Mouse Transgenic Technologies | Mouse Pipelines

Mouse Transgenic Technologies | Mouse Pipelines

Mouse Transgenic Technologies

Our Research and Approach

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1 cell mouse embryo ready for injection with a CRISPR/Cas endonuclease

The Transgenic Technologies team is part of mouse pipelines and is repsonsible for the production of genetically modified mice. One of the most important tools at our scientific disposal in understanding mammalian gene function in the laboratory mouse. The fundamental genetic similarity between mice and humans allows researchers to infer a human gene's function based on studies with laboratory mice. One powerful technique is to turn off, or "knockout", the activity of a mouse gene to assess what biological systems are impacted. This gives insights how a similar gene in humans may contribute to disease when its activity is altered. The Transgenic Technologies mouse production team at the Sanger Institute contributes to this global effort by generating knock-out mice for the International Mouse Phenotyping Consortium as well as our own faculty scientists. We at all times consider animal welfare in our procedures and processes and follow the 3Rs' ethos of Replacement, Refinement and Reduction. All work performed using mice are tightly regulated by the Home Office.

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People

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Dr Ramiro Ramirez-Solis, PhD
Group Leader

I work with a large team of superb scientists to generate mutant mouse strains carrying alleles of biomedical importance. Our work includes design of the mutation, mutagenesis of mouse ES cells, CRISPR mutagenesis, transgenic technologies to transfer the allele into the germline, molecular analysis and genotyping, colony management, cryopreservation, phenotypic analysis, and strain distribution.

Key Projects, Collaborations, Tools & Data

Mouse mutant lines are generated primarily for Sanger Institute Mouse Pipelines (formerly Mouse Genetics Project MGP). We have utilised these mutants in key international consortia collaborations such as the International Knockout Mouse Consortia (IKMC), International Mouse Phenotyping Consortia (IMPC), The European Conditional Mouse Mutagenesis Program (EUCOMM). All lines are then deposited and made freely available at The European Mouse Mutant Archive for further investigation by the wider scientifc community.

Partners and Funders

Internal Partners

Publications

  • Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase.

    Ryder E, Doe B, Gleeson D, Houghton R, Dalvi P et al.

    Transgenic research 2014;23;1;177-85

  • Blastocyst genotyping for quality control of mouse mutant archives: an ethical and economical approach.

    Scavizzi F, Ryder E, Newman S, Raspa M, Gleeson D et al.

    Transgenic research 2015;24;5;921-7

  • Juno is the egg Izumo receptor and is essential for mammalian fertilization.

    Bianchi E, Doe B, Goulding D and Wright GJ

    Nature 2014;508;7497;483-7

  • The mammalian gene function resource: the International Knockout Mouse Consortium.

    Bradley A, Anastassiadis K, Ayadi A, Battey JF, Bell C et al.

    Mammalian genome : official journal of the International Mammalian Genome Society 2012;23;9-10;580-6

  • A gene expression resource generated by genome-wide lacZ profiling in the mouse.

    Tuck E, Estabel J, Oellrich A, Maguire AK, Adissu HA et al.

    Disease models & mechanisms 2015;8;11;1467-78

  • Analysis of mammalian gene function through broad-based phenotypic screens across a consortium of mouse clinics.

    de Angelis MH, Nicholson G, Selloum M, White J, Morgan H et al.

    Nature genetics 2015;47;9;969-978

  • Mouse large-scale phenotyping initiatives: overview of the European Mouse Disease Clinic (EUMODIC) and of the Wellcome Trust Sanger Institute Mouse Genetics Project.

    Ayadi A, Birling MC, Bottomley J, Bussell J, Fuchs H et al.

    Mammalian genome : official journal of the International Mammalian Genome Society 2012;23;9-10;600-10

  • Histopathology reveals correlative and unique phenotypes in a high-throughput mouse phenotyping screen.

    Adissu HA, Estabel J, Sunter D, Tuck E, Hooks Y et al.

    Disease models & mechanisms 2014;7;5;515-24

  • Molecular characterization of mutant mouse strains generated from the EUCOMM/KOMP-CSD ES cell resource.

    Ryder E, Gleeson D, Sethi D, Vyas S, Miklejewska E et al.

    Mammalian genome : official journal of the International Mammalian Genome Society 2013;24;7-8;286-94

  • Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen.

    Liakath-Ali K, Vancollie VE, Heath E, Smedley DP, Estabel J et al.

    Nature communications 2014;5;3540

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.

    White JK, Gerdin AK, Karp NA, Ryder E, Buljan M et al.

    Cell 2013;154;2;452-64

  • A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains.

    Simon MM, Greenaway S, White JK, Fuchs H, Gailus-Durner V et al.

    Genome biology 2013;14;7;R82

  • Experimental and husbandry procedures as potential modifiers of the results of phenotyping tests.

    Gerdin AK, Igosheva N, Roberson LA, Ismail O, Karp N et al.

    Physiology & behavior 2012;106;5;602-11

  • Rapid-throughput skeletal phenotyping of 100 knockout mice identifies 9 new genes that determine bone strength.

    Bassett JH, Gogakos A, White JK, Evans H, Jacques RM et al.

    PLoS genetics 2012;8;8;e1002858

  • A conditional knockout resource for the genome-wide study of mouse gene function.

    Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W et al.

    Nature 2011;474;7351;337-42