Bradley, Allan

Allan Bradley and his research team moved to the University of Cambridge in 2019. This page is being maintained as a historical record of his research at the Sanger Institute and is no longer being updated

allan.bradley@sanger.ac.uk

Archive Page: Bradley Group

Allan served as the Institute’s Director from 2000 to 2010. During his ten-year tenure he shifted the Institute’s scientific programmes from a sequence production and informatics to a more genetically and biologically focused academic genome centre. He emphasised investment in young scientists, recruiting a cadre of young PIs, established international PhD programs for both basic and clinically trained candidates.

Professor Bradley led a research group through his tenure as Institute Director and continues to do so as a Senior Group Leader. Allan’s laboratory environment emphasises intellectual rigour but with a flair for innovation and creativity. His laboratory’s research is strongly focused on using genetics to discover genes involved in various biological systems, using somatic mutagenesis in mice to discover cancer genes and genetic screens in ES cells in culture. He is considers himself to be an “educator”, inspiring and mentoring graduate students and fellows. His laboratory is an active and successful training environment for postdoctoral and clinical fellows. Professor Bradley places special emphasis in mentoring and preparing young scientists for independent academic positions both in the UK and globally. Laboratory alumni include 22 independent PIs in 6 countries of whom 10 are full professors, or equivalent.

Allan is also a serial entrepreneur, establishing several biotechnology companies. While in the USA he founded two companies; Lexicon Pharmaceuticals Inc. is publicly traded while Spectral Genomics Inc. was acquired. In 2010 he founded Kymab Ltd., which now employs more than 80 scientists. Many of Prof. Bradley’s trainees have joined these spin-outs.

Publications

Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines.

Bradley A, Evans M, Kaufman MH and Robertson E

Nature 1984;309;5965;255-6

PUBMED: 6717601; DOI: 10.1038/309255a0
A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice.

Kuehn MR, Bradley A, Robertson EJ and Evans MJ

Nature 1987;326;6110;295-8

PUBMED: 3029599; DOI: 10.1038/326295a0
Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours.

Donehower LA, Harvey M, Slagle BL, McArthur MJ, Montgomery CA et al.

Nature 1992;356;6366;215-21

PUBMED: 1552940; DOI: 10.1038/356215a0
Mice deficient for Rb are nonviable and show defects in neurogenesis and haematopoiesis.

Lee EY, Chang CY, Hu N, Wang YC, Lai CC et al.

Nature 1992;359;6393;288-94

PUBMED: 1406932; DOI: 10.1038/359288a0
Alpha-inhibin is a tumour-suppressor gene with gonadal specificity in mice.

Matzuk MM, Finegold MJ, Su JG, Hsueh AJ and Bradley A

Nature 1992;360;6402;313-9

PUBMED: 1448148; DOI: 10.1038/360313a0
Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2.

Sharan SK, Morimatsu M, Albrecht U, Lim DS, Regel E et al.

Nature 1997;386;6627;804-10

PUBMED: 9126738; DOI: 10.1038/386804a0
p63 is a p53 homologue required for limb and epidermal morphogenesis.

Mills AA, Zheng B, Wang XJ, Vogel H, Roop DR and Bradley A

Nature 1999;398;6729;708-13

PUBMED: 10227293; DOI: 10.1038/19531
Engineering a mouse balancer chromosome.

Zheng B, Sage M, Cai WW, Thompson DM, Tavsanli BC et al.

Nature genetics 1999;22;4;375-8

PUBMED: 10431243; DOI: 10.1038/11949
Cancer predisposition caused by elevated mitotic recombination in Bloom mice.

Luo G, Santoro IM, McDaniel LD, Nishijima I, Mills M et al.

Nature genetics 2000;26;4;424-9

PUBMED: 11101838; DOI: 10.1038/82548
Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells.

Guo G, Wang W and Bradley A

Nature 2004;429;6994;891-5

PUBMED: 15215866; DOI: 10.1038/nature02653
Requirement of bic/microRNA-155 for normal immune function.

Rodriguez A, Vigorito E, Clare S, Warren MV, Couttet P et al.

Science (New York, N.Y.) 2007;316;5824;608-11

PUBMED: 17463290; PMC: 2610435; DOI: 10.1126/science.1139253
Abnormal behavior in a chromosome-engineered mouse model for human 15q11-13 duplication seen in autism.

Nakatani J, Tamada K, Hatanaka F, Ise S, Ohta H et al.

Cell 2009;137;7;1235-46

PUBMED: 19563756; PMC: 3710970; DOI: 10.1016/j.cell.2009.04.024
A conditional knockout resource for the genome-wide study of mouse gene function.

Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W et al.

Nature 2011;474;7351;337-42

PUBMED: 21677750; PMC: 3572410; DOI: 10.1038/nature10163
A resource of vectors and ES cells for targeted deletion of microRNAs in mice.

