Andrew leads the Human Gene Editing R&D group at the Wellcome Sanger Institute. After obtaining his PhD at the MRC-LMB with Andrew Travers on chromatin remodelling in heterochromatin formation, his postdoctoral work focused on the role of small RNAs in targeting chromatin modifications with David Baulcombe in Cambridge, and the function of long non-coding RNA molecules with Chris Ponting and Ji-Long Liu at the MRC-FGU in Oxford. Here he was one of the first to develop CRISPR in Drosophila. He then set up Genome Engineering Oxford at the Dunn School of Pathology, and was involved in projects including the production of sgRNA libraries, and development of methods to investigate miRNA target site functionality in vivo. He has subsequently applied genome editing to different species, the modification of the epigenetic and transcriptional status of a cell, and developed methods to improve the efficiency and specificity of the technology. He is continuing to develop genome engineering techniques in human pluripotent stem cells, with a particular interest in understanding regulation of gene expression in development and neurodegenerative disease including the design and application of a variety of pooled and arrayed CRISPR screening approaches.
Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes.
Methods in molecular biology (Clifton, N.J.) 2019;1961;153-183
Predicting the mutations generated by repair of Cas9-induced double-strand breaks.
Nature biotechnology 2018
In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering.
Nature communications 2017;8;1;2109
Alpha-synuclein induces the unfolded protein response in Parkinson's disease SNCA triplication iPSC-derived neurons.
Human molecular genetics 2017;26;22;4441-4450
Editing the genome of hiPSC with CRISPR/Cas9: disease models.
Mammalian genome : official journal of the International Mammalian Genome Society 2017;28;7-8;348-364
Precision Modulation of Neurodegenerative Disease-Related Gene Expression in Human iPSC-Derived Neurons.
Scientific reports 2016;6;28420
A genome-wide CRISPR library for high-throughput genetic screening in Drosophila cells.
Journal of genetics and genomics = Yi chuan xue bao 2015;42;6;301-9
Understanding functional miRNA-target interactions in vivo by site-specific genome engineering.
Nature communications 2014;5;4640
Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9.
Biology open 2014;3;1;42-9
Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system.
Cell reports 2013;4;1;220-8