Wellcome Sanger Institute sequences reference genomes of 3,000 dangerous bacteria

Gonorrhoea, deadly plague and dysentery among collection of bacterial genomes now publicly available through collaboration with Pacific Biosciences

Wellcome Sanger Institute sequences reference genomes of 3,000 dangerous bacteria

Wellcome Sanger Institute sequences reference genomes of 3,000 dangerous bacteria

The genomes of more than 3,000 bacteria, including some of the world’s most dangerous, have been sequenced by researchers at the Wellcome Sanger Institute in collaboration with Pacific Biosciences (PacBio). Infecting tens of millions of people worldwide every year, these bacteria have been collected by the National Collection of Type Cultures (NCTC) and include deadly strains of plague, dysentery and cholera.

Plague_facts_4web.jpg

By decoding the DNA, researchers will be able to better understand these diseases and how they become resistant to antibiotics. The publicly available genomic maps could also lead to the development of new diagnostic tests, vaccines or treatments.

Set up in 1920, the NCTC is the longest established collection of bacteria in the world. With more than 5,500 species of bacteria so far, the NCTC is also one of the world’s largest collections of clinically relevant bacteria. It is used extensively by researchers who are comparing historical and modern strains to advance global knowledge about the epidemiology, virulence, prevention and treatment of infectious diseases.

Antibiotic resistance is a significant problem globally and the collection includes some of the most important known drug-resistant bacteria. These include tuberculosis, one of the top ten causes of death worldwide, infecting 10.4 million and killing 1.7 million people in 2016 alone*, and gonorrhoea, the sexually transmitted disease that infects 78 million people a year** and is now becoming extremely difficult to treat. The NCTC also contains samples of methicillin-resistant Staphylococcus aureus (MRSA), which is resistant to multiple antibiotics and which can cause life-threatening infections in hospitals.

The genetic study of these strains will help researchers to understand the mechanisms of antibiotic resistance, and to look for any cracks in their armour to enable treatment.

MRSA_facts_body.jpg

All ‘type strains’ of bacteria in the collection, the first strains that describe the species and are used to classify them, were sequenced as part of this initiative. The genome sequences of these highly valuable strains are fundamental for developing ways to identify specific infections in people, including tests diagnosing bacterial infections in the field to rapidly identify the source of an outbreak and help contain infections.

Amongst the many historically important strains in the collection are 16 deposited by penicillin discoverer Alexander Fleming, including a sample taken from his own nose. Also notable is the first bacteria to be deposited in the NCTC: A strain of dysentery-causing Shigella flexneri that was isolated in 1915 from a soldier in the trenches of World War 1***.

“Historical collections such at the NCTC are of enormous value in understanding current pathogens. Knowing very accurately what bacteria looked like before and during the introduction of antibiotics and vaccines, and comparing them to current strains from the same collection, shows us how they have responded to these treatments. This in turn helps us develop new antibiotics and vaccines. PacBio’s comprehensive DNA sequencing enables deep genomic analyses, and we are happy to be partnering with them for this important project.”

Dr Julian Parkhill from the Wellcome Sanger Institute

Gonorrhoea_facts4_web.jpg

“The high-quality genomic maps enabled by SMRT® Sequencing allow an unprecedented understanding of these bacteria. We are delighted to be chosen by institutions like Wellcome Sanger Institute to help create such essential resources for the scientific and public health communities.”

Jonas Korlach, Ph.D., Chief Scientific Officer of PacBio

“This resource is a vital tool for public health and by sequencing the bacteria, we have made the NCTC collection ready for the 21st century so that the research community can track and understand the bacteria. With this collection, we are providing tools for tracing infections, and identifying outbreaks of resistant bacteria, transforming public health in the UK.”

Dr Julie Russell, Head of Culture Collections, which is operated by the National Infection Service of Public Health England

Going forward, all the bacterial species in the NCTC collection will be sequenced as they are collected. Researchers can order bacterial strains from the NCTC website at: https://www.phe-culturecollections.org.uk/collections/nctc.aspx. Full information about each strain, including the DNA sequences, are available at EMBL-EBI: https://www.ebi.ac.uk/ena/data/view/PRJEB6403.

Notes to Editors

*WHO figures http://www.who.int/en/news-room/fact-sheets/detail/tuberculosis

** WHO figures Each year, an estimated 78 million people are infected with gonorrhoea*.

http://www.who.int/en/news-room/detail/07-07-2017-antibiotic-resistant-gonorrhoea-on-the-rise-new-drugs-needed

*** By sequencing a strain of dysentery-causing Shigella flexneri that was isolated in 1915 from a soldier in the trenches of World War 1 and comparing it to three other strains isolated in 1954, 1984 and 2002, researchers were able to determine an evolutionary pattern of the disease and learn about its virulence and antibiotic resistance.

https://www.sanger.ac.uk/news/view/2014-11-07-world-war-i-soldier-helps-in-fight-against-dysentery

https://www.youtube.com/watch?v=u-fbd9JpiMs

Funding:

This project was supported by Wellcome and Pacific Biosciences.

Forward-Looking Statements

All statements in this press release that are not historical are forward-looking statements, including, among other things, statements relating to the expected benefits of the collaboration between Pacific Biosciences and the Wellcome Sanger Institute, the suitability of products or technologies for particular applications, future availability, uses, quality or performance of, or benefits of using, products or technologies, and other future events. You should not place undue reliance on forward-looking statements because they involve known and unknown risks, uncertainties, changes in circumstances and other factors that are, in some cases, beyond Pacific Biosciences’ control and could cause actual results to differ materially from the information expressed or implied by forward-looking statements made in this press release. Factors that could materially affect actual results can be found in Pacific Biosciences’ most recent filings with the Securities and Exchange Commission, including Pacific Biosciences’ most recent reports on Forms 8-K, 10-K and 10-Q, and include those listed under the caption “Risk Factors.” Pacific Biosciences undertakes no obligation to revise or update information in this press release to reflect events or circumstances in the future, even if new information becomes available.

Selected Websites
MRSAVideoMRSA
This animation shows you how antibiotic resistant strains of bacteria, such as MRSA, can develop and spread, particularly in hospitals.

Tracking ‘superbugs’StoriesTracking ‘superbugs’
Infectious diseases can spread quickly in a hospital environment, particularly if the pathogen that causes the disease is resistant to the drugs relied on to fight it. Genomics may be able to help track these resistant pathogens and nip them in the bud before they become widespread…

What are Staphylococcal infections?FactsWhat are Staphylococcal infections?
Staphylococcal infections are a group of infections caused by the bacterium Staphylococcus. Infection causes a range of symptoms, from skin conditions to food poisoning.

Contact the Press Office

Dr Samantha Wynne, Media Officer

Tel +44 (0)1223 492 368

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CB10 1SA,
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