Dr Mercedes Pardo Calvo | Senior Staff Scientist

Pardo Calvo, Mercedes

Mercedes is interested in understanding protein function and regulation through the study of protein interactions. Her research focuses on the characterisation of protein complexes in different systems, from mouse embryonic stem cells to the malaria parasite, using biochemical techniques.

The majority of proteins do not work in isolation. Instead they organise in stable multiprotein assemblies or engage in dynamic interactions with other proteins in order to perform their roles. Understanding proteins interactions is therefore crucial for elucidating protein function and regulation.

I use affinity purification in combination with mass spectrometry to characterise multiprotein complexes and protein-protein interactions. In collaboration with the Skarnes and Bradley labs I developed the endogenous tandem affinity purification technology in mouse embryonic stem cells. Much of my work using this strategy has focused on the study of chromatin-associated proteins important for embryonic stem cell biology and development. Another of my interests are enzymes that introduce post-translational modifications on proteins, especially those that are less well-known. More recently I have started using other complementary approaches to investigate protein complexes, mainly biochemical fractionation techniques such as blue native electrophoresis and size exclusion chromatography.

As part of my job I collaborate with several researchers here at the Sanger Institute and also in other research institutions in their quest to identify protein interactions. My role involves advising on experiment design, training on the experimental techniques, performing experiments, problem solving, and data analysis and biological interpretation.

I am also involved in training other scientists through the Wellcome Trust Advanced Courses. Here I teach a module comprising affinity purification and mass spectrometry in different contexts: Functional Genomics and Systems Biology, Protein Interactions and Networks, and Genome-wide Approaches with Fission Yeast (now discontinued).

Publications

  • Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity.

    Thomas DC, Clare S, Sowerby JM, Pardo M, Juss JK et al.

    The Journal of experimental medicine 2017;214;4;1111-1128

  • Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells.

    Bode D, Yu L, Tate P, Pardo M and Choudhary J

    Molecular & cellular proteomics : MCP 2016;15;3;878-91

  • p53 mediates loss of hematopoietic stem cell function and lymphopenia in Mysm1 deficiency.

    Belle JI, Langlais D, Petrov JC, Pardo M, Jones RG et al.

    Blood 2015;125;15;2344-8

  • Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex.

    Sedgwick GG, Hayward DG, Di Fiore B, Pardo M, Yu L et al.

    The EMBO journal 2013;32;2;303-14

  • Nuclear receptor binding protein 1 regulates intestinal progenitor cell homeostasis and tumour formation.

    Wilson CH, Crombie C, van der Weyden L, Poulogiannis G, Rust AG et al.

    The EMBO journal 2012;31;11;2486-97

  • Assignment of protein interactions from affinity purification/mass spectrometry data.

    Pardo M and Choudhary JS

    Journal of proteome research 2012;11;3;1462-74

  • Prmt5 is essential for early mouse development and acts in the cytoplasm to maintain ES cell pluripotency.

    Tee WW, Pardo M, Theunissen TW, Yu L, Choudhary JS et al.

    Genes & development 2010;24;24;2772-7

  • An expanded Oct4 interaction network: implications for stem cell biology, development, and disease.

    Pardo M, Lang B, Yu L, Prosser H, Bradley A et al.

    Cell stem cell 2010;6;4;382-95

  • The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast.

    Pardo M and Nurse P

    Journal of cell science 2005;118;Pt 8;1705-14

  • Equatorial retention of the contractile actin ring by microtubules during cytokinesis.

    Pardo M and Nurse P

    Science (New York, N.Y.) 2003;300;5625;1569-74

  • Multiple short windows of Calcium-Dependent Protein Kinase 4 activity coordinate distinct cell cycle events during Plasmodium gametogenesis.

    Fang H, Klages N, Baechler B, Hillner E, Yu L et al.

    eLife 2017;6

  • Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity.

    Thomas DC, Clare S, Sowerby JM, Pardo M, Juss JK et al.

    The Journal of experimental medicine 2017;214;4;1111-1128

  • Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling.

    Pardo M, Bode D, Yu L and Choudhary JS

    Journal of visualized experiments : JoVE 2017;122

  • Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells.

    Bode D, Yu L, Tate P, Pardo M and Choudhary J

    Molecular & cellular proteomics : MCP 2016;15;3;878-91

  • p53 mediates loss of hematopoietic stem cell function and lymphopenia in Mysm1 deficiency.

    Belle JI, Langlais D, Petrov JC, Pardo M, Jones RG et al.

    Blood 2015;125;15;2344-8

  • Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex.

    Sedgwick GG, Hayward DG, Di Fiore B, Pardo M, Yu L et al.

    The EMBO journal 2013;32;2;303-14

  • Nuclear receptor binding protein 1 regulates intestinal progenitor cell homeostasis and tumour formation.

    Wilson CH, Crombie C, van der Weyden L, Poulogiannis G, Rust AG et al.

    The EMBO journal 2012;31;11;2486-97

  • Assignment of protein interactions from affinity purification/mass spectrometry data.

    Pardo M and Choudhary JS

    Journal of proteome research 2012;11;3;1462-74

  • Prmt5 is essential for early mouse development and acts in the cytoplasm to maintain ES cell pluripotency.

    Tee WW, Pardo M, Theunissen TW, Yu L, Choudhary JS et al.

    Genes & development 2010;24;24;2772-7

  • An expanded Oct4 interaction network: implications for stem cell biology, development, and disease.

    Pardo M, Lang B, Yu L, Prosser H, Bradley A et al.

    Cell stem cell 2010;6;4;382-95

  • Molecular characterization of the Salmonella enterica serovar Typhi Vi-typing bacteriophage E1.

    Pickard D, Thomson NR, Baker S, Wain J, Pardo M et al.

    Journal of bacteriology 2008;190;7;2580-7

  • Genetic and proteomic evidences support the localization of yeast enolase in the cell surface.

    López-Villar E, Monteoliva L, Larsen MR, Sachon E, Shabaz M et al.

    Proteomics 2006;6 Suppl 1;S107-18

  • The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast.

    Pardo M and Nurse P

    Journal of cell science 2005;118;Pt 8;1705-14

  • PST1 and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity.

    Pardo M, Monteoliva L, Vázquez P, Martínez R, Molero G et al.

    Microbiology (Reading, England) 2004;150;Pt 12;4157-70

  • Equatorial retention of the contractile actin ring by microtubules during cytokinesis.

    Pardo M and Nurse P

    Science (New York, N.Y.) 2003;300;5625;1569-74

Pardo Calvo, Mercedes
Mercedes's Timeline
2011

Senior Staff Scientist, Proteomic Mass spectrometry, WTSI

2008

Staff Scientist, Proteomic Mass Spectrometry, WTSI

2004

Joined the Proteomic Mass Spectrometry team at WTSI as Research Associate

2000

Post-doctoral fellow in the Cell Cycle Lab at Cancer Research UK London Research Institute, under the supervision of Sir Paul Nurse

PhD in Microbiology from Universidad Complutense de Madrid, Spain

1998

Spent 4 months as visiting student in the Bussey Lab at the Department of Biology, McGill University, Canada

1995

Joined the Department of Microbiology at the School of Pharmacy (UCM, Spain) as PhD candidate, under the supervision of Drs Nombela and Gil

BSc in Pharmacy from Universidad Complutense de Madrid, Spain