Lung Cancer and melanoma laid bare

First comprehensive analysis of two cancer genomes

Lung Cancer and melanoma laid bare

lung-melanoma-circos.jpg
From outside to inside: insertions or deletions (green); coding substitutions as numbers per 10 million (orange charts); coding substitutions (grey, purple, red and black marks); copy number (blue); deletions (red); rearrangements (green or purple)

Research teams led by the Wellcome Trust Sanger Institute announce the first comprehensive analyses of cancer genomes.

All cancers are caused by mutations in the DNA of cancer cells which are acquired during a person's lifetime. The studies, of a malignant melanoma and a lung cancer, reveal for the first time essentially all the mutations in the genomes of two cancers.

Lung cancer causes around one million deaths worldwide each year: almost all are associated with smoking. The number of mutations found suggest that a typical smoker would acquire one mutation for every 15 cigarettes smoked.

Although malignant melanoma comprises only 3 per cent of skin cancer cases, it is the cause of three out of four skin cancer deaths. The melanoma genome contained more than 30,000 mutations that carried a record of how and when they occurred during the patient's life.

"These are the two main cancers in the developed world for which we know the primary exposure. For lung cancer, it is cigarette smoke and for malignant melanoma it is exposure to sunlight. With these genome sequences, we have been able to explore deep into the past of each tumour, uncovering with remarkable clarity the imprints of these environmental mutagens on DNA, which occurred years before the tumour became apparent."​

"We can also see the desperate attempts of our genome to defend itself against the damage wreaked by the chemicals in cigarette smoke or the damage from ultraviolet radiation. Our cells fight back furiously to repair the damage, but frequently lose that fight."

Professor Mike Stratton, from the Cancer Genome Project at the Wellcome Trust Sanger Institute

The studies used powerful new DNA sequencing technologies to decode completely the genome of both tumour tissue and normal tissue from a lung cancer and a malignant melanoma patient. By comparing the genome sequence from the cancer to the genome from healthy tissue they could pick up the changes specific to the cancer. The studies are the first to produce comprehensive genome-wide descriptions of all classes of mutation, producing rich accounts of the genetic changes in the development of the two cancers.

"In the melanoma sample, we can see sunlight's signature writ large in the genome. However, with both samples, because we have produced essentially complete catalogues, we can see other, more mysterious processes acting on the DNA. Indeed, somewhere amongst the mutations we have found lurk those that drive the cells to become cancerous. Tracking them down will be our major challenge for the next few years."

Dr Andy Futreal, from the Wellcome Trust Sanger Institute

The lung cancer genome contained more than 23,000 mutations, the melanoma more than 33,000. Identifying the causative mutations among the large number found poses a challenge, but the complete genome sequences mean, that for the first time, that challenge can be met.

"Nearly 10 years on, we are still reaping the benefit from the first human genome sequence and we have much still to do to get to grips with these new disrupted landscapes of cancer genomes. But the knowledge we extract over the next few years will have major implications for treatment. By identifying all the cancer genes we will be able to develop new drugs that target the specific mutated genes and work out which patients will benefit from these novel treatments."

Dr Peter Campbell from the Wellcome Trust Sanger Institute

A complete genome catalogue for each patient would be expected to help select between treatments and to direct treatment in the most efficient and cost-effective way. The Sanger Institute is already working with researchers at Massachusetts General Hospital on a large scale project to tie genetic changes in cancers to their responses to anticancer treatments.

"We want to drive healthcare through better understanding of the biology of disease. Previous outcomes from our Cancer Genome Project are already being fed into clinical trials, and these remarkable new studies further emphasise the extraordinary scientific insights and benefits for patients that accrue from studying the genome of cancer cells."

"This is the first glimpse of the future of cancer medicine, not only in the laboratory, but eventually in the clinic. The findings from today will feed into knowledge, methods and practice in patient care."

Sir Mark Walport, Director of the Wellcome Trust

The human genome is large. Moreover, there are more than one hundred different types of cancer and sequencing genomes is expensive. To ensure that thousands of cancers ultimately are sequenced in the same way as these two, the International Cancer Genome Consortium has been established, on the model of the Human Genome project itself to coordinate cancer genome sequencing across the globe.

These catalogues of mutations across the broad diversity of cancer types will provide powerful insights into the biology of cancer and will be the foundation for understanding cancer causation and improving prevention, detection and treatment.

  • For more information about the study into small cell lung cancer, please read the press release: One mutation every day
  • For more information about the study into malignant melanoma, please read the press release: Signatures of sunlight

Cancer is a leading cause of death worldwide. The disease accounted for 7.4 million deaths (or around 13 per cent of all deaths worldwide) in 2004. The main types of cancer leading to overall cancer mortality each year are:

  • lung (1.3 million deaths/year)
  • stomach (803,000 deaths)
  • colorectal (639,000 deaths)
  • liver (610,000 deaths)
  • breast (519,000 deaths)

http://www.who.int/mediacentre/factsheets/fs297/en/index.html

Notes to Editors
Publications
  • A small-cell lung cancer genome with complex signatures of tobacco exposure.

    Pleasance ED, Stephens PJ, O'Meara S, McBride DJ, Meynert A et al.

    Nature 2010;463;7278;184-90

  • A comprehensive catalogue of somatic mutations from a human cancer genome.

    Pleasance ED, Cheetham RK, Stephens PJ, McBride DJ, Humphray SJ et al.

    Nature 2010;463;7278;191-6

Funding

This work was supported by the Wellcome Trust.
Support for individual researchers was from the Kay Kendall Leukaemia Fund, Human Frontiers Science Program and the National Cancer Institute.

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