Prosser HM, Koike-Yusa H, Cooper JD, Law FC and Bradley A

Nature biotechnology 2011;29;9;840-5

PUBMED: 21822254; PMC: 3242032; DOI: 10.1038/nbt.1929
Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.

White JK, Gerdin AK, Karp NA, Ryder E, Buljan M et al.

Cell 2013;154;2;452-64

PUBMED: 23870131; PMC: 3717207; DOI: 10.1016/j.cell.2013.06.022
Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery.

Lee EC, Liang Q, Ali H, Bayliss L, Beasley A et al.

Nature biotechnology 2014;32;4;356-63

PUBMED: 24633243; DOI: 10.1038/nbt.2825

FAMIN Is a Multifunctional Purine Enzyme Enabling the Purine Nucleotide Cycle.

Cader MZ, de Almeida Rodrigues RP, West JA, Sewell GW, Md-Ibrahim MN et al.

Cell 2020;180;2;278-295.e23

PUBMED: 31978345; DOI: 10.1016/j.cell.2019.12.017
High-throughput discovery of genetic determinants of circadian misalignment.

Zhang T, Xie P, Dong Y, Liu Z, Zhou F et al.

PLoS genetics 2020;16;1;e1008577

PUBMED: 31929527; DOI: 10.1371/journal.pgen.1008577

A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Salmonella Infection.

Yeung ATY, Choi YH, Lee AHY, Hale C, Ponstingl H et al.

mBio 2019;10;5

PUBMED: 31594818; DOI: 10.1128/mBio.02169-19
NPM1c alters FLT3-D835Y localization and signaling in acute myeloid leukemia.

Rudorf A, Müller TA, Klingeberg C, Kreutmair S, Poggio T et al.

Blood 2019;134;4;383-388

PUBMED: 31186273; PMC: 6659255; DOI: 10.1182/blood.2018883140
Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming.

Yang J, Rajan SS, Friedrich MJ, Lan G, Zou X et al.

Stem cell reports 2019;12;4;757-771

PUBMED: 30905739; PMC: 6450436; DOI: 10.1016/j.stemcr.2019.02.010
PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice.

Weber J, de la Rosa J, Grove CS, Schick M, Rad L et al.

Nature communications 2019;10;1;1415

PUBMED: 30926791; PMC: 6440946; DOI: 10.1038/s41467-019-09180-3
ATM orchestrates the DNA-damage response to counter toxic non-homologous end-joining at broken replication forks.

Balmus G, Pilger D, Coates J, Demir M, Sczaniecka-Clift M et al.

Nature communications 2019;10;1;87

PUBMED: 30622252; PMC: 6325118; DOI: 10.1038/s41467-018-07729-2
PiggyBac Transposon-Based Insertional Mutagenesis in Mice.

Friedrich MJ, Bronner IF, Liu P, Bradley A and Rad R

Methods in molecular biology (Clifton, N.J.) 2019;1907;171-183

PUBMED: 30543000; DOI: 10.1007/978-1-4939-8967-6_14

SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4.

Tzelepis K, De Braekeleer E, Aspris D, Barbieri I, Vijayabaskar MS et al.

Nature communications 2018;9;1;5378

PUBMED: 30568163; PMC: 6300607; DOI: 10.1038/s41467-018-07620-0
Repair of double-strand breaks induced by CRISPR-Cas9 leads to large deletions and complex rearrangements.

Kosicki M, Tomberg K and Bradley A

Nature biotechnology 2018;36;8;765-771

PUBMED: 30010673; PMC: 6390938; DOI: 10.1038/nbt.4192
Inter-homologue repair in fertilized human eggs?

Egli D, Zuccaro MV, Kosicki M, Church GM, Bradley A and Jasin M

Nature 2018;560;7717;E5-E7

PUBMED: 30089924; DOI: 10.1038/s41586-018-0379-5
Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci.

Martin J, Ponstingl H, Lefranc MP, Archer J, Sargan D and Bradley A

Immunogenetics 2018;70;4;223-236

PUBMED: 28924718; PMC: 5871656; DOI: 10.1007/s00251-017-1028-0
Evolutionary routes and KRAS dosage define pancreatic cancer phenotypes.

Mueller S, Engleitner T, Maresch R, Zukowska M, Lange S et al.

Nature 2018;554;7690;62-68

PUBMED: 29364867; PMC: 6097607; DOI: 10.1038/nature25459

Revealing hidden complexities of genomic rearrangements generated with Cas9.

Boroviak K, Fu B, Yang F, Doe B and Bradley A

Scientific reports 2017;7;1;12867

PUBMED: 28993641; PMC: 5634419; DOI: 10.1038/s41598-017-12740-6
Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.

Meehan TF, Conte N, West DB, Jacobsen JO, Mason J et al.

Nature genetics 2017;49;8;1231-1238

PUBMED: 28650483; PMC: 5546242; DOI: 10.1038/ng.3901
Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.

