Wright Group | Cell Surface Signalling Laboratory

Wright Group | Cell Surface Signalling Laboratory

Wright Group

wright-group.jpgSanger Institute, Genome Research Limited
Wright Group

Our Research and Approach

The Cell Surface Signalling Laboratory is interested in identifying new therapeutic targets for both genetic and infectious diseases by using large-scale systematic approaches to discover receptor-ligand interactions that are essential for cellular recognition processes. Molecular interactions involved in cellular recognition events are typically very weak (often having monomeric half-lives of just fractions of a second) and so we have developed bespoke approaches that require creating highly avid soluble recombinant proteins to detect these fleeting binding events. The laboratory has identified interactions that are essential for basic biological events of fundamental importance which notably include sperm-egg recognition in mammals, and the invasion of erythrocytes by the blood stage of Plasmodium falciparum - the parasite responsible for most clinical cases of the deadly infectious diseases, malaria.

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People

Wright, Gavin
Dr Gavin J Wright
Group Leader

Gavin is interested in the molecular basis of cellular recognition events. The goals of his research are to obtain a mechanistic understanding of how cells interact both within our own bodies and with pathogens. Ultimately, this knowledge will inform the rational design of intervention strategies for the treatment and prevention of genetic and infectious diseases.

Key Projects, Collaborations, Tools & Data

Research Programmes

Partners and Funders

Internal Partners

Publications

  • Juno is the egg Izumo receptor and is essential for mammalian fertilization.

    Bianchi E, Doe B, Goulding D and Wright GJ

    Nature 2014;508;7497;483-7

  • RH5-Basigin interaction plays a major role in the host tropism of Plasmodium falciparum.

    Wanaguru M, Liu W, Hahn BH, Rayner JC and Wright GJ

    Proceedings of the National Academy of Sciences of the United States of America 2013;110;51;20735-40

  • A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins.

    Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K et al.

    Molecular & cellular proteomics : MCP 2013;12;12;3976-86

  • Biochemical analysis of the Plasmodium falciparum erythrocyte-binding antigen-175 (EBA175)-glycophorin-A interaction: implications for vaccine design.

    Wanaguru M, Crosnier C, Johnson S, Rayner JC and Wright GJ

    The Journal of biological chemistry 2013;288;45;32106-17

  • Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum.

    Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M et al.

    Nature 2011;480;7378;534-7

  • Jamb and jamc are essential for vertebrate myocyte fusion.

    Powell GT and Wright GJ

    PLoS biology 2011;9;12;e1001216

  • DeltaC and DeltaD interact as Notch ligands in the zebrafish segmentation clock.

    Wright GJ, Giudicelli F, Soza-Ried C, Hanisch A, Ariza-McNaughton L and Lewis J

    Development (Cambridge, England) 2011;138;14;2947-56

  • Construction of a large extracellular protein interaction network and its resolution by spatiotemporal expression profiling.

    Martin S, Söllner C, Charoensawan V, Adryan B, Thisse B et al.

    Molecular & cellular proteomics : MCP 2010;9;12;2654-65

  • A cell surface interaction network of neural leucine-rich repeat receptors.

    Söllner C and Wright GJ

    Genome biology 2009;10;9;R99

  • Large-scale screening for novel low-affinity extracellular protein interactions.

    Bushell KM, Söllner C, Schuster-Boeckler B, Bateman A and Wright GJ

    Genome research 2008;18;4;622-30

  • Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function.

    Wright GJ, Puklavec MJ, Willis AC, Hoek RM, Sedgwick JD et al.

    Immunity 2000;13;2;233-42

  • P113 is a merozoite surface protein that binds the N terminus of Plasmodium falciparum RH5.

    Galaway F, Drought LG, Fala M, Cross N, Kemp AC et al.

    Nature communications 2017;8;14333

  • EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality.

    Cayuso J, Dzementsei A, Fischer JC, Karemore G, Caviglia S et al.

