The Mouse Pipelines team supports biomedical research through the humane and optimized use of genetically-modified mice. Under the most stringent care for the welfare of the animals, the team generates, phenotypes, cryopreserves, and distributes new mouse strains to galvanize biomedical research. This work supports the research efforts of the Sanger Institute Faculty, large international projects like EUCOMM, EUCOMMTools, Infrafrontier, Knock-Out Mouse Project, and Wellcome Trust Strategic Awards. The main technologies employed for the generation of the new strains are gene targeting in mouse embryonic stem cell (mESC) and CRISPR-mediated mutagenesis by zygote cytoplasmic microinjection. All the strains are available to the scientific community worldwide as soon as they have passed appropriate quality control. Strains are preserved as frozen sperm and deposited at the main repositories in Europe and the US for long term distribution. The effect of each mutation is also analyzed through a standard set of phenotyping tests and conditions. The phenotypic data is distributed to the scientific community through the portal of the International Mouse Phenotyping Consortium (IMPC).