schistosoma mansoni infection is associated with quantitative and qualitative modifications of the mammalian intestinal microbiota.

    Jenkins TP, Peachey LE, Ajami NJ, MacDonald AS, Hsieh MH et al.

    Scientific reports 2018;8;1;12072

  • Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.

    Yan HB, Smout MJ, Ju C, Folley AE, Skinner DE et al.

    Antimicrobial agents and chemotherapy 2018;62;8

  • Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers.

    McNulty SN, Tort JF, Rinaldi G, Fischer K, Rosa BA et al.

    PLoS genetics 2017;13;1;e1006537

  • HIV-1 Integrates Widely throughout the Genome of the Human Blood Fluke Schistosoma mansoni.

    Suttiprapa S, Rinaldi G, Tsai IJ, Mann VH, Dubrovsky L et al.

    PLoS pathogens 2016;12;10;e1005931

  • Viability of developmental stages of Schistosoma mansoni quantified with xCELLigence worm real-time motility assay (xWORM).

    Rinaldi G, Loukas A, Brindley PJ, Irelan JT and Smout MJ

    International journal for parasitology. Drugs and drug resistance 2015;5;3;141-8

  • Carcinogenic Parasite Secretes Growth Factor That Accelerates Wound Healing and Potentially Promotes Neoplasia.

    Smout MJ, Sotillo J, Laha T, Papatpremsiri A, Rinaldi G et al.

    PLoS pathogens 2015;11;10;e1005209

  • RNA interference in Fasciola hepatica newly excysted juveniles: long dsRNA induces more persistent silencing than siRNA.

    Dell'Oca N, Basika T, Corvo I, Castillo E, Brindley PJ et al.

    Molecular and biochemical parasitology 2014;197;1-2;28-35

  • Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.

    Rinaldi G, Eckert SE, Tsai IJ, Suttiprapa S, Kines KJ et al.

    PLoS pathogens 2012;8;7;e1002820

  • Genetic manipulation of Schistosoma haematobium, the neglected schistosome.

    Rinaldi G, Okatcha TI, Popratiloff A, Ayuk MA, Suttiprapa S et al.

    PLoS neglected tropical diseases 2011;5;10;e1348

  • Development of functional genomic tools in trematodes: RNA interference and luciferase reporter gene activity in Fasciola hepatica.

    Rinaldi G, Morales ME, Cancela M, Castillo E, Brindley PJ and Tort JF

    PLoS neglected tropical diseases 2008;2;7;e260

  • Schistosoma mansoni infection is associated with quantitative and qualitative modifications of the mammalian intestinal microbiota.

    Jenkins TP, Peachey LE, Ajami NJ, MacDonald AS, Hsieh MH et al.

    Scientific reports 2018;8;1;12072

  • Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.

    Yan HB, Smout MJ, Ju C, Folley AE, Skinner DE et al.

    Antimicrobial agents and chemotherapy 2018;62;8

  • Expression, purification and characterization of two leucine aminopeptidases of the blood fluke, Schistosoma mansoni.

    Maggioli G, Rinaldi G, Giaudrone I, Berasain P, Tort JF et al.

    Molecular and biochemical parasitology 2018;219;17-23

  • Conservation and diversification of small RNA pathways within flatworms.

    Fontenla S, Rinaldi G, Smircich P and Tort JF

    BMC evolutionary biology 2017;17;1;215

  • Infection with Opisthorchis felineus induces intraepithelial neoplasia of the biliary tract in a rodent model.

    Gouveia MJ, Pakharukova MY, Laha T, Sripa B, Maksimova GA et al.

    Carcinogenesis 2017;38;9;929-937

  • MiR-277/4989 regulate transcriptional landscape during juvenile to adult transition in the parasitic helminth Schistosoma mansoni.

    Protasio AV, van Dongen S, Collins J, Quintais L, Ribeiro DM et al.

    PLoS neglected tropical diseases 2017;11;5;e0005559

  • Praziquantel for Schistosomiasis: Single-Drug Metabolism Revisited, Mode of Action, and Resistance.

    Vale N, Gouveia MJ, Rinaldi G, Brindley PJ, Gärtner F and Correia da Costa JM

    Antimicrobial agents and chemotherapy 2017;61;5

  • The role of estradiol metabolism in urogenital schistosomiasis-induced bladder cancer.