Metzakopian E, Strong A, Iyer V, Hodgkins A, Tzelepis K et al.

Scientific reports 2017;7;1;2244

PUBMED: 28533524; PMC: 5440395; DOI: 10.1038/s41598-017-01766-5
A single-copy Sleeping Beauty transposon mutagenesis screen identifies new PTEN-cooperating tumor suppressor genes.

de la Rosa J, Weber J, Friedrich MJ, Li Y, Rad L et al.

Nature genetics 2017;49;5;730-741

PUBMED: 28319090; PMC: 5409503; DOI: 10.1038/ng.3817
Deletion of the MAD2L1 spindle assembly checkpoint gene is tolerated in mouse models of acute T-cell lymphoma and hepatocellular carcinoma.

Foijer F, Albacker LA, Bakker B, Spierings DC, Yue Y et al.

eLife 2017;6

PUBMED: 28318489; DOI: 10.7554/eLife.20873
Genome-wide transposon screening and quantitative insertion site sequencing for cancer gene discovery in mice.

Friedrich MJ, Rad L, Bronner IF, Strong A, Wang W et al.

Nature protocols 2017;12;2;289-309

PUBMED: 28079877; DOI: 10.1038/nprot.2016.164
Disentangling PTEN-cooperating tumor suppressor gene networks in cancer.

de la Rosa J, Weber J, Rad R, Bradley A and Cadiñanos J

Molecular & cellular oncology 2017;4;4;e1325550

PUBMED: 28868343; PMC: 5540202; DOI: 10.1080/23723556.2017.1325550

High-throughput discovery of novel developmental phenotypes.

Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD et al.

Nature 2016;537;7621;508-514

PUBMED: 27626380; PMC: 5295821; DOI: 10.1038/nature19356
Priming HIV-1 broadly neutralizing antibody precursors in human Ig loci transgenic mice.

Sok D, Briney B, Jardine JG, Kulp DW, Menis S et al.

Science (New York, N.Y.) 2016

PUBMED: 27608668; DOI: 10.1126/science.aah3945
C13orf31 (FAMIN) is a central regulator of immunometabolic function.

Cader MZ, Boroviak K, Zhang Q, Assadi G, Kempster SL et al.

Nature immunology 2016;17;9;1046-56

PUBMED: 27478939; PMC: 6581540; DOI: 10.1038/ni.3532
Aneuploid embryonic stem cells exhibit impaired differentiation and increased neoplastic potential.

Zhang M, Cheng L, Jia Y, Liu G, Li C et al.

The EMBO journal 2016

PUBMED: 27558554; DOI: 10.15252/embj.201593103
Single-cell sequencing reveals karyotype heterogeneity in murine and human malignancies.

Bakker B, Taudt A, Belderbos ME, Porubsky D, Spierings DC et al.

Genome biology 2016;17;1;115

PUBMED: 27246460; PMC: 4888588; DOI: 10.1186/s13059-016-0971-7
Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.

Rouhani FJ, Nik-Zainal S, Wuster A, Li Y, Conte N et al.

PLoS genetics 2016;12;4;e1005932

PUBMED: 27054363; PMC: 4824386; DOI: 10.1371/journal.pgen.1005932
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice.

Maresch R, Mueller S, Veltkamp C, Öllinger R, Friedrich M et al.

Nature communications 2016;7;10770

PUBMED: 26916719; PMC: 4773438; DOI: 10.1038/ncomms10770
Chromosome engineering in zygotes with CRISPR/Cas9.

Boroviak K, Doe B, Banerjee R, Yang F and Bradley A

Genesis (New York, N.Y. : 2000) 2016;54;2;78-85

PUBMED: 26742453; PMC: 4819711; DOI: 10.1002/dvg.22915
Genome wide conditional mouse knockout resources

Kaloff, C, Anastassiadis, K, Ayadi et al.

Drug Discovery Today: Disease Models 2016;20;3;12

DOI: 10.1016/j.ddmod.2017.08.002

CRISPR/Cas9 somatic multiplex-mutagenesis for high-throughput functional cancer genomics in mice.

Weber J, Öllinger R, Friedrich M, Ehmer U, Barenboim M et al.

Proceedings of the National Academy of Sciences of the United States of America 2015;112;45;13982-7

PUBMED: 26508638; PMC: 4653208; DOI: 10.1073/pnas.1512392112
High-density P300 enhancers control cell state transitions.

Witte S, Bradley A, Enright AJ and Muljo SA

BMC genomics 2015;16;903

PUBMED: 26546038; PMC: 4636788; DOI: 10.1186/s12864-015-1905-6
A Synergistic Interaction between Chk1- and MK2 Inhibitors in KRAS-Mutant Cancer.

Dietlein F, Kalb B, Jokic M, Noll EM, Strong A et al.