    Developmental cell 2016;39;3;316-328

  • Sperm Meets Egg: The Genetics of Mammalian Fertilization.

    Bianchi E and Wright GJ

    Annual review of genetics 2016

  • Binding of Plasmodium falciparum Merozoite Surface Proteins DBLMSP and DBLMSP2 to Human Immunoglobulin M Is Conserved among Broadly Diverged Sequence Variants.

    Crosnier C, Iqbal Z, Knuepfer E, Maciuca S, Perrin AJ et al.

    The Journal of biological chemistry 2016;291;27;14285-99

  • The structure of sperm Izumo1 reveals unexpected similarities with Plasmodium invasion proteins.

    Nishimura K, Han L, Bianchi E, Wright GJ, de Sanctis D and Jovine L

    Current biology : CB 2016

  • Divergent evolution of vitamin B9 binding underlies Juno-mediated adhesion of mammalian gametes.

    Han L, Nishimura K, Sadat Al Hosseini H, Bianchi E, Wright GJ and Jovine L

    Current biology : CB 2016;26;3;R100-1

  • A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals New Vaccine Candidates and Protein-Protein Interactions.

    Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM and Rayner JC

    PLoS neglected tropical diseases 2015;9;12;e0004264

  • Processing of Plasmodium falciparum Merozoite Surface Protein MSP1 Activates a Spectrin-Binding Function Enabling Parasite Egress from RBCs.

    Das S, Hertrich N, Perrin AJ, Withers-Martinez C, Collins CR et al.

    Cell host & microbe 2015;18;4;433-44

  • P-selectin is a host receptor for Plasmodium MSP7 ligands.

    Perrin AJ, Bartholdson SJ and Wright GJ

    Malaria journal 2015;14;238

  • A Human Platelet Receptor Protein Microarray Identifies the High Affinity Immunoglobulin E Receptor Subunit α (FcεR1α) as an Activating Platelet Endothelium Aggregation Receptor 1 (PEAR1) Ligand.

    Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF and Wright GJ

    Molecular & cellular proteomics : MCP 2015;14;5;1265-74

  • Cross-species fertilization: the hamster egg receptor, Juno, binds the human sperm ligand, Izumo1.

    Bianchi E and Wright GJ

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2015;370;1661;20140101

  • Revealing the sequence and resulting cellular morphology of receptor-ligand interactions during Plasmodium falciparum invasion of erythrocytes.

    Weiss GE, Gilson PR, Taechalertpaisarn T, Tham WH, de Jong NW et al.

    PLoS pathogens 2015;11;2;e1004670

  • A PfRH5-based vaccine is efficacious against heterologous strain blood-stage Plasmodium falciparum infection in aotus monkeys.

    Douglas AD, Baldeviano GC, Lucas CM, Lugo-Roman LA, Crosnier C et al.

    Cell host & microbe 2015;17;1;130-9

  • A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining.

    Staudt N, Müller-Sienerth N, Fane-Dremucheva A, Yusaf SP, Millrine D and Wright GJ

    Biochemical and biophysical research communications 2015;456;1;527-33

  • The LRR receptor Islr2 is required for retinal axon routing at the vertebrate optic chiasm.

    Panza P, Sitko AA, Maischein HM, Koch I, Flötenmeyer M et al.

    Neural development 2015;10;23

  • New antigens for a multicomponent blood-stage malaria vaccine.

    Osier FH, Mackinnon MJ, Crosnier C, Fegan G, Kamuyu G et al.

    Science translational medicine 2014;6;247;247ra102

  • Juno is the egg Izumo receptor and is essential for mammalian fertilization.

    Bianchi E, Doe B, Goulding D and Wright GJ

    Nature 2014;508;7497;483-7

  • Development of an antigen microarray for high throughput monoclonal antibody selection.

