The High Throughput RNA and LCMB Sequencing team within DNA Pipelines Operations is a high throughput Illumina® library creation team which specialises in producing libraries quickly and at scale from RNA and laser capture microdissection (LCMB)-derived material. Once library preparation is complete the samples are sent to be sequenced on the Wellcome Sanger Institute’s collection of Illumina® instruments including the NovaSeq 6000, HiSeq X, Hiseq 4000, Hiseq 2500 and MiSeq.
The library preparation services provided by our group include:
• stranded mRNA libraries
• globin depleted RNA libraries
• rRNA depleted RNA libraries
• low input (10ng) RNA libraries
• Laser Capture Microdissection Biopsy (LCMB) libraries