Cell 2015;162;1;146-59

PUBMED: 26140595; DOI: 10.1016/j.cell.2015.05.053
Off-target assessment of CRISPR-Cas9 guiding RNAs in human iPS and mouse ES cells.

Tan EP, Li Y, Velasco-Herrera Mdel C, Yusa K and Bradley A

Genesis (New York, N.Y. : 2000) 2015;53;2;225-36

PUBMED: 25378133; DOI: 10.1002/dvg.22835
A conditional piggyBac transposition system for genetic screening in mice identifies oncogenic networks in pancreatic cancer.

Rad R, Rad L, Wang W, Strong A, Ponstingl H et al.

Nature genetics 2015;47;1;47-56

PUBMED: 25485836; DOI: 10.1038/ng.3164

A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer.

Schönhuber N, Seidler B, Schuck K, Veltkamp C, Schachtler C et al.

Nature medicine 2014;20;11;1340-1347

PUBMED: 25326799; PMC: 4270133; DOI: 10.1038/nm.3646
Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.

DiTommaso T, Jones LK, Cottle DL, WTSI Mouse Genetics Program, Gerdin AK et al.

PLoS genetics 2014;10;10;e1004705

PUBMED: 25340873; PMC: 4207618; DOI: 10.1371/journal.pgen.1004705
Chromosome instability induced by Mps1 and p53 mutation generates aggressive lymphomas exhibiting aneuploidy-induced stress.

Foijer F, Xie SZ, Simon JE, Bakker PL, Conte N et al.

Proceedings of the National Academy of Sciences of the United States of America 2014;111;37;13427-32

PUBMED: 25197064; PMC: 4169945; DOI: 10.1073/pnas.1400892111
Genetic background drives transcriptional variation in human induced pluripotent stem cells.

Rouhani F, Kumasaka N, de Brito MC, Bradley A, Vallier L and Gaffney D

PLoS genetics 2014;10;6;e1004432

PUBMED: 24901476; PMC: 4046971; DOI: 10.1371/journal.pgen.1004432
Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery.

Lee EC, Liang Q, Ali H, Bayliss L, Beasley A et al.

Nature biotechnology 2014;32;4;356-63

PUBMED: 24633243; DOI: 10.1038/nbt.2825

MiR-210 is induced by Oct-2, regulates B cells, and inhibits autoantibody production.

Mok Y, Schwierzeck V, Thomas DC, Vigorito E, Rayner TF et al.

Journal of immunology (Baltimore, Md. : 1950) 2013;191;6;3037-3048

PUBMED: 23960236; PMC: 4162006; DOI: 10.4049/jimmunol.1301289
A powerful molecular synergy between mutant Nucleophosmin and Flt3-ITD drives acute myeloid leukemia in mice.

Mupo A, Celani L, Dovey O, Cooper JL, Grove C et al.

Leukemia 2013;27;9;1917-20

PUBMED: 23478666; PMC: 3768110; DOI: 10.1038/leu.2013.77
Detailed molecular characterisation of acute myeloid leukaemia with a normal karyotype using targeted DNA capture.

Conte N, Varela I, Grove C, Manes N, Yusa K et al.

Leukemia 2013;27;9;1820-5

PUBMED: 23702683; PMC: 3768109; DOI: 10.1038/leu.2013.117
Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.

White JK, Gerdin AK, Karp NA, Ryder E, Buljan M et al.

Cell 2013;154;2;452-64

PUBMED: 23870131; PMC: 3717207; DOI: 10.1016/j.cell.2013.06.022
A genetic progression model of Braf(V600E)-induced intestinal tumorigenesis reveals targets for therapeutic intervention.

Rad R, Cadiñanos J, Rad L, Varela I, Strong A et al.

Cancer cell 2013;24;1;15-29

PUBMED: 23845441; PMC: 3706745; DOI: 10.1016/j.ccr.2013.05.014
Histone deacetylase 1 and 2 are essential for normal T-cell development and genomic stability in mice.

Dovey OM, Foster CT, Conte N, Edwards SA, Edwards JM et al.

Blood 2013;121;8;1335-44

PUBMED: 23287868; PMC: 3836254; DOI: 10.1182/blood-2012-07-441949
Prelamin A causes progeria through cell-extrinsic mechanisms and prevents cancer invasion.

de la Rosa J, Freije JM, Cabanillas R, Osorio FG, Fraga MF et al.

Nature communications 2013;4;2268

PUBMED: 23917225; PMC: 3758871; DOI: 10.1038/ncomms3268

Mouse large-scale phenotyping initiatives: overview of the European Mouse Disease Clinic (EUMODIC) and of the Wellcome Trust Sanger Institute Mouse Genetics Project.

Ayadi A, Birling MC, Bottomley J, Bussell J, Fuchs H et al.

Mammalian genome : official journal of the International Mammalian Genome Society 2012;23;9-10;600-10

PUBMED: 22961258; PMC: 3463797; DOI: 10.1007/s00335-012-9418-y

A resource of vectors and ES cells for targeted deletion of microRNAs in mice.

