CAROL is a combined functional annotation score of non-synonymous coding variants.

A major challenge in interpreting whole-exome data is predicting which of the discovered variants are deleterious or neutral.

To address this question in silico, we have developed a score called Combined Annotation scoRing toOL (CAROL), which combines information from two bioinformatics tools: PolyPhen-2 and SIFT, in order to improve the prediction of the effect of non-synonymous coding variants. The combination of annotation tools can help improve automated prediction of whole-genome/exome non-synonymous variant functional consequences.

Download and Installation

System requirements

The software should run on any UNIX or GNU/Linux system.

Download CAROL

After downloading the files, please read the CAROL_README.txt file to set up and run the files.

License and Citation

This is the licensing and citation area. Describe the terms of licence for using the software - such as creative commons.

Also give information on how you wish the software or database to be credited/cited in research papers and other literature.


If you have any problems running CAROL, then please contact either: Margarida Lopes or Eleftheria Zeggini.

It is recommended that you contact the author of CAROL, Margarida Lopes regarding bugs or problems running the script.


External Contributors


  • A combined functional annotation score for non-synonymous variants.

    Lopes MC, Joyce C, Ritchie GR, John SL, Cunningham F et al.

    Human heredity 2012;73;1;47-51

Tool Type