    Staudt N, Müller-Sienerth N and Wright GJ

    Biochemical and biophysical research communications 2014;445;4;785-90

  • Naturally acquired antibodies specific for Plasmodium falciparum reticulocyte-binding protein homologue 5 inhibit parasite growth and predict protection from malaria.

    Tran TM, Ongoiba A, Coursen J, Crosnier C, Diouf A et al.

    The Journal of infectious diseases 2014;209;5;789-98

  • Plasmodium falciparum erythrocyte invasion: combining function with immune evasion.

    Wright GJ and Rayner JC

    PLoS pathogens 2014;10;3;e1003943

  • Cloning of recombinant monoclonal antibodies from hybridomas in a single mammalian expression plasmid.

    Müller-Sienerth N, Crosnier C, Wright GJ and Staudt N

    Methods in molecular biology (Clifton, N.J.) 2014;1131;229-40

  • RH5-Basigin interaction plays a major role in the host tropism of Plasmodium falciparum.

    Wanaguru M, Liu W, Hahn BH, Rayner JC and Wright GJ

    Proceedings of the National Academy of Sciences of the United States of America 2013;110;51;20735-40

  • A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins.

    Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K et al.

    Molecular & cellular proteomics : MCP 2013;12;12;3976-86

  • Biochemical analysis of the Plasmodium falciparum erythrocyte-binding antigen-175 (EBA175)-glycophorin-A interaction: implications for vaccine design.

    Wanaguru M, Crosnier C, Johnson S, Rayner JC and Wright GJ

    The Journal of biological chemistry 2013;288;45;32106-17

  • A full-length recombinant Plasmodium falciparum PfRH5 protein induces inhibitory antibodies that are effective across common PfRH5 genetic variants.

    Bustamante LY, Bartholdson SJ, Crosnier C, Campos MG, Wanaguru M et al.

    Vaccine 2013;31;2;373-9

  • Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41.

    Taechalertpaisarn T, Crosnier C, Bartholdson SJ, Hodder AN, Thompson J et al.

    PloS one 2012;7;7;e41937

  • Semaphorin-7A is an erythrocyte receptor for P. falciparum merozoite-specific TRAP homolog, MTRAP.

    Bartholdson SJ, Bustamante LY, Crosnier C, Johnson S, Lea S et al.

    PLoS pathogens 2012;8;11;e1003031

  • The impact of gene expression regulation on evolution of extracellular signaling pathways.

    Charoensawan V, Adryan B, Martin S, Söllner C, Thisse B et al.

    Molecular & cellular proteomics : MCP 2010;9;12;2666-77

  • Construction of a large extracellular protein interaction network and its resolution by spatiotemporal expression profiling.

    Martin S, Söllner C, Charoensawan V, Adryan B, Thisse B et al.

    Molecular & cellular proteomics : MCP 2010;9;12;2654-65

  • A rapid and scalable method for selecting recombinant mouse monoclonal antibodies.

    Crosnier C, Staudt N and Wright GJ

    BMC biology 2010;8;76

  • Hypokalemic periodic paralysis associated with thyrotoxicosis, renal tubular acidosis and nephrogenic diabetes insipidus.

    Im EJ, Lee JM, Kim JH, Chang SA, Moon SD et al.

    Endocrine journal 2010;57;4;347-50

  • Signal initiation in biological systems: the properties and detection of transient extracellular protein interactions.

    Wright GJ

    Molecular bioSystems 2009;5;12;1405-12

  • A cell surface interaction network of neural leucine-rich repeat receptors.

    Söllner C and Wright GJ

    Genome biology 2009;10;9;R99

  • Large-scale screening for novel low-affinity extracellular protein interactions.

    Bushell KM, Söllner C, Schuster-Boeckler B, Bateman A and Wright GJ

    Genome research 2008;18;4;622-30

  • Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function.

    Wright GJ, Puklavec MJ, Willis AC, Hoek RM, Sedgwick JD et al.

    Immunity 2000;13;2;233-42