David Goulding | Electron Microscope Manager

Goulding, David

David's role is to provide imaging support for team 15 and Pathogen Variation using electron and advanced light microscopy. His expertise lies in ultrastructure techniques, including fine structure immunocytochemistry, to study interactions between host cells and microorganisms. The microscope suite in Gordon Dougan's lab houses TEM, SEM, confocal and microinjection microscopes with a variety of analysis software platforms.


  • Interaction of Salmonella enterica Serovar Typhimurium with Intestinal Organoids Derived from Human Induced Pluripotent Stem Cells.

    Forbester JL, Goulding D, Vallier L, Hannan N, Hale C et al.

    Infection and immunity 2015;83;7;2926-34

  • An O antigen capsule modulates bacterial pathogenesis in Shigella sonnei.

    Caboni M, Pédron T, Rossi O, Goulding D, Pickard D et al.

    PLoS pathogens 2015;11;3;e1004749

  • The Hd, Hj, and Hz66 flagella variants of Salmonella enterica serovar Typhi modify host responses and cellular interactions.

    Schreiber F, Kay S, Frankel G, Clare S, Goulding D et al.

    Scientific reports 2015;5;7947

  • Spinster homolog 2 (spns2) deficiency causes early onset progressive hearing loss.

    Chen J, Ingham N, Kelly J, Jadeja S, Goulding D et al.

    PLoS genetics 2014;10;10;e1004688

  • Juno is the egg Izumo receptor and is essential for mammalian fertilization.

    Bianchi E, Doe B, Goulding D and Wright GJ

    Nature 2014;508;7497;483-7

  • Molecular characterization of the viaB locus encoding the biosynthetic machinery for Vi capsule formation in Salmonella Typhi.

    Wetter M, Goulding D, Pickard D, Kowarik M, Waechter CJ et al.

    PloS one 2012;7;9;e45609

  • IFITM3 restricts the morbidity and mortality associated with influenza.

    Everitt AR, Clare S, Pertel T, John SP, Wash RS et al.

    Nature 2012;484;7395;519-23

  • A Salmonella Typhimurium-Typhi genomic chimera: a model to study Vi polysaccharide capsule function in vivo.

    Jansen AM, Hall LJ, Clare S, Goulding D, Holt KE et al.

    PLoS pathogens 2011;7;7;e1002131

  • Troponin T is essential for sarcomere assembly in zebrafish skeletal muscle.

    Ferrante MI, Kiff RM, Goulding DA and Stemple DL

    Journal of cell science 2011;124;Pt 4;565-77

  • Distinctive profiles of infection and pathology in hamsters infected with Clostridium difficile strains 630 and B1.

    Goulding D, Thompson H, Emerson J, Fairweather NF, Dougan G and Douce GR

    Infection and immunity 2009;77;12;5478-85

Goulding, David