My current research involves using hIPSC to develop alternative in vitro models to study host-pathogen interactions. Specifically, I differentiate hIPSCs to macrophages to study its response to pathogens, including Salmonella and Chlamydia. To study the response of the macrophages, I use various techniques, including flow cytometry, cellular imagining (Cellomics, fluroescent microscopy, electron microscopy), RNA sequencing, and proteomics. I am also using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) gene editing technology to generate genome-wide CRISPR-Cas9 knockout mutant libraries in a human macrophage line to identify identify novel host factors involved in macrophage resistance to pathogens and toxins.
Exploiting induced pluripotent stem cell-derived macrophages to unravel host factors influencing Chlamydia trachomatis pathogenesis.
Nature communications 2017;8;15013
Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function.
Scientific reports 2015;5;8908
Induced pluripotent stem cell derived macrophages as a cellular system to study salmonella and other pathogens.
PloS one 2015;10;5;e0124307
The sensor kinase CbrA is a global regulator that modulates metabolism, virulence, and antibiotic resistance in Pseudomonas aeruginosa.
Journal of bacteriology 2011;193;4;918-31
Antibiotic resistance in Pseudomonas aeruginosa biofilms: towards the development of novel anti-biofilm therapies.
Journal of biotechnology 2014;191;121-30
Mucin promotes rapid surface motility in Pseudomonas aeruginosa.
Multifunctional cationic host defence peptides and their clinical applications.
Cellular and molecular life sciences : CMLS 2011;68;13;2161-76
Therapeutic potential of host defense peptides in antibiotic-resistant infections.
Current pharmaceutical design 2012;18;6;807-19
Swarming of Pseudomonas aeruginosa is controlled by a broad spectrum of transcriptional regulators, including MetR.
Journal of bacteriology 2009;191;18;5592-602
Requirement of the Pseudomonas aeruginosa CbrA sensor kinase for full virulence in a murine acute lung infection model.
Infection and immunity 2014;82;3;1256-67