Plasmid genomes - data download
Miscellaneous plasmids
The Sanger Institute Pathogen Genomics group is sequencing a number of plasmids from various sources. Sequence data for each project will be made available for download (FTP site) and searching (BLAST server) once assemblies have begun. The annotation will be made available upon publication.
Plasmid Information
| Plasmid | Source | Size | Phenotype | Status | Data |
|---|---|---|---|---|---|
| pBToxis* | Bacillus thuringiensis | 137 kb | insecticidal toxins | Published | FTP site EMBL/GenBank BLAST server |
| pCoo** | Enterotoxigenic E. coli | 98.4 kb | CS1 pilus | Published | FTP site EMBL/GenBank BLAST server |
| R478*** | Serratia marcescens | 286kb | Antibiotic resistance | Published | FTP site EMBL/GenBank BLAST server |
| pHag1/pHag2 | Bacteroides fragilis strain WI1 | 5.6 kb/9.9 kb | Antibiotic resistance | Finished | FTP site |
* In collaboration with Dr. Colin Berry of the School of Biosciences, Cardiff University and Dr Arieh Zaritsky of the Department of Life Sciences, Ben-Gurion University of the Negev, Israel.
** In collaboration with June Scott of the Dept of Microbiology and Immunology, Emory University, USA.
*** In collaboration with Diane Taylor of the Department of Medical Microbiology and Immunology, University of Alberta, Canada.
E. coli reference plasmid
In collaboration with Christopher Thomas (University of Birmingham), John Wain (Sanger Institute) and the broader plasmid biology community (with advisors Anne Summers, Athens Georgia, USA and Ron Skurray, Sydney, Australia) we are sequencing reference plasmids for each of the Escherichia coli incompatibility groups for which no complete sequence exists, along with equivalent plasmids from modern clinical isolates. These are all listed below. If you have a plasmid that might be interesting to sequence in this context please contact Christopher Thomas. The sequencing of plasmid pVL501 is in collaboration with Jonathan Fletcher (University of Bradford).
Plasmid Information
| Plasmid | Size | Inc group | NCTC# | Resistances | Status | Data |
|---|---|---|---|---|---|---|
| RA1 | 129 kb | IncA/C | 50073 | Su Tc | Planned | – |
| R667 | 168 kb | IncA/C | 50463 | Ap Su | Finished | Sequence |
| TP113 | 86 kb | IncB=O | 50084 | Km Hg | Finished | Sequence |
| TP114 | 62 kb | IncIbeta(2) | 50085 | Km | Planned | – |
| R387 | 80 kb | IncK | 50022 | Cm Sm | Finished | Sequence |
| R471 | 78 kb | IncL/M | 50036 | Ap Hg | Finished | Sequence |
| pIP135 | 68 kb | IncL/M | 50107 | Gm Sm Su Tc Hg | Finished | Sequence |
| N3 | 50 kb | IncN | 50124 | Sm Sp Su Tc Hg [HspII] | In Finishing | – |
| R753 | 102 kb | IncV | 50521 | Ap Cm Sm Su | Planned | – |
| R7K | 30 kb | IncW | 50006 | Ap Sm Sp | Published | Sequence |
| pIP71 | 68 kb | Com9 | 50106 | Ap Cm Sm Sp Su Tc | Finished | Sequence |
| R6K | 39 kb | IncX | 50005 | Ap Sm | Finished | Sequence |
| R485 | 54 kb | IncX | 50040 | Su | Planned | – |
| pHH1508a | 208 kb | IncH | – | Sm Sp Tp Tel Phi | In Finishing | – |
| MIP233 | 231 kb | IncHI3 | – | Scr Phi Tel | In Finishing | – |
| pLV501 | 96.5 kb | IncFII | – | Cm Km Tc Hg | Finished | Sequence |
Legend
Ap – ampicillin; Su – sulphonamide; Gm – gentamycin; Cm – chloramphenicol; Tc – tetracycline; Tp – trimethoprim; Sm – streptomycin; Sp – spectinomycin; Km – kanamycin; Hg – mercury resistance; Tel – tellurite resistance; Phi – resistance to various bacteriophages; Scr – sucrose fermentation; [ ] square brackets – restriction-modification system
Related Links
Data use
This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.