Model Pipelines are headed by Ramiro Ramirez-Solis and produce and characterise knock-out mice for large-scale research projects, including the Sanger Mouse Genetics Project, National Institutes of Health KOMP2, EUCOMMTools, and the Infrafrontier projects.
In addition the team is closely involved in three major Strategic Award projects funded by the Wellcome Trust: • Deciphering the Mechanisms of Developmental Disorders (DMDD) • Origins of Bone and Cartilage Disease (OBCD) • Immune function and pathology dissected by high-throughput analysis of mice with targeted gene disruptions – an investigation by the Infection and Immunity Immunophenotyping (3i) consortium.
We serve the scientific community with genetically altered strains of mice by supplying public repositories and researchers directly. Availability of these mouse strains is complemented by the standardised phenotypic characterisation performed by Mouse Pipelines, that is freely available to the scientific community. Accordingly, the research community continues to produce a growing scientific output using the resources distributed from the Mouse Pipelines at the Institute. To support this important part of our mission, we have an office to carry out cost-recovery.
The team researches and develops mouse resource production methods. For example we are implementing the use of the CRISPR mouse mutagenesis technology directly in mouse embryos, which will lead to great savings and acceleration of projects in their early phase by removing the need to use embryonic stem cells. We work flexibly so that we can rapidly shift our resources to advance all large-scale projects as needed while also supporting the needs of individual Faculty members.
We work closely with the Mouse Informatics and Research Support Facility teams to develop the Mouse Database. This work is essential for the welfare of our mice, to maintain optimal operational workflows, and to achieve our scientific goals, as well as for streamlining publication of our scientific results through the International Mouse Phenotyping Consortium (IMPC) web portal.
I work with a large team of superb scientists to generate mutant mouse strains carrying alleles of biomedical importance. Our work includes design of the mutation, mutagenesis of mouse ES cells, CRISPR mutagenesis, transgenic technologies to transfer the allele into the germline, molecular analysis and genotyping, colony management, cryopreservation, phenotypic analysis, and strain distribution.