    Vale N, Gouveia MJ, Rinaldi G, Santos J, Santos LL et al.

    Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017;39;3;1010428317692247

  • Elevated prevalence of Helicobacter species and virulence factors in opisthorchiasis and associated hepatobiliary disease.

    Deenonpoe R, Mairiang E, Mairiang P, Pairojkul C, Chamgramol Y et al.

    Scientific reports 2017;7;42744

  • Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers.

    McNulty SN, Tort JF, Rinaldi G, Fischer K, Rosa BA et al.

    PLoS genetics 2017;13;1;e1006537

  • HIV-1 Integrates Widely throughout the Genome of the Human Blood Fluke Schistosoma mansoni.

    Suttiprapa S, Rinaldi G, Tsai IJ, Mann VH, Dubrovsky L et al.

    PLoS pathogens 2016;12;10;e1005931

  • Computational deconvolution of gene expression by individual host cellular subsets from microarray analyses of complex, parasite-infected whole tissues.

    Banskota N, Odegaard JI, Rinaldi G and Hsieh MH

    International journal for parasitology 2016;46;7;447-52

  • Viability of developmental stages of Schistosoma mansoni quantified with xCELLigence worm real-time motility assay (xWORM).

    Rinaldi G, Loukas A, Brindley PJ, Irelan JT and Smout MJ

    International journal for parasitology. Drugs and drug resistance 2015;5;3;141-8

  • Carcinogenic Parasite Secretes Growth Factor That Accelerates Wound Healing and Potentially Promotes Neoplasia.

    Smout MJ, Sotillo J, Laha T, Papatpremsiri A, Rinaldi G et al.

    PLoS pathogens 2015;11;10;e1005209

  • Apoptosis of cholangiocytes modulated by thioredoxin of carcinogenic liver fluke.

    Matchimakul P, Rinaldi G, Suttiprapa S, Mann VH, Popratiloff A et al.

    The international journal of biochemistry & cell biology 2015;65;72-80

  • Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni.

    Rinaldi G, Yan H, Nacif-Pimenta R, Matchimakul P, Bridger J et al.

    International journal for parasitology 2015;45;8;527-35

  • The role of estrogens and estrogen receptor signaling pathways in cancer and infertility: the case of schistosomes.

    Botelho MC, Alves H, Barros A, Rinaldi G, Brindley PJ and Sousa M

    Trends in parasitology 2015;31;6;246-50

  • Susceptibility of Snails to Infection with Schistosomes is influenced by Temperature and Expression of Heat Shock Proteins.

    Knight M, Elhelu O, Smith M, Haugen B, Miller A et al.

    Epidemiology (Sunnyvale, Calif.) 2015;5;2

  • A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma.

    Plieskatt J, Rinaldi G, Feng Y, Peng J, Easley S et al.

    BMC cancer 2015;15;309

  • Estrogen-like metabolites and DNA-adducts in urogenital schistosomiasis-associated bladder cancer.

    Gouveia MJ, Santos J, Brindley PJ, Rinaldi G, Lopes C et al.

    Cancer letters 2015;359;2;226-32

  • New research tools for urogenital schistosomiasis.

    Rinaldi G, Young ND, Honeycutt JD, Brindley PJ, Gasser RB and Hsieh MH

    The Journal of infectious diseases 2015;211;6;861-9

  • The miRNAome of Opisthorchis viverrini induced intrahepatic cholangiocarcinoma.

    Peng J, Feng Y, Rinaldi G, Yonglitthipagon P, Easley SE et al.

    Genomics data 2014;2;274-9

  • Profiling miRNAs in nasopharyngeal carcinoma FFPE tissue by microarray and Next Generation Sequencing.

    Peng J, Feng Y, Rinaldi G, Levine P, Easley S et al.

    Genomics data 2014;2;285-9

  • Distinct miRNA signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke Opisthorchis viverrini.

    Plieskatt JL, Rinaldi G, Feng Y, Peng J, Yonglitthipagon P et al.

    Journal of hepatology 2014;61;4;850-8

  • RNA interference in Fasciola hepatica newly excysted juveniles: long dsRNA induces more persistent silencing than siRNA.

    Dell'Oca N, Basika T, Corvo I, Castillo E, Brindley PJ et al.

