Mouse Phenotyping | Mouse Pipelines

Mouse Phenotyping | Mouse Pipelines

Mouse Phenotyping

Our Research and Approach

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The Mouse Phenotyping team delivers phenotypic characterisation of mutant mice, typically knockouts of protein coding genes. Phenotyping encompasses a standardised set of more than 600 clinical parameters, and covers key biomedical areas such as reproduction, development, infection and immunity, musculoskeletal system, metabolism and endocrinology.

Characterisation is complete for over 1000 mutant mouse lines, with an additional 160 strains processed annually. All the data are freely available to the scientific community.

We find many unexpected phenotypes detected only because we screened for them, emphasizing the value of screening all mutants for a wide range of traits. This pipeline of knockout mouse generation and phenotyping is world-leading in its effectiveness, sophistication and throughput.

Our mouse strains and data are used by scientists throughout the world, reducing the amount of unnecessary experimental duplication and ensuring a 'gold standard' of models for research groups to study.

People

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Dr Ramiro Ramirez-Solis, PhD
Group Leader

I work with a large team of superb scientists to generate mutant mouse strains carrying alleles of biomedical importance. Our work includes design of the mutation, mutagenesis of mouse ES cells, CRISPR mutagenesis, transgenic technologies to transfer the allele into the germline, molecular analysis and genotyping, colony management, cryopreservation, phenotypic analysis, and strain distribution.

Partners and Funders

Internal Partners
External Partners and Funders

Publications

  • A gene expression resource generated by genome-wide lacZ profiling in the mouse.

    Tuck E, Estabel J, Oellrich A, Maguire AK, Adissu HA et al.

    Disease models & mechanisms 2015;8;11;1467-78

  • Analysis of mammalian gene function through broad-based phenotypic screens across a consortium of mouse clinics.

    de Angelis MH, Nicholson G, Selloum M, White J, Morgan H et al.

    Nature genetics 2015;47;9;969-978

  • Applying the ARRIVE Guidelines to an In Vivo Database.

    Karp NA, Meehan TF, Morgan H, Mason JC, Blake A et al.

    PLoS biology 2015;13;5;e1002151

  • Histopathology reveals correlative and unique phenotypes in a high-throughput mouse phenotyping screen.

    Adissu HA, Estabel J, Sunter D, Tuck E, Hooks Y et al.

    Disease models & mechanisms 2014;7;5;515-24

  • Impact of temporal variation on design and analysis of mouse knockout phenotyping studies.

    Karp NA, Speak AO, White JK, Adams DJ, Hrabé de Angelis M et al.

    PloS one 2014;9;10;e111239

  • Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen.

    Liakath-Ali K, Vancollie VE, Heath E, Smedley DP, Estabel J et al.

    Nature communications 2014;5;3540

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.

    White JK, Gerdin AK, Karp NA, Ryder E, Buljan M et al.

    Cell 2013;154;2;452-64

  • A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains.

    Simon MM, Greenaway S, White JK, Fuchs H, Gailus-Durner V et al.

    Genome biology 2013;14;7;R82

  • Experimental and husbandry procedures as potential modifiers of the results of phenotyping tests.

    Gerdin AK, Igosheva N, Roberson LA, Ismail O, Karp N et al.

    Physiology & behavior 2012;106;5;602-11

  • Rapid-throughput skeletal phenotyping of 100 knockout mice identifies 9 new genes that determine bone strength.

    Bassett JH, Gogakos A, White JK, Evans H, Jacques RM et al.

    PLoS genetics 2012;8;8;e1002858