The Mouse Phenotyping team delivers phenotypic characterisation of mutant mice, typically knockouts of protein coding genes. Phenotyping encompasses a standardised set of more than 600 clinical parameters, and covers key biomedical areas such as reproduction, development, infection and immunity, musculoskeletal system, metabolism and endocrinology.
Characterisation is complete for over 1000 mutant mouse lines, with an additional 160 strains processed annually. All the data are freely available to the scientific community.
We find many unexpected phenotypes detected only because we screened for them, emphasizing the value of screening all mutants for a wide range of traits. This pipeline of knockout mouse generation and phenotyping is world-leading in its effectiveness, sophistication and throughput.
Our mouse strains and data are used by scientists throughout the world, reducing the amount of unnecessary experimental duplication and ensuring a 'gold standard' of models for research groups to study.
I work with a large team of superb scientists to generate mutant mouse strains carrying alleles of biomedical importance. Our work includes design of the mutation, mutagenesis of mouse ES cells, CRISPR mutagenesis, transgenic technologies to transfer the allele into the germline, molecular analysis and genotyping, colony management, cryopreservation, phenotypic analysis, and strain distribution.
We are a contributing partner in the Consortium, which aims to discover the function of all of the genes in the mouse genome that code for proteins by the use of a broad-spectrum standardised phenotyping platform and data set