Prosser HM, Koike-Yusa H, Cooper JD, Law FC and Bradley A

Nature biotechnology 2011;29;9;840-5

PUBMED: 21822254; PMC: 3242032; DOI: 10.1038/nbt.1929
A conditional knockout resource for the genome-wide study of mouse gene function.

Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W et al.

Nature 2011;474;7351;337-42

PUBMED: 21677750; PMC: 3572410; DOI: 10.1038/nature10163
Genetic screens using the piggyBac transposon.

Chew SK, Rad R, Futreal PA, Bradley A and Liu P

Methods (San Diego, Calif.) 2011;53;4;366-71

PUBMED: 21185377; PMC: 3650578; DOI: 10.1016/j.ymeth.2010.12.022
A hyperactive piggyBac transposase for mammalian applications.

Yusa K, Zhou L, Li MA, Bradley A and Craig NL

Proceedings of the National Academy of Sciences of the United States of America 2011;108;4;1531-6

PUBMED: 21205896; PMC: 3029773; DOI: 10.1073/pnas.1008322108

PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice.

Rad R, Rad L, Wang W, Cadinanos J, Vassiliou G et al.

Science (New York, N.Y.) 2010;330;6007;1104-7

PUBMED: 20947725; PMC: 3719098; DOI: 10.1126/science.1193004
Ectodomains of the LDL receptor-related proteins LRP1b and LRP4 have anchorage independent functions in vivo.

Dietrich MF, van der Weyden L, Prosser HM, Bradley A, Herz J and Adams DJ

PloS one 2010;5;4;e9960

PUBMED: 20383322; PMC: 2850915; DOI: 10.1371/journal.pone.0009960
An expanded Oct4 interaction network: implications for stem cell biology, development, and disease.

Pardo M, Lang B, Yu L, Prosser H, Bradley A et al.

Cell stem cell 2010;6;4;382-95

PUBMED: 20362542; PMC: 2860244; DOI: 10.1016/j.stem.2010.03.004
The use of DNA transposons for cancer gene discovery in mice.

Vassiliou G, Rad R and Bradley A

Methods in enzymology 2010;477;91-106

PUBMED: 20699138; DOI: 10.1016/S0076-6879(10)77006-3

Discovery of candidate disease genes in ENU-induced mouse mutants by large-scale sequencing, including a splice-site mutation in nucleoredoxin.

Boles MK, Wilkinson BM, Wilming LG, Liu B, Probst FJ et al.

PLoS genetics 2009;5;12;e1000759

PUBMED: 20011118; PMC: 2782131; DOI: 10.1371/journal.pgen.1000759
Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM et al.

Nature methods 2009;6;7;493-5

PUBMED: 19525957; PMC: 3555078; DOI: 10.1038/nmeth.1342
Abnormal behavior in a chromosome-engineered mouse model for human 15q11-13 duplication seen in autism.

Nakatani J, Tamada K, Hatanaka F, Ise S, Ohta H et al.

Cell 2009;137;7;1235-46

PUBMED: 19563756; PMC: 3710970; DOI: 10.1016/j.cell.2009.04.024
Chromosomal mobilization and reintegration of Sleeping Beauty and PiggyBac transposons.

Liang Q, Kong J, Stalker J and Bradley A

Genesis (New York, N.Y. : 2000) 2009;47;6;404-8

PUBMED: 19391106; DOI: 10.1002/dvg.20508
Transposon-mediated genome manipulation in vertebrates.

Ivics Z, Li MA, Mátés L, Boeke JD, Nagy A et al.

Nature methods 2009;6;6;415-22

PUBMED: 19478801; PMC: 2867038; DOI: 10.1038/nmeth.1332
Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon.

Yusa K, Rad R, Takeda J and Bradley A

Nature methods 2009;6;5;363-9

PUBMED: 19337237; PMC: 2677165; DOI: 10.1038/nmeth.1323
A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells.

Wang W, Bradley A and Huang Y

Genome research 2009;19;4;667-73

PUBMED: 19233961; PMC: 2665785; DOI: 10.1101/gr.085621.108

A DNA transposon-based approach to validate oncogenic mutations in the mouse.

Su Q, Prosser HM, Campos LS, Ortiz M, Nakamura T et al.

Proceedings of the National Academy of Sciences of the United States of America 2008;105;50;19904-9

PUBMED: 19064922; PMC: 2597691; DOI: 10.1073/pnas.0807785105
Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster.

Lange UC, Adams DJ, Lee C, Barton S, Schneider R et al.

Molecular and cellular biology 2008;28;15;4688-96

PUBMED: 18505827; PMC: 2493357; DOI: 10.1128/MCB.00272-08
Loss of Rassf1a cooperates with Apc(Min) to accelerate intestinal tumourigenesis.

van der Weyden L, Arends MJ, Dovey OM, Harrison HL, Lefebvre G et al.

