ACT is a Java application for displaying pairwise comparisons between two or more DNA sequences. It can be used to identify and analyse regions of similarity and difference between genomes and to explore conservation of synteny, in the context of the entire sequences and their annotation. It can read complete EMBL, GENBANK and GFF entries or sequences in FASTA or raw format.
Two separate ACT-related web applications have been developed.
- WebACT was developed at Imperial College, and allows users to generate comparisons from their sequence files or use a set of pre-computed comparisons. All comparison files can be downloaded for local use or launched in ACT.
- DoubleACT was developed by Anthony Underwood and Jonathan Green at the Public Public Health England and allows you to paste or upload sequences to generate ACT comparison files.