    Molecular and biochemical parasitology 2014;197;1-2;28-35

  • Bioclojure: a functional library for the manipulation of biological sequences.

    Plieskatt J, Rinaldi G, Brindley PJ, Jia X, Potriquet J et al.

    Bioinformatics (Oxford, England) 2014;30;17;2537-9

  • Reversible paralysis of Schistosoma mansoni by forchlorfenuron, a phenylurea cytokinin that affects septins.

    Zeraik AE, Galkin VE, Rinaldi G, Garratt RC, Smout MJ et al.

    International journal for parasitology 2014;44;8;523-31

  • Pseudotyped murine leukemia virus for schistosome transgenesis: approaches, methods and perspectives.

    Mann VH, Suttiprapa S, Skinner DE, Brindley PJ and Rinaldi G

    Transgenic research 2014;23;3;539-56

  • How might flukes and tapeworms maintain genome integrity without a canonical piRNA pathway?

    Skinner DE, Rinaldi G, Koziol U, Brehm K and Brindley PJ

    Trends in parasitology 2014;30;3;123-9

  • Methods and matrices: approaches to identifying miRNAs for nasopharyngeal carcinoma.

    Plieskatt JL, Rinaldi G, Feng Y, Levine PH, Easley S et al.

    Journal of translational medicine 2014;12;3

  • Circumventing qPCR inhibition to amplify miRNAs in plasma.

    Plieskatt JL, Feng Y, Rinaldi G, Mulvenna JP, Bethony JM and Brindley PJ

    Biomarker research 2014;2;13

  • Schistosome and liver fluke derived catechol-estrogens and helminth associated cancers.

    Correia da Costa JM, Vale N, Gouveia MJ, Botelho MC, Sripa B et al.

    Frontiers in genetics 2014;5;444

  • Urinary estrogen metabolites and self-reported infertility in women infected with Schistosoma haematobium.

    Santos J, Gouveia MJ, Vale N, Delgado Mde L, Gonçalves A et al.

    PloS one 2014;9;5;e96774

  • Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract.

    Vale N, Gouveia MJ, Botelho M, Sripa B, Suttiprapa S et al.

    Parasitology international 2013;62;6;535-42

  • Septins of Platyhelminths: identification, phylogeny, expression and localization among developmental stages of Schistosoma mansoni.

    Zeraik AE, Rinaldi G, Mann VH, Popratiloff A, Araujo AP et al.

    PLoS neglected tropical diseases 2013;7;12;e2602

  • Tumour-like phenotypes in urothelial cells after exposure to antigens from eggs of Schistosoma haematobium: an oestrogen-DNA adducts mediated pathway?

    Botelho MC, Vale N, Gouveia MJ, Rinaldi G, Santos J et al.

    International journal for parasitology 2013;43;1;17-26

  • Human U6 promoter drives stronger shRNA activity than its schistosome orthologue in Schistosoma mansoni and human fibrosarcoma cells.

    Duvoisin R, Ayuk MA, Rinaldi G, Suttiprapa S, Mann VH et al.

    Transgenic research 2012;21;3;511-21

  • Prototypic chromatin insulator cHS4 protects retroviral transgene from silencing in Schistosoma mansoni.

    Suttiprapa S, Rinaldi G and Brindley PJ

    Transgenic research 2012;21;3;555-66

  • Genetic manipulation of schistosomes--progress with integration competent vectors.

    Suttiprapa S, Rinaldi G and Brindley PJ

    Parasitology 2012;139;5;641-50

  • Whole-genome sequence of Schistosoma haematobium.

    Young ND, Jex AR, Li B, Liu S, Yang L et al.

    Nature genetics 2012;44;2;221-5

  • An antibiotic selection marker for schistosome transgenesis.

    Rinaldi G, Suttiprapa S, Tort JF, Folley AE, Skinner DE and Brindley PJ

    International journal for parasitology 2012;42;1;123-30

  • Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.

    Rinaldi G, Eckert SE, Tsai IJ, Suttiprapa S, Kines KJ et al.

    PLoS pathogens 2012;8;7;e1002820

  • Vasa-Like DEAD-Box RNA Helicases of Schistosoma mansoni.

    Skinner DE, Rinaldi G, Suttiprapa S, Mann VH, Smircich P et al.

