Sanger Institute, Genome Research Limited
We are developing and applying methods to explore the genome, epigenome and transcriptome of single cells in order to better understand normal development and disease processes.
We are part of the wider Illumina sequencing team that generates data for all of the faculty research programmes at Sanger.
In the Bespoke team, we use our resources to support samples that cannot be handled by processes in the high throughput team. This might be due to insufficient DNA, poor sample performance within standard processes or a requirement for a novel library type.
In this way, we spend more resources per sample in order to generate the sequencing data at the required quality.
Our current library types:
WGS, no PCR, Stranded RNA, ChIP (all manual and semi automated) Small RNA, qPCR only (quantification only)
Single cell epigenomics applied to development and ageing
The group seeks to elucidate the principles of protein structure evolution, higher order protein structure and protein folding, and the principles underlying protein complex formation and organization. We have a longstanding interest in understanding gene expression regulation, and in our wetlab at the Sanger Institute use mouse T helper cells as a model of cell differentiation.
Thierry Voet's group focuses on developing methods that characterise the DNA and RNA in a single cell to enable the exploration of DNA-mutation, the genetic differences between cells in a person's body and the relation of this diversity to disease.