Oncogene 2008;27;32;4503-8

PUBMED: 18391979; PMC: 3706934; DOI: 10.1038/onc.2008.94
Chromosomal transposition of PiggyBac in mouse embryonic stem cells.

Wang W, Lin C, Lu D, Ning Z, Cox T et al.

Proceedings of the National Academy of Sciences of the United States of America 2008;105;27;9290-5

PUBMED: 18579772; PMC: 2440425; DOI: 10.1073/pnas.0801017105
Mosaic complementation demonstrates a regulatory role for myosin VIIa in actin dynamics of stereocilia.

Prosser HM, Rzadzinska AK, Steel KP and Bradley A

Molecular and cellular biology 2008;28;5;1702-12

PUBMED: 18160714; PMC: 2258769; DOI: 10.1128/MCB.01282-07

microRNA-155 regulates the generation of immunoglobulin class-switched plasma cells.

Vigorito E, Perks KL, Abreu-Goodger C, Bunting S, Xiang Z et al.

Immunity 2007;27;6;847-59

PUBMED: 18055230; PMC: 4135426; DOI: 10.1016/j.immuni.2007.10.009
Requirement of bic/microRNA-155 for normal immune function.

Rodriguez A, Vigorito E, Clare S, Warren MV, Couttet P et al.

Science (New York, N.Y.) 2007;316;5824;608-11

PUBMED: 17463290; PMC: 2610435; DOI: 10.1126/science.1139253
A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells.

Wang W and Bradley A

Genome biology 2007;8;4;R48

PUBMED: 17407599; PMC: 1895998; DOI: 10.1186/gb-2007-8-4-r48
A Sall4 mutant mouse model useful for studying the role of Sall4 in early embryonic development and organogenesis.

Warren M, Wang W, Spiden S, Chen-Murchie D, Tannahill D et al.

Genesis (New York, N.Y. : 2000) 2007;45;1;51-8

PUBMED: 17216607; PMC: 2593393; DOI: 10.1002/dvg.20264
Generation of an inducible and optimized piggyBac transposon system.

Cadiñanos J and Bradley A

Nucleic acids research 2007;35;12;e87

PUBMED: 17576687; PMC: 1919496; DOI: 10.1093/nar/gkm446

Functional knockout of the matrilin-3 gene causes premature chondrocyte maturation to hypertrophy and increases bone mineral density and osteoarthritis.

van der Weyden L, Wei L, Luo J, Yang X, Birk DE et al.

The American journal of pathology 2006;169;2;515-27

PUBMED: 16877353; PMC: 1698783; DOI: 10.2353/ajpath.2006.050981
TranscriptSNPView: a genome-wide catalog of mouse coding variation.

Cunningham F, Rios D, Griffiths M, Smith J, Ning Z et al.

Nature genetics 2006;38;8;853

PUBMED: 16874317; PMC: 2610433; DOI: 10.1038/ng0806-853a
Geminin is essential to prevent endoreduplication and to form pluripotent cells during mammalian development.

Gonzalez MA, Tachibana KE, Adams DJ, van der Weyden L, Hemberger M et al.

Genes & development 2006;20;14;1880-4

PUBMED: 16847348; PMC: 1522086; DOI: 10.1101/gad.379706
A deficiency in the region homologous to human 17q21.33-q23.2 causes heart defects in mice.

Yu YE, Morishima M, Pao A, Wang DY, Wen XY et al.

Genetics 2006;173;1;297-307

PUBMED: 16489219; PMC: 1461454; DOI: 10.1534/genetics.105.054833
Loss of TSLC1 causes male infertility due to a defect at the spermatid stage of spermatogenesis.

van der Weyden L, Arends MJ, Chausiaux OE, Ellis PJ, Lange UC et al.

Molecular and cellular biology 2006;26;9;3595-609

PUBMED: 16611999; PMC: 1447413; DOI: 10.1128/MCB.26.9.3595-3609.2006
DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.

Zody MC, Garber M, Adams DJ, Sharpe T, Harrow J et al.

Nature 2006;440;7087;1045-9

PUBMED: 16625196; PMC: 2610434; DOI: 10.1038/nature04689

A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction.

Adams DJ, Quail MA, Cox T, van der Weyden L, Gorick BD et al.

Genomics 2005;86;6;753-8

PUBMED: 16257172; DOI: 10.1016/j.ygeno.2005.08.003
The molecular clock mediates leptin-regulated bone formation.

Fu L, Patel MS, Bradley A, Wagner EF and Karsenty G

Cell 2005;122;5;803-15

PUBMED: 16143109; DOI: 10.1016/j.cell.2005.06.028
The RASSF1A isoform of RASSF1 promotes microtubule stability and suppresses tumorigenesis.

van der Weyden L, Tachibana KK, Gonzalez MA, Adams DJ, Ng BL et al.

