This person is a member of Sanger Institute Alumni.
My current research involves using hIPSC to develop alternative in vitro models to study host-pathogen interactions. Specifically, I differentiate hIPSCs to macrophages to study its response to pathogens, including Salmonella and Chlamydia. To study the response of the macrophages, I use various techniques, including flow cytometry, cellular imagining (Cellomics, fluroescent microscopy, electron microscopy), RNA sequencing, and proteomics. I am also using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) gene editing technology to generate genome-wide CRISPR-Cas9 knockout mutant libraries in a human macrophage line to identify identify novel host factors involved in macrophage resistance to pathogens and toxins.