Ms Anne Maria McLaren-Douglas, BSc | Senior Research Assistant

This person is a member of Sanger Institute Alumni.

McLaren-Douglas, Anne Maria

Anne's main role intially with the Cancer Translation Team was to manage the operation of the lab and to meet scientific needs of research. The goals were to implement and maintain high operating standards throughout the project and to deliver complex scientific projects such as the Organoid Project. This involved taking a lead role in the development of a new organoid screening facility.

This included :-

Interacting with external organisations to acquire and share new technologies and cultures, and the development and optimisation of conditions for organiod growth

The development of a novel screening assays to measure drug response in 3D organoid culture.

The development of protocols, for the derivation of organoids.

And finally to train and transfer knowledge of the organoid process to colleagues.

I have been a part of a pilot study using the 3D cancer organoid models, this unique cell format was used to investigate their effectiveness to study colon cancer. A panel of 22 colon cancer organoids were derived from the tumours of 20 patients and they were found to authentically capture the genomics of the primary tumour as well as much of the genetic scope of colon cancer

Publications

  • Systematic identification of genomic markers of drug sensitivity in cancer cells.

    Garnett MJ, Edelman EJ, Heidorn SJ, Greenman CD, Dastur A et al.

    Nature 2012;483;7391;570-5

  • Prospective derivation of a living organoid biobank of colorectal cancer patients.

    van de Wetering M, Francies HE, Francis JM, Bounova G, Iorio F et al.

    Cell 2015;161;4;933-45

  • Prospective derivation of a living organoid biobank of colorectal cancer patients.

    van de Wetering M, Francies HE, Francis JM, Bounova G, Iorio F et al.

    Cell 2015;161;4;933-45

  • Systematic identification of genomic markers of drug sensitivity in cancer cells.

    Garnett MJ, Edelman EJ, Heidorn SJ, Greenman CD, Dastur A et al.

    Nature 2012;483;7391;570-5

  • Use of Cryopreserved Cells for Enabling Greater Flexibility in Compound Profiling

    M.J.Wigglesworth et al. et al.

    Journal of biomolecular screening 2008

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Anne's Timeline
2012

The Organoid Project Sanger- Investigated the option of using a 3D cell model as opposed to 2D cell line in a gel matrix media

2009

Cancer Translation team Sanger- Managed the screen team

2002

GSK Screening Compound Team- Ran selected screening programs against bespoke targets

1992

Post with GSK Natural products Group - Extracting natural compounds from Bacteria and virus on complex media