Molecular and cellular biology 2005;25;18;8356-67

PUBMED: 16135822; PMC: 1234312; DOI: 10.1128/MCB.25.18.8356-8367.2005
Null and conditional semaphorin 3B alleles using a flexible puroDeltatk loxP/FRT vector.

van der Weyden L, Adams DJ, Harris LW, Tannahill D, Arends MJ and Bradley A

Genesis (New York, N.Y. : 2000) 2005;41;4;171-8

PUBMED: 15789413; DOI: 10.1002/gene.20111
BRCTx is a novel, highly conserved RAD18-interacting protein.

Adams DJ, van der Weyden L, Gergely FV, Arends MJ, Ng BL et al.

Molecular and cellular biology 2005;25;2;779-88

PUBMED: 15632077; PMC: 543416; DOI: 10.1128/MCB.25.2.779-788.2005

Identification of mammalian microRNA host genes and transcription units.

Rodriguez A, Griffiths-Jones S, Ashurst JL and Bradley A

Genome research 2004;14;10A;1902-10

PUBMED: 15364901; PMC: 524413; DOI: 10.1101/gr.2722704
The knockout mouse project.

Austin CP, Battey JF, Bradley A, Bucan M, Capecchi M et al.

Nature genetics 2004;36;9;921-4

PUBMED: 15340423; PMC: 2716027; DOI: 10.1038/ng0904-921
The European dimension for the mouse genome mutagenesis program.

Auwerx J, Avner P, Baldock R, Ballabio A, Balling R et al.

Nature genetics 2004;36;9;925-7

PUBMED: 15340424; PMC: 2716028; DOI: 10.1038/ng0904-925
Mutagenic insertion and chromosome engineering resource (MICER).

Adams DJ, Biggs PJ, Cox T, Davies R, van der Weyden L et al.

Nature genetics 2004;36;8;867-71

PUBMED: 15235602; DOI: 10.1038/ng1388
Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells.

Guo G, Wang W and Bradley A

Nature 2004;429;6994;891-5

PUBMED: 15215866; DOI: 10.1038/nature02653

Functional genetic analysis of mouse chromosome 11.

Kile BT, Hentges KE, Clark AT, Nakamura H, Salinger AP et al.

Nature 2003;425;6953;81-6

PUBMED: 12955145; DOI: 10.1038/nature01865

Mining the mouse genome.

Bradley A

Nature 2002;420;6915;512-4

PUBMED: 12466846; DOI: 10.1038/420512a
Tools for targeted manipulation of the mouse genome.

van der Weyden L, Adams DJ and Bradley A

Physiological genomics 2002;11;3;133-64

PUBMED: 12464689; DOI: 10.1152/physiolgenomics.00074.2002
Cancer: stuck at first base.

van der Weyden L, Jonkers J and Bradley A

Nature 2002;419;6903;127-8

PUBMED: 12226650; DOI: 10.1038/419127a
p53 mutant mice that display early ageing-associated phenotypes.

Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N et al.

Nature 2002;415;6867;45-53

PUBMED: 11780111; DOI: 10.1038/415045a
Induced mitotic recombination: a switch in time.

Adams DJ and Bradley A

Nature genetics 2002;30;1;6-7

PUBMED: 11753379; DOI: 10.1038/ng0102-6

Engineering chromosomal rearrangements in mice.

Yu Y and Bradley A

Nature reviews. Genetics 2001;2;10;780-90

PUBMED: 11584294; DOI: 10.1038/35093564
An SSLP marker-anchored BAC framework map of the mouse genome.

Cai WW, Chow CW, Damani S, Gregory SG, Marra M and Bradley A

Nature genetics 2001;29;2;133-4

PUBMED: 11586294; DOI: 10.1038/ng1001-133
Disruption of an imprinted gene cluster by a targeted chromosomal translocation in mice.

Cleary MA, van Raamsdonk CD, Levorse J, Zheng B, Bradley A and Tilghman SM

Nature genetics 2001;29;1;78-82

PUBMED: 11528397; DOI: 10.1038/ng715
Tbx1 haploinsufficieny in the DiGeorge syndrome region causes aortic arch defects in mice.

Lindsay EA, Vitelli F, Su H, Morishima M, Huynh T et al.

Nature 2001;410;6824;97-101

PUBMED: 11242049; DOI: 10.1038/35065105

Cancer predisposition caused by elevated mitotic recombination in Bloom mice.

Luo G, Santoro IM, McDaniel LD, Nishijima I, Mills M et al.

Nature genetics 2000;26;4;424-9

PUBMED: 11101838; DOI: 10.1038/82548

Allan Bradley | Former Senior Group Leader & Director Emeritus

Bradley, Allan

Publications

Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines.

A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice.

Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours.

Mice deficient for Rb are nonviable and show defects in neurogenesis and haematopoiesis.

Alpha-inhibin is a tumour-suppressor gene with gonadal specificity in mice.

Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2.

p63 is a p53 homologue required for limb and epidermal morphogenesis.

Engineering a mouse balancer chromosome.

Cancer predisposition caused by elevated mitotic recombination in Bloom mice.

Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells.

Requirement of bic/microRNA-155 for normal immune function.

Abnormal behavior in a chromosome-engineered mouse model for human 15q11-13 duplication seen in autism.

A conditional knockout resource for the genome-wide study of mouse gene function.

A resource of vectors and ES cells for targeted deletion of microRNAs in mice.

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.

Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery.

FAMIN Is a Multifunctional Purine Enzyme Enabling the Purine Nucleotide Cycle.

High-throughput discovery of genetic determinants of circadian misalignment.

A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Salmonella Infection.

NPM1c alters FLT3-D835Y localization and signaling in acute myeloid leukemia.

Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming.

PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice.

ATM orchestrates the DNA-damage response to counter toxic non-homologous end-joining at broken replication forks.

PiggyBac Transposon-Based Insertional Mutagenesis in Mice.

SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4.

Repair of double-strand breaks induced by CRISPR-Cas9 leads to large deletions and complex rearrangements.

Inter-homologue repair in fertilized human eggs?

Comprehensive annotation and evolutionary insights into the canine (Canis lupus familiaris) antigen receptor loci.

Evolutionary routes and KRAS dosage define pancreatic cancer phenotypes.

Revealing hidden complexities of genomic rearrangements generated with Cas9.

Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.

A single-copy Sleeping Beauty transposon mutagenesis screen identifies new PTEN-cooperating tumor suppressor genes.

Deletion of the MAD2L1 spindle assembly checkpoint gene is tolerated in mouse models of acute T-cell lymphoma and hepatocellular carcinoma.

Genome-wide transposon screening and quantitative insertion site sequencing for cancer gene discovery in mice.

Disentangling PTEN-cooperating tumor suppressor gene networks in cancer.

High-throughput discovery of novel developmental phenotypes.

Priming HIV-1 broadly neutralizing antibody precursors in human Ig loci transgenic mice.

C13orf31 (FAMIN) is a central regulator of immunometabolic function.

Aneuploid embryonic stem cells exhibit impaired differentiation and increased neoplastic potential.

Single-cell sequencing reveals karyotype heterogeneity in murine and human malignancies.

Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.

Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice.

Chromosome engineering in zygotes with CRISPR/Cas9.

Genome wide conditional mouse knockout resources

CRISPR/Cas9 somatic multiplex-mutagenesis for high-throughput functional cancer genomics in mice.

High-density P300 enhancers control cell state transitions.

A Synergistic Interaction between Chk1- and MK2 Inhibitors in KRAS-Mutant Cancer.

Off-target assessment of CRISPR-Cas9 guiding RNAs in human iPS and mouse ES cells.

A conditional piggyBac transposition system for genetic screening in mice identifies oncogenic networks in pancreatic cancer.

A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer.

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.

Chromosome instability induced by Mps1 and p53 mutation generates aggressive lymphomas exhibiting aneuploidy-induced stress.

Genetic background drives transcriptional variation in human induced pluripotent stem cells.

Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery.

MiR-210 is induced by Oct-2, regulates B cells, and inhibits autoantibody production.

A powerful molecular synergy between mutant Nucleophosmin and Flt3-ITD drives acute myeloid leukemia in mice.

Detailed molecular characterisation of acute myeloid leukaemia with a normal karyotype using targeted DNA capture.

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.

A genetic progression model of Braf(V600E)-induced intestinal tumorigenesis reveals targets for therapeutic intervention.

Histone deacetylase 1 and 2 are essential for normal T-cell development and genomic stability in mice.

Prelamin A causes progeria through cell-extrinsic mechanisms and prevents cancer invasion.

Mouse large-scale phenotyping initiatives: overview of the European Mouse Disease Clinic (EUMODIC) and of the Wellcome Trust Sanger Institute Mouse Genetics Project.

A resource of vectors and ES cells for targeted deletion of microRNAs in mice.

A conditional knockout resource for the genome-wide study of mouse gene function.

Genetic screens using the piggyBac transposon.

A hyperactive piggyBac transposase for mammalian applications.

PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice.

Ectodomains of the LDL receptor-related proteins LRP1b and LRP4 have anchorage independent functions in vivo.

An expanded Oct4 interaction network: implications for stem cell biology, development, and disease.

The use of DNA transposons for cancer gene discovery in mice.

Discovery of candidate disease genes in ENU-induced mouse mutants by large-scale sequencing, including a splice-site mutation in nucleoredoxin.

Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

Abnormal behavior in a chromosome-engineered mouse model for human 15q11-13 duplication seen in autism.

Chromosomal mobilization and reintegration of Sleeping Beauty and PiggyBac transposons.

Transposon-mediated genome manipulation in vertebrates.

Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon.