PlasmoGEM is a non-profit, open-access malaria research resource, providing tools for the manipulation of Plasmodium genomes, and using them to carry out large-scale research projects. These tools currently include:
We have produced large numbers of DNA vectors for the targeted manipulation of P. berghei genes. Resources available currently include:
A large-insert (6-10kb) P. berghei genomic library covering >90% of P. berghei genes
A library of artificial chromosomes covering >3500 P. berghei genes for use in complementation or over-expression studies.
Gene targeting vectors for the disruption of >2600 P. berghei genes and epitope tagging of >400 genes. We are currently expanding our knockout library to attempt to cover the entire genome, excluding large multi-gene families such as birs.
Blood-stage growth phenotypes for >2,600 genes, generated using barcode sequencing after pooled transfection with our gene targeting knock out vectors
All the tools as well as phenotypic data currently available can be found here.
PlasmoGEM is currently generating vectors for Cas9 and gRNA expression (pDC2) as well as barcoded homologous repair (HR) templates (pCC1) for 200-300 P.falciparum genes.
PlasmoGEM is currently generating vectors for Cas9 and gRNA expression (pK-U6Cas9) as well as barcoded homologous repair (HR) templates by PCR for 400-500 P. knowlesi genes.
The Cellular Generation and Phenotyping (CGaP) core facility provides central cell biology support to the Sanger Institute. CGaP takes a unique approach at the institute by partnering with faculty groups in order to deliver the scale-up of existing protocols to facilitate 'Science at Scale'. We function as a contract research group for the institute, running multiple, distinct cell biology based projects. The facility has expertise in cell derivation from primary tissue, iPSC and organoid derivation, cellular differentiation, CRISPR library screening, functional bioassays, phenotypic assays and end point analysis (e.g. Immunocytochemistry).
Marcus Lee’s group is interested in the molecular basis of drug resistance in the human malaria parasite Plasmodium falciparum, and in developing molecular genetics tools to interrogate gene function in this important pathogen.
Julian Rayner's group investigates the molecular details of human-parasite interactions during the P. falciparum blood stages, with a particular focus on large-scale experimental approaches to understanding erythrocyte invasion.
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We are using genomics to get at important problems in infectious disease, with a strong desire to translate this into tools for disease control and elimination, but we are also at the forefront of basic research into microbial ecology, evolutionary genetics and the biology of parasitism.
Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes.
Bushell E, Gomes AR, Sanderson T, Anar B, Girling Get al.