New Pipeline Group (NPG)

Sequencing Informatics

NPG is responsible for the delivery of DNA Pipelines's data products and the provision of informatics expertise and QC systems.

Illumina Sequencing forms the majority of DNA Pipelines’ products and NPG provides the tracking, QC, and analysis systems to process the output. Our automated high-throughput analysis systems provide analyses appropriate for the study, sample, and type of library being sequenced. When requested, the team may also provide bespoke or tailored analysis for our faculty customers (for example, 10X processing using other experimental data).

DNA Pipelines typically uses PacBio Sequencing to provide high base quality long-read sequencing in up to 15 kb fragments. NPG wraps around the analysis systems provided by PacBio to ensure data delivery and QC reporting. We also provide local informatics expertise concerning this data.

DNA Pipelines also provide a variety of other sequence assay products: ONT sequencing offers unique capabilities, Bionano a non-sequencing method of studying structure, and small panel genotyping using Fluidigm. NPG wraps around these to ensure automated delivery of suitable data products to the customer.

NPG provides consistent delivery of all the varied data products with appropriate metadata and access control.

The NPG team liaises with DNA Pipelines and faculty informatics to gather requirements and best practices. DNA Pipelines ensure we’re aware of changes in the nature of the product and demand e.g. new instruments, new instrument types, and new projects with expected throughput and processing requirements.

NPG continuously reviews its systems with an eye to product trends and implements long rolling projects to accommodate them.

The NPG team has to maintain informatics, Illumina sequencing, and genotyping knowledge sufficient to anticipate and understand DNA Pipelines customer requirements so that requests can be anticipated, evaluated, and rejected or implemented appropriately.