    PLoS neglected tropical diseases 2012;6;6;e1686

  • Genetic manipulation of Schistosoma haematobium, the neglected schistosome.

    Rinaldi G, Okatcha TI, Popratiloff A, Ayuk MA, Suttiprapa S et al.

    PLoS neglected tropical diseases 2011;5;10;e1348

  • Establishing transgenic schistosomes.

    Mann VH, Suttiprapa S, Rinaldi G and Brindley PJ

    PLoS neglected tropical diseases 2011;5;8;e1230

  • The metastasis-associated protein-1 gene encodes a host permissive factor for schistosomiasis, a leading global cause of inflammation and cancer.

    Nair SS, Bommana A, Bethony JM, Lyon AJ, Ohshiro K et al.

    Hepatology (Baltimore, Md.) 2011;54;1;285-95

  • Schistosoma mansoni U6 gene promoter-driven short hairpin RNA induces RNA interference in human fibrosarcoma cells and schistosomules.

    Ayuk MA, Suttiprapa S, Rinaldi G, Mann VH, Lee CM and Brindley PJ

    International journal for parasitology 2011;41;7;783-9

  • Quantitative retrotransposon anchored PCR confirms transduction efficiency of transgenes in adult Schistosoma mansoni.

    Rinaldi G, Suttiprapa S and Brindley PJ

    Molecular and biochemical parasitology 2011;177;1;70-6

  • Collagenolytic activities of the major secreted cathepsin L peptidases involved in the virulence of the helminth pathogen, Fasciola hepatica.

    Robinson MW, Corvo I, Jones PM, George AM, Padula MP et al.

    PLoS neglected tropical diseases 2011;5;4;e1012

  • Survey of transcripts expressed by the invasive juvenile stage of the liver fluke Fasciola hepatica.

    Cancela M, Ruétalo N, Dell'Oca N, da Silva E, Smircich P et al.

    BMC genomics 2010;11;227

  • Culture for genetic manipulation of developmental stages of Schistosoma mansoni.

    Mann VH, Morales ME, Rinaldi G and Brindley PJ

    Parasitology 2010;137;3;451-62

  • Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggs.

    Kines KJ, Rinaldi G, Okatcha TI, Morales ME, Mann VH et al.

    PLoS neglected tropical diseases 2010;4;2;e593

  • RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs.

    Rinaldi G, Morales ME, Alrefaei YN, Cancela M, Castillo E et al.

    Molecular and biochemical parasitology 2009;167;2;118-26

  • A distinctive repertoire of cathepsins is expressed by juvenile invasive Fasciola hepatica.

    Cancela M, Acosta D, Rinaldi G, Silva E, Durán R et al.

    Biochimie 2008;90;10;1461-75

  • Development of functional genomic tools in trematodes: RNA interference and luciferase reporter gene activity in Fasciola hepatica.

    Rinaldi G, Morales ME, Cancela M, Castillo E, Brindley PJ and Tort JF

    PLoS neglected tropical diseases 2008;2;7;e260

  • RNA interference of Schistosoma mansoni cathepsin D, the apical enzyme of the hemoglobin proteolysis cascade.

    Morales ME, Rinaldi G, Gobert GN, Kines KJ, Tort JF and Brindley PJ

    Molecular and biochemical parasitology 2008;157;2;160-8

Rinaldi, Gabriel
Gabriel's Timeline
2015

Joined Matt Berriman Group, Parasite Genomics, as Senior Staff Scientist, at the Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK

2012

Research Assistant Professor. Department of Immunology, Microbiology and Tropical Medicine, School of Medicine and Health Sciences, The George Washington University. Washington DC, USA

2011

Graduated from School of Science with Doctor of Philosophy (PhD) degree in Molecular and Cell Biology, University of the Republic, Uruguay

2009

Began PosDoc with Dr. Paul Brindley, Department of Immunology, Microbiology and Tropical Medicine, School of Medicine and Health Sciences, The George Washington University, Washington DC, USA

2008

Graduated from School of Science with Master in Science (MSc) degree in Molecular and Cell Biology, University of the Republic, Uruguay

2004

Assistant Professor in Genetics, Department of Genetics, School of Medicine, University of the Republic, Uruguay

2001

Graduated from School of Medicine with Medical Doctor (MD) degree, University of the